점망둑 (Chasmichthys dolichognathus)의 성숙기 난모세포에서의 C₂₁-스테로이드 생성
Production of C₂₁-steroids in longchin goby, Chasmichthys dolichognathus during oocyte maturation
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초록
점망둑의 난모세포 성숙과정에서 생성되는 주요 성 스테로이드 호르몬, C21-스테로이드를 분석하고자 전구물질 3H-17αhydroxyprogesterone (3H-17αOHP)를 성숙기 난모세포(난경 0.74-0.97 mm) 배양초기에 첨가하여 24시간 배양하였다. 스테로이드 대사물질 분석과 동정은 thin layer chromatography와 gaschromatography-mass spectrometry로 이루어졌다. 3H-17αOHP로부터 생성된 주요 성 스테로이드 대사물질은 17α-hydroxy,20α-dihydro progesterone (17α20αP)와 17α-hydroxy,20β-dihydroprogesterone (17α20βP)로 확인되었다. 이들 대사물질은 난경 0.80 mm 이상에서 관찰되었으며, GVBD (germinal vesicle breakdown) 유도 효능 테스트에서 점망둑의 난모세포는 17α20βP에 더 민감하게 반응하였다. 이러한 결과는 점망둑의 난소성숙 과정에 17α20αP와 17α20βP이 모두 관여하나 MIS (maturation inducing steroid)로서의 가능성은 17α20βP이 더 큰 것으로 관찰되었다.
Abstract
To investigate the C21-steroids produced from maturating oocytes in the longchin goby, Chasmichthys dolichognathus, the oocytes (0.74-0.97 mm) were incubated with radiolabeled 17α-hydroxyprogesterone (3H-17αOHP) for 24 hours. The resulting metabolites were analyzed by thin layer chromatography and identified by gas chromatography-mass spectrometry. Two C21-steroids, 17α-hydroxy,20α-dihydroprogesterone (17α20αP) and 17α-hydroxy,20β-dihydroprogesterone (17α20βP), were converted from 3H-17αOHP in the maturing oocytes. These two main metabolites were detected at 0.80 mm diameter oocytes or greater. In addition, the effects of these metabolites on in vitro germinal vesicle breakdown (GVBD) were tested. The sensitivity of oocytes to the induction of GVBD was greater at 17α20βP than 17α20αP. This result showed that 17α20βP is a major maturation inducing steroid (MIS) in longchin goby, suggesting 17α20αP may play a role in regulating the oocyte maturation process.
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