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Optimization and production of protein hydrolysate containing antioxidant activity from tuna cooking juice concentrate by response surface methodology

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초록

To optimize the hydrolysis conditions in the production of antioxidant hydrolysates from tuna cooking juice concentrate (TC) to maximize the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, TC containing 48.91% protein was hydrolyzed with Alcalase 2.4 L, and response surface methodology (RSM) was applied. The optimum hydrolysis conditions included a 2.2% (w/v) Alcalase concentration and 281 min hydrolysis time, resulting in the highest DPPH radical scavenging activity of 66.49% (0.98 μmol Trolox/mg protein). The analysis of variance for RSM showed that hydrolysis time was an important factor that significantly affected the process (p < 0.05). The effects of different drying methods (freeze drying, hot air drying, and vacuum drying) on the DPPH radical scavenging activity and amino acid (AA) profiles of TC hydrolysate (TCH) were evaluated. Vacuum-dried TCH (VD) exhibited an increase in DPPH radical scavenging activity of 81.28% (1.20 μmol Trolox/mg protein). The VD samples were further fractionated by ultrafiltration. The AA profiles and antioxidant activities in terms of the DPPH radical scavenging activity, 2,2’-azino- bis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radical scavenging activity, ferric reducing antioxidant power, and ferrous ion chelating activity were investigated. Glutamic acid, glycine, arginine, and cysteine were the major AAs found in the TCH fractions. The highest DPPH radical scavenging activity was found in the VD-1 fraction (< 5 kDa). The VD-3 fraction (> 10 kDa) exhibited the highest ABTS radical scavenging activity and ferric reducing antioxidant power. The ferrous ion chelating activity was the highest in VD-1 and VD-2 (5 to 10 kDa). In conclusion, this study provided the optimal conditions to obtain high antioxidant activities through TCH production, and these conditions could provide a basis for the future application of TCH as a functional food ingredient.

Abstract

To optimize the hydrolysis conditions in the production of antioxidant hydrolysates from tuna cooking juice concentrate (TC) to maximize the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, TC containing 48.91% protein was hydrolyzed with Alcalase 2.4 L, and response surface methodology (RSM) was applied. The optimum hydrolysis conditions included a 2.2% (w/v) Alcalase concentration and 281 min hydrolysis time, resulting in the highest DPPH radical scavenging activity of 66.49% (0.98 μmol Trolox/mg protein). The analysis of variance for RSM showed that hydrolysis time was an important factor that significantly affected the process (p < 0.05). The effects of different drying methods (freeze drying, hot air drying, and vacuum drying) on the DPPH radical scavenging activity and amino acid (AA) profiles of TC hydrolysate (TCH) were evaluated. Vacuum-dried TCH (VD) exhibited an increase in DPPH radical scavenging activity of 81.28% (1.20 μmol Trolox/mg protein). The VD samples were further fractionated by ultrafiltration. The AA profiles and antioxidant activities in terms of the DPPH radical scavenging activity, 2,2’-azino- bis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radical scavenging activity, ferric reducing antioxidant power, and ferrous ion chelating activity were investigated. Glutamic acid, glycine, arginine, and cysteine were the major AAs found in the TCH fractions. The highest DPPH radical scavenging activity was found in the VD-1 fraction (< 5 kDa). The VD-3 fraction (> 10 kDa) exhibited the highest ABTS radical scavenging activity and ferric reducing antioxidant power. The ferrous ion chelating activity was the highest in VD-1 and VD-2 (5 to 10 kDa). In conclusion, this study provided the optimal conditions to obtain high antioxidant activities through TCH production, and these conditions could provide a basis for the future application of TCH as a functional food ingredient.

참고문헌 (55)

  1. Alahmad K, "Effect of the degree of hydrolysis on nutritional, functional, and morphological characteristics of protein hydrolysate produced from bighead carp(Hypophthalmichthys nobilis)using ficin enzyme", Foods, 11, pp.1320~ (2022)
  2. Alemán A, "Squid gelatin hydrolysates with antihypertensive, anticancer and antioxidant activity", Food Res Int, 44, pp.1044~1051 (2011)
  3. Association of Official Analytical Chemists, "Official methods of analysis of AOAC International" (2000), AOAC International
  4. Auwal SM, "Response surface optimisation for the production of antioxidant hydrolysates from stone fish protein using bromelain", Evid Based Complement Alternat Med, 2017, pp.4765463~ (2017)
  5. Azemi WAWM, "Bioactivity of enzymatically prepared eel(Monopterus sp. )protein hydrolysate at different molecular weights", Int Food Res J, 24, pp.571~578 (2017)
  6. Bahari AN, "Response factorial design analysis on papain-generated hydrolysates from Actinopyga lecanora for determination of antioxidant and antityrosinase activities", Molecules, 25, pp.2663~ (2020)
  7. Banjongsinsiri P, "Production of mushroom protein hydrolysates by enzymatic hydrolysis and their physicochemical properties", Food Appl Biosci J, 4, pp.161~170 (2017)
  8. Borawska J, "Ex vivo digestion of carp muscle tissue – ACE inhibitory and antioxidant activities of the obtained hydrolysates", Food Funct, 6, pp.211~218 (2015)
  9. Chaijan M, "Characterization of antioxidant peptides from Thai traditional semi-dried fermented catfish", Fermentation, 7, pp.262~ (2021)
  10. Cheng ML, "Anti-inflammatory peptides from enzymatic hydrolysates of tuna cooking juice", Food Agric Immunol, 26, pp.770~781 (2015)
  11. Choudhary AK, "Optimization and validation of process parameters via RSM for minimizing use of resources to generate electricity from a DEFC", Int J Energy Res, 45, pp.20413~20429 (2021)
  12. Chuesiang P, "Protein hydrolysate from tilapia frame : antioxidant and angiotensin I converting enzyme inhibitor properties", Int J Food Sci Technol, 50, pp.1436~1444 (2015)
  13. Ciou JY, "Enhancement of agricultural processed by-products : qualities analysis of fermentation method in gradient salt adding treatment of tuna cooking juice with black bean koji added", Foods, 9, pp.320~ (2020)
  14. Dabbour M, "Effect of drying techniques on the physical, functional, and rheological attributes of isolated sunflower protein and its hydrolysate", Processes, 10, pp.13~ (2022)
  15. Decker EA, "Role of ferritin as a lipid oxidation catalyst in muscle food", J Agric Food Chem, 38, pp.674~677 (1990)
  16. Dong S, "Antioxidant and biochemical properties of protein hydrolysates prepared from silver carp(Hypophthalmichthys molitrix)", Food Chem, 107, pp.1485~1493 (2008)
  17. Food and Agriculture Organization of the United Nations, "Protein and amino acid requirements in human nutrition" (2007), World Health Organization
  18. Halim NRA, "A response surface approach on hydrolysis condition of eel (Monopterus sp.) protein hydrolysate with antioxidant activity", Int Food Res J, 24, pp.1081~1093 (2017)
  19. He R, "Antioxidant activities of enzymatic rapeseed protein hydrolysates and the membrane ultrafiltration fractions", J Funct Foods, 5, pp.219~227 (2013)
  20. Henriques A, "Characterization of protein hydrolysates from fish discards and by-products from the north-west Spain fishing fleet as potential sources of bioactive peptides", Mar Drugs, 19, pp.338~ (2021)
  21. How YK, "Effects of convection-, vacuum-and freeze-drying on antioxidant, physicochemical properties, functional properties and storage stability of stink bean(Parkia speciosa)powder", J Food Sci Technol, 57, pp.4637~4648 (2020)
  22. Jakkranuhwat N, "Effect of foam-mat drying conditions on antioxidant activity, total phenolic compound, anthocyanin content and color of purple-fleshed sweet potato powder", Chiang Mai Univ J Nat Sci, 20, pp.e2021045~ (2021)
  23. Karimi A, "Fractionation of hydrolysate from corn germ protein by ultrafiltration : in vitro antidiabetic and antioxidant activity", Food Sci Nutr, 8, pp.2395~2405 (2020)
  24. Kasiwut J, "Angiotensin I-converting enzyme inhibitory and Ca-binding activities of peptides prepared from tuna cooking juice and spleen proteases", Int J Food Sci Technol, 50, pp.389~395 (2015)
  25. Khositanon P, "Effects of fermentation periods on antioxidant and angiotensin I-converting enzyme inhibitory activities of peptides from fish sauce by-products", LWTFood Sci Technol, 135, pp.110122~ (2021)
  26. Kim JH, "In vitro antioxidant actions of sulfur-containing amino acids", Arab J Chem, 13, pp.1678~1684 (2020)
  27. Kumari M, "Response surface methodological(RSM)approach for optimizing the removal of trihalomethanes(THMs)and its precursor’s by surfactant modified magnetic nanoadsorbents(sMNP)-an endeavor to diminish probable cancer risk", Sci Rep, 9, pp.18339~ (2019)
  28. Automated amino acid analysis using an Agilent Poroshell HPH-C18 column, "Long W", Agilent Technologies, ~, pp.5991-5571EN (2021)
  29. Mahdavi-Yekta M, "The effects of hydrolysis condition on antioxidant activity of protein hydrolyzate from quinoa", Food Sci Nutr, 7, pp.930~936 (2019)
  30. Martínez-Montaño E, "Biochemical and antioxidant properties of recovered solids with pH shift from fishery effluents(sardine stickwater and tuna cooking water)", Waste Biomass Valori, 12, pp.1901~1913 (2021)
  31. Mongkonkamthorn N, "Enzymatic hydrolysis optimization for preparation of tuna dark meat hydrolysate with antioxidant and angiotensin I-converting enzyme(ACE)inhibitory activities", J Aquat Food Prod Technol, 30, pp.1090~1108 (2021)
  32. Narkprasom N, "Optimization of total phenolic from Cleistocalyx nervosum by microwave-assisted extraction", Am J Eng Appl Sci, 8, pp.302~309 (2015)
  33. Novozymes, "Proteases for biocatalysis : for smarter chemical synthesis" (2016), Novozymes
  34. Oyaizu M, "Studies on products of browning reaction antioxidative activities of products of browning reaction prepared from glucosamine", Jpn J Nutr Diet, 44, pp.307~315 (1986)
  35. Parvathy U, "Characterization of fish protein hydrolysate from red meat of Euthynnus affinis and its application as an antioxidant in iced sardine", J Sci Ind Res, 77, pp.111~119 (2018)
  36. Peng S, "Amino acid and fatty acid composition of the muscle tissue of yellowfin tuna(Thunnus albacares)and bigeye tuna(Thunnus obesus)", J Food Nutr Res, 1, pp.42~45 (2013)
  37. Phongthai S, "Fractionation and antioxidant properties of rice bran protein hydrolysates stimulated by in vitro gastrointestinal digestion", Food Chem, 240, pp.156~164 (2018)
  38. Prasertsan P, "Seafood processing industries within Songkhla-Hat Yai region; the survey of basic data emphasis on wastes", Songklanakarin J Sci Technol, 10, pp.447~451 (1988)
  39. Šumić Z, "Modeling and optimization of red currants vacuum drying process by response surface methodology(RSM)", Food Chem, 203, pp.465~475 (2016)
  40. Tacias-Pascacio VG, "Use of Alcalase in the production of bioactive peptides : a review", Int J Biol Macromol, 165, pp.2143~2196 (2020)
  41. Tang X, "Peptide fractionation and free radical scavenging activity of zein hydrolysate", J Agric Food Chem, 58, pp.587~593 (2010)
  42. Tang WL, "Effects of preparation and drying methods on the antioxidant activity of enzymatically hydrolyzed porcine placenta hydrolysates", Dry Technol, 31, pp.1600~1610 (2013)
  43. Tapal A, "Enzymes in food biotechnology: production, applications, and future prospects", pp.471~481 (2019), Academic Press
  44. Udenigwe CC, "Chemometric analysis of the amino acid requirements of antioxidant food protein hydrolysates", Int J Mol Sci, 12, pp.3148~3161 (2011)
  45. Uribe E, "Effect of drying methods on bioactive compounds, nutritional, antioxidant, and antidiabetic potential of brown alga Durvillaea antarctica", Dry Technol, 38, pp.1915~1928 (2020)
  46. Wahid Z, "Improvement of one factor at a time through design of experiments", World Appl Sci J, 21, pp.56~61 (2013)
  47. Wang X, "Optimization of the extraction and stability of antioxidative peptides from Mackerel(Pneumatophorus japonicus)protein", Biomed Res Int, 2017, pp.6837285~ (2017)
  48. Wouters AGB, "Relevance of the functional properties of enzymatic plant protein hydrolysates in food systems", Compr Rev Food Sci Food Saf, 15, pp.786~800 (2016)
  49. Yao H, "Preparation, amino acid composition, and in vitro antioxidant activity of okra seed meal protein hydrolysates", Food Sci Nutr, 9, pp.3059~3070 (2021)
  50. Yen GC, "Antioxidative activity and scavenging effects on active oxygen of xylose-lysine maillard reaction products", J Sci Food Agric, 67, pp.415~420 (1995)
  51. Zamorano-Apodaca JC, "Biological and functional properties of peptide fractions obtained from collagen hydrolysate derived from mixed by-products of different fish species", Food Chem, 331, pp.127350~ (2020)
  52. Zhao L, "Antioxidant activity of protein hydrolysates from aqueous extract of velvet antler(Cervus elaphus)as influenced by molecular weight and enzymes", Nat Prod Commun, 6, pp.1683~1688 (2011)
  53. Zheng L, "Practical problems when using ABTS assay to assess the radical-scavenging activity of peptides : importance of controlling reaction pH and time", Food Chem, 192, pp.288~294 (2016)
  54. Zhuang Y, "Optimization of antioxidant activity by response surface methodology in hydrolysates of jellyfish(Rhopilema esculentum)umbrella collagen", J Zhejiang Univ Sci B, 10, pp.572~579 (2009)
  55. Zou TB, "The structure-activity relationship of the antioxidant peptides from natural proteins", Molecules, 21, pp.72~ (2016)

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