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CT105로 유도된 신경모세포종 세포주에서 指迷湯의 항치매효과

Effects on Alzheimer's disease by Jimitang in CT105-overexpressed SK-N-SH cell lines

박치상|강승준|박창국
대한한방내과학회 (The Society Of Korean Oriental Internal Medicine)|2004.09|대한한방내과학회지 (The Journal of Korean Oriental Internal Medicine)|제25권 제3호|pp. 482-491

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Abstract

Alzheimer's disease(AD) is a geriatric dementia that is widespread in old age. In the near future AD may be the biggest problem in public health service. Although a variety of oriental therapies in the study of Jimitang have been traditionally utilized for the treatment of AD, their pharmacological effects and active mechanisms have not been fully elucidated. This study is an investigation of effects of Jimitang on apoptotic cell death induced by CT105 overexpression in SK-N-SH neuroblastoma cell lines DNA fragmentation, neurite outgrowth assay and LDH activity assay were examined. The regeneratory and inhibitory effects on Alzheimer's disease in pCT105-induced neuroblastoma cell lines by Jimitang water extract were examined. Findings from these experiments have shown that Jimitang inhibits the synthesis or activities of CT105, which has neurotoxicities and apoptotic activities in cell lines. In addition, pretreatment of Jimitang(>50 ㎍/mL for 12 hours) partially prevented CT(105)-induced cytotoxicity in SK-N-SH cell lines, and were inhibited by pretreatment. Jimitang(>50 ㎍/mL for 12 hours) repaired CT(105)-induced neurite outgrowth when SK-N-SH cell lines were transfected with CT(105). Results of this study show that, in the Jimitang group, the apoptosis in the nervous system is inhibited, the repair against the degerneration of neuroblastoma cells by CT105 expression is promoted. In addition, Jimitang was found to inhibit DNA fragmentation induced by CT105 overexpression, and promote neurite outgrowth. These findings suggest that Jimitang is beneficial for the treatment of AD.

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관련 논문AI추천

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