RAG-1 and IgM Genes, Markers for Early Development of the Immune System in Olive Flounder, Paralichthys olivaceus
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초록
Fish larvae are immediately exposed to microbes from hatching to maturation of their lymphoid organs,therefore effective innate mechanisms is very important for survival. However, the knowledge of the development of immunesystem in fish is limited and in demand now. In vertebrates, recombination-activating gene 1 (RAG-1) and immunoglobulinM (IgM) have been considered as very useful markers of the physiological maturity of the immune system. In this study, theexpression of the both genes was assessed throughout the early developmental stages of olive flounder larvae (5-55 dph) andused as markers to follow the development of immune system. RAG-1 and IgM mRNA expression was detectable at 5 dphand remained so until 55 dph. These patterns of expression may suggest that the olive flounder start to develop its functionaround 5 dph. Tissue distribution was found that both genes mRNAs are only expressed in the immune-related organ such asspleen, kidney and gill. The early detection of IgM mRNA led to the investigation of its presence in oocytes. Both RAG-1 andIgM mRNA transcripts were detected in unfertilized oocytes, suggesting that they are maternally transferred. The biologicalsignificance of such a phenomenon remains to be investigated.
Abstract
Fish larvae are immediately exposed to microbes from hatching to maturation of their lymphoid organs,therefore effective innate mechanisms is very important for survival. However, the knowledge of the development of immunesystem in fish is limited and in demand now. In vertebrates, recombination-activating gene 1 (RAG-1) and immunoglobulinM (IgM) have been considered as very useful markers of the physiological maturity of the immune system. In this study, theexpression of the both genes was assessed throughout the early developmental stages of olive flounder larvae (5-55 dph) andused as markers to follow the development of immune system. RAG-1 and IgM mRNA expression was detectable at 5 dphand remained so until 55 dph. These patterns of expression may suggest that the olive flounder start to develop its functionaround 5 dph. Tissue distribution was found that both genes mRNAs are only expressed in the immune-related organ such asspleen, kidney and gill. The early detection of IgM mRNA led to the investigation of its presence in oocytes. Both RAG-1 andIgM mRNA transcripts were detected in unfertilized oocytes, suggesting that they are maternally transferred. The biologicalsignificance of such a phenomenon remains to be investigated.
참고문헌 (30)
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