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Generation of polyclonal antiserum to olive flounder (Paralichthys olivaceus) immunoglobulin by immunization of rabbit with plasmids containing heavy chain gene of olive flounder immunoglobulin

이은혜|권세련|김기홍|Chun Soo Kjm
수산생명의학회 (Aquatic Life Medical Science)|2006.08|한국어병학회지 (JOURNAL OF FISH PATHOLOGY)|제19권 제2호|pp. 183-188

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Abstract

In fish vaccinology, the secondary antibodies against fish immunoglobulins (Igs) are necessary to measure specific humoral immune responses in immunized fish. In the present study, polyclonal antiserum against olive flounder (Paralichthys olivaceus) IgM heavy chain was generated by intramuscular immunization of rabbit with Escherichia coli/eukaryotic shuttle vector containing open reading frame (ORF) of olive flounder IgM heavy chain. Western blot analysis demonstrated the specific activity of the rabbit antiserum with reduced olive flounder serum H chain at dilutions up to 1:1000. Titer of immunized rabbit serum against olive flounder serum was significantly higher than that of pre-immunized rabbit serum when determined by ELISA.

참고문헌 (24)

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관련 논문AI추천

넙치(Paralichthys olivaceus)의 immunoglobulin M에 대한 단클론 항체 생산김기홍, 김위식, 김춘섭, 오명주한국수산과학회 · 한국수산과학회지2017 · 제50권 제2호Survey of the Expression Pattern and Immuno Stimulatory Effect of DNA Vaccine Using β-Galactosidase Reporter System in Olive Flounder (Paralichthys olivaceus)김영옥, 안경진, 이상준, 홍수희한국수산과학회 · Fisheries and Aquatic Sciences2004 · 제7권 제2호Cloning and Characterization of Two Distinct CD3 Genes from Olive Flounder Paralichthys olivaceus김무찬, 박찬일한국수산과학회 · Fisheries and Aquatic Sciences2005 · 제8권 제2호Effects of CpG Motifs Present in Synthetic Oligodeoxynucleotides on Nonspecific Immune Responses and Disease Resistance of Olive Flounder (Paralichthys olivaceus)이은혜, ChanHweiLee, KiHongKim, SeRyunKwon한국수산과학회 · Fisheries and Aquatic Sciences2003 · 제6권 제3호Production of Phagocyte Activating Supernatants by Olive Flounder (Paralichthys olivaceus) Leucocytes Stimulated with Genomic DNA of Escherichia coliChanHweiLee, DongSooKim, KiHongKim한국수산과학회 · Fisheries and Aquatic Sciences2002 · 제5권 제4호Effects of CpG Oligodexynucleotides on Immune Responses and Expression of Cytokine Genes in Cultured Olive Flounder Paralichthys olivaceus강정하, 김봉석, 김영옥, 남보혜, 안경진, 이상준, 지영주한국수산과학회 · Fisheries and Aquatic Sciences2007 · 제10권 제1호

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