FDY003의 항산화활성 및 표준화 연구
Study on Antioxidant Activity and Standardization of FDY003
0
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Objectives: FDY003 is a raw material for medicine consisting of a natural product that is expected to have the advantages of low side effects and high efficacy. In this study, we predict the efficacy and the standardization of the drug by method validation of anticipated index compounds and the measurement of antioxidant activity. Methods: FDY003 is prepared by extracting and purifying 70% of ethyl alcohol (EtOH). The method validation of cordycepin and chlorogenic acid was determined by high-performance liquid chromatography-photo diode array (HPLC-PDA) and the content of FDY003 was calculated. In order to monitor the biological activity of FDY003, antioxidant activity was measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azino bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and ferric-reducing antioxidant power (FRAP). The equivalent values of antioxidants such as trolox, ascorbic acid, gallic acid, and caffeic acid were measured by ABTS and FRAP. Results: Chlorogenic acid and cordycepin were both found suitable for method validation in HPLC and FDY003 containing 9.92±0.50 and 17.97±0.27 μg/g, respectively. In DPPH, the electron donating ability (EDA) value of FDY003 was increased in a concentration dependent manner. FDY003 confirmed antioxidant activity by ABTS and FRAP. Conclusions: FDY003 contains certain components including cordycepin and chlorogenic acid and has antioxidant ability by various mechanisms. Therefore, it is expected that FDY003 is capable of various physiological activities including anti-cancer activity.
Abstract
Objectives: FDY003 is a raw material for medicine consisting of a natural product that is expected to have the advantages of low side effects and high efficacy. In this study, we predict the efficacy and the standardization of the drug by method validation of anticipated index compounds and the measurement of antioxidant activity. Methods: FDY003 is prepared by extracting and purifying 70% of ethyl alcohol (EtOH). The method validation of cordycepin and chlorogenic acid was determined by high-performance liquid chromatography-photo diode array (HPLC-PDA) and the content of FDY003 was calculated. In order to monitor the biological activity of FDY003, antioxidant activity was measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azino bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and ferric-reducing antioxidant power (FRAP). The equivalent values of antioxidants such as trolox, ascorbic acid, gallic acid, and caffeic acid were measured by ABTS and FRAP. Results: Chlorogenic acid and cordycepin were both found suitable for method validation in HPLC and FDY003 containing 9.92±0.50 and 17.97±0.27 μg/g, respectively. In DPPH, the electron donating ability (EDA) value of FDY003 was increased in a concentration dependent manner. FDY003 confirmed antioxidant activity by ABTS and FRAP. Conclusions: FDY003 contains certain components including cordycepin and chlorogenic acid and has antioxidant ability by various mechanisms. Therefore, it is expected that FDY003 is capable of various physiological activities including anti-cancer activity.
참고문헌 (33)
- 원혜련, "창출금련탕(蒼朮芩連湯) 추출물의 항산화 및 항염증 활성에 미치는 영향", 한방안이비인후피부과학회지, 31(4), pp.1~12 (2018), 대한한방안이비인후피부과학회[DOI]
- 김유진, "차전초 에탄올 추출물의 항산화 효능", 대한본초학회지, 33(3), pp.37~43 (2018), 대한본초학회[DOI]
- 이강욱, "韓茵蔯, 鬱金, 山査, 丹蔘 발효복합추출물이 RAW264.7 및 HUVEC 세포에서 나타나는 이상지질혈증 관련 염증인자 발현 및 항산화에 미치는 영향", 대한한방내과학회지, 39(4), pp.480~494 (2018), 대한한방내과학회
- Kehrer JP, "Free radicals and related reactive species as mediators of tissue injury and disease:implications for Health", Crit Rev Toxicol, 40(9), pp.765~798 (2015)
- 신정희, "금은화 추출물을 이용한 미생물 발효 생성물의 항산화 특성", 동아시아식생활학회지, 22(1), pp.95~102 (2012), 동아시아식생활학회
- Hong M, "A network-based pharmacology study of the herb-induced liver injury potential of traditional hepatoprotective Chinese herbal medicines", Molecule, 22(4), pp.632~ (2017)
- Lee IH, "FDY003 inhibits colon cancer in a colo205 xenograft mouse model by decreasing oxidative stress", Pharmacogn Mag, 65(15), pp.675~681 (2019)
- Chaowuttikul C, "Pharmacognostic specification, chlorogenic acid content, and in vitro antioxidant activities of Lonicera aponica Flowering Bud", Pharmacognosy Res, 9(2), pp.128~132 (2017)
- 서성숙, "발효 금은화의 인플루엔자 바이러스 A형에 대한 저해효과", 대한한방내과학회지, 30(3), pp.465~480 (2009), 대한한방내과학회
- Park C, "Lonicera japonica Thunb. Induces caspase-dependent apoptosis through death receptors and suppression of AKT in U937 human leukemic cells", Phytother Res, 32(3), pp.504~513 (2018)
- Han JM, "Effects of Lonicera japonica Thunb. on type 2 diabetes via PPAR-γactivation in rats", Phytother Res, 29(10), pp.1616~1621 (2015)
- 유은주, "Artemisia capillaries Herbal Acupuncture Improves Metabolic Abnormalities in High Fat Diet-induced Obese ICR Mice", Journal of Acupuncture Research, 28(1), pp.1~14 (2011), 대한침구의학회
- 김정숙, "인진호 추출물의 항산화활성 및 항염효과", 아시안뷰티화장품학술지, 13(6), pp.805~812 (2015), 한국피부과학연구원
- Jang E, "Evaluation of antitumor activity of Artemisia capillaris extract against hepatocellular carcinoma through the inhibition of IL-6", STAT3 signaling axis, 2017, pp.37(1), 526~532
- Son HU, "The solid-state fermentation of Artemisia capillaris leaves with anoderma lucidum enhances the anti-inflammatory effects in a model of atopic dermatitis", Int J Mol Med, 39(5), pp.1233~1241 (2017)
- 이현희, "Characterization of Newly Bred Cordyceps militaris Strains for Higher Production of Cordycepin through HPLC and URP-PCR Analysis", Journal of Microbiology and Biotechnology, 27(7), pp.1223~1232 (2017), 한국미생물·생명공학회[DOI]
- Im WH, "Study of fermentation condition of anti-oxidant and anti-inflammatory effects of starfish extract fermented by Cordyceps militaris", Korea Immuno-Yakchim Society, 4(2), pp.27~36 (2015)
- Wang M, "Cordyceps militaris polysaccharides can enhance the immunity and antioxidation activity in immunosuppressed mice", Carbohydr Polym, 89(2), pp.461~466 (2012)
- 김연숙, "Anti-inflammatory effect of the extract from fermented Asterina pectinifera with Cordyceps militaris mycelia in LPS-induced RAW264.7 macrophages", Food Science and Biotechnology, 26(6), pp.1633~1640 (2017), 한국식품과학회[DOI]
- Jin Y, "Anti-tumor and anti-metastatic roles of cordycepin, one bioactive compound of Cordyceps militaris", Saudi J Biol Sci, 25(5), pp.991~995 (2018)
- Zhou X, "Separation of cordycepin from Cordyceps militaris fermentation supernatant using preparative HPLC and evaluation of its antibacterial activity as an NAD+-dependent DNA ligase inhibitor", Exp Ther Med, 12(3), pp.1812~1816 (2016)
- 송선영, "금은화의 폴리페놀, 플라보노이드, Chlorogenic acid의 최적 추출조건", 공학기술논문지, 11(1), pp.1~5 (2018), 공학기술연구원[DOI]
- Man SC, "Systematic review on the efficacy and safety of herbal medicines for alzheimer's disease", J Alzheimers Dis, 14(2), pp.209~223 (2008)
- i Mobilization via cAMP-Dependent Phosphorylation of Inositol 1, 4, 5-Trisphosphate Receptor in Human Platelets, "Biomolecules & Therapeutics", 2014, 223~231, pp.한국응용약물학회
- Bauer G, "The antitumor effect of singlet oxygen", Anticancer Res, 36(11), pp.5649~5663 (2016)
- 권구중, "화살나무 잎 열수추출물의 생리활성", 한국식품과학회지, 39(5), pp.569~574 (2007), 한국식품과학회
- 최준혁, "벌나무 열수 및 에탄올 추출물의 항산화 활성 및 알코올 분해능", 한국식품영양과학회지, 42(3), pp.378~383 (2013), 한국식품영양과학회
- 강경명, "추출 방법이 개두릅 추출물의 항산화 활성에 미치는 영향", 한국식품영양과학회지, 41(12), pp.1686~1692 (2012), 한국식품영양과학회
- 정세현, "약재 추출물을 첨가한 과립의 품질 특성 및 항산화 효과", 한국식품영양과학회지, 44(9), pp.1311~1316 (2015), 한국식품영양과학회
- 김혜숙, "참외 비식용부위(꼭지, 줄기․잎, 씨) 에탄올추출물의 항산화 활성", 한국자원식물학회지, 23(5), pp.451~457 (2010), 한국자원식물학회
- 주신윤, "약용식물 추출물의 항산화 효과", 한국식품영양과학회지, 42(4), pp.512~519 (2013), 한국식품영양과학회
- 하현주, "적양배추 추출물의 항산화 및 항염증 활성", Culinary Science & Hospitality Research, 20(2), pp.16~26 (2014), (사)한국조리학회[DOI]
- 이민영, "과일 껍질의 비타민 C , 폴리페놀, 플라보노이드 함량과 항산화 활성", 한국식품과학회지, 44(5), pp.540~544 (2012), 한국식품과학회[DOI]