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Cloning and Characterization of Two Distinct CD3 Genes from Olive Flounder Paralichthys olivaceus

박찬일|김무찬
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)|2005.06|Fisheries and Aquatic Sciences|제8권 제2호|pp. 56-64

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참고문헌

주제분류수산학

초록

Abstract

Two distinct CD3 homologue genes, CD3 and CD, were isolated from a olive flounder leukocyte cDNA library and a BAC library. CD3 consisted of 961 bp encoding 178 amino acid residues, and CD3 consisted of 1006 bp encoding 164 amino acid residues. When compared with other known CD3 peptide sequences, the most conserved region of the two olive flounder CD3 chain peptides are the cytoplasmic domain and the least conserved are the extracellular domain. A phylogenetic analysis based on the deduced amino acid sequence grouped the two olive flounder CD3 sequences with CD3 and CD3, respectively. The olive flounder CD3 cluster (consisting of CD3 and CD3) spans only 10.4 kb. The CD3 and CD3 genes are oppositely transcribed only 3.8 kb apart. Both olive flounder CD3 genes have five exons. The two olive flounder CD3 genes were predominantly expressed in PBLs, kidney, spleen, and gills.

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