Anti-oxidative Properties of Lipids Extracted from Rainbow Trout (Oncorhynchus mykiss) Fed with Carotenoids and Conjugated Linoleic Acid
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A commercial diet supplemented with carotenoids and conjugated linoleic acid was fed to rainbow trout (Oncorhynchus mykiss) for 8 weeks. To investigate the anti-oxidative properties of these compounds, lipids from the muscle and viscera of the fish were subjected to different assays. At 10 μg/mL, L-ascorbic acid exhibited 95% 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity, while the tissue lipids showed little radical scavenging activity. At 50 and 100 μg/mL, the lipids of the muscles and viscera showed 11.7-22.6% and 11.3-24.9% DPPH radical-scavenging activity, respectively. A lipid peroxidation inhibitory assay using the ferric thiocyanate method was also performed in comparison with α-tocopherol at a concentration of 6.0 mg/mL. Our results indicate that the anti-oxidative property of the lipids in fish muscle, which was 85.2% compared to 85.3% for the visceral lipids, was stronger than that of α-tocopherol (74.3%) following 3 days of storage at 40°C.
Abstract
A commercial diet supplemented with carotenoids and conjugated linoleic acid was fed to rainbow trout (Oncorhynchus mykiss) for 8 weeks. To investigate the anti-oxidative properties of these compounds, lipids from the muscle and viscera of the fish were subjected to different assays. At 10 μg/mL, L-ascorbic acid exhibited 95% 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity, while the tissue lipids showed little radical scavenging activity. At 50 and 100 μg/mL, the lipids of the muscles and viscera showed 11.7-22.6% and 11.3-24.9% DPPH radical-scavenging activity, respectively. A lipid peroxidation inhibitory assay using the ferric thiocyanate method was also performed in comparison with α-tocopherol at a concentration of 6.0 mg/mL. Our results indicate that the anti-oxidative property of the lipids in fish muscle, which was 85.2% compared to 85.3% for the visceral lipids, was stronger than that of α-tocopherol (74.3%) following 3 days of storage at 40°C.
참고문헌 (31)
- Bandarra NM, "Antioxidant synergy of α-tocopherol and phospholipids", J Am Oil Chem Soc, 76, pp.905~913 (1999)
- Bligh EG, "A rapid method of total lipid extraction and purification", Can J Biochem Physiol, 37, pp.911~917 (1959)
- Burton GW, "β-Carotene: an unusual type of lipid antioxidant", Science, 224, pp.569~573 (1984)
- Büyükçapar HM, "Pigmenta-tion of rainbow trout (Oncorhynchus mykiss) with carotenoids from marigold flower (Tagetes erecta) and red pepper (Capsicum annum)", Turkish J Veter Ani Sci, 31, pp.7~12 (2007)
- Calliste CA, "Free radical scavenging activities measured by electron spin resonance spectroscopy and B 16 cell antiproliferative behaviors of seven plants", J Agric Food Chem, 49, pp.3321~3327 (2001)
- Chen G, "Carotenoids are degraded by free radicals but do not affect lipid peroxidation in unilamellar liposomes under different oxygen tensions", FEBS Letters, 505, pp.151~154 (2001)
- Cho SY, "Autooxidation of ethyl eicosapen-taenoate and docosahexaenoate", J Am Oil Chem Soc, 64, pp.876~879 (1987)
- Choi BD, "Utilization of ascidian (Halocynthia roretzi) tunic 5. Feeding effect of ascidian tunic extracts on liver lipid of rainbow trout, Oncorhynchus mykiss", J Korean Fish Soc, 27, pp.445~453 (1994)
- Choi BD, "Effect of ascidian tunic extracts on cholesterol accumulation and pig-mentation of kuruma prawn, Penaeus japonicus", J Korean Fish Soc, 29, pp.393~408 (1996)
- Chou ST, "Antioxidative activity and safety of 50% ethanolic red bean extract (Phaseolus radiatus L. var. Aurea)", J Food Sci, 68, pp.21~25 (2003)
- Rui Guo, "Effects of Dietary Conjugated Linoleic Acid on Growth Lipid Class, and Fatty Acid Composition in Rainbow Trout (Oncorhynchus mykiss)", Fisheries and Aquatic Sciences, 11(3), pp.125~132 (2008), 한국수산과학회
- Haard NF, "Biochemistry and chemistry of color and color changes in seafood. In: Advances in Seafood Biochemistry, Composition and Quality", pp.305~310 (1992), Publishing Co. Inc., Lancaster and Basel
- Halliwell B, "Role of free radicals and catalytic metal ions in human disease: An overview", Methods Enzymol, 186, pp.1~85 (1990)
- Ip C, "Mammary cancer prevention by conjugated dienoic derivatives of linoleic acid", Cancer Res, 51, pp.6118~6124 (1991)
- Ip MM, "Conjugated linoleic acid inhibits proliferation and induces apoptosis of normal rat mammary epithelial cells in primary culture", Exp Cell Res, 250, pp.22~34 (1999)
- Josyula S, "Inhibition of DNA adducts formation of PhIP in female F344 rats by dietary conjugated linoleic acid", Nutr Cancer, 32, pp.132~138 (1998)
- Kosugi H, "Interpretation of the thiobarbituric acid reactivity of rat liver and brain homogenates in the presence of ferric ion and ethylenediaminetetraacetic acid", Anal Biochem, 202, pp.249~255 (1992)
- Krinsky NI, "Effects of carotenoids in cellular and animal systems", Am J Clin Nutr, 53, pp.238~246 (1991)
- Lee KN, "Conjugated linoleic acid and atherosclerosis in rabbits", Athero-sclerosis, 108, pp.19~25 (1994)
- Mau JL, "Antioxidant pro-perties of several specialty mushrooms", Food Res Int, 35, pp.519~526 (2002)
- Olson JA, "Provitamin A function of carotenoids: the conversion of β-carotene into vitamin A", J Nutr, 119, pp.105~108 (1989)
- Oyaizu M, "Studies on products of browning reaction: Antioxidative activity of products of browning reac-tion prepared from glucosamine", Jpn J Nutr, 44, pp.307~315 (1986)
- Park WS, "Chemical synthesis of conjugated linoleic acid (CLA) derivatives with glycerol", J Korean Soc Food Sci Nutr, 29, pp.389~394 (2000)
- Qian SY, "EPR detection of lipid derived free radicals from PUFA, LDL, and cell oxidations", Free Radic Biol Med, 29, pp.568~579 (2000)
- SAS, "SAS User’s Guide" (1986), SAS Institute Inc., Cary
- Sugano M, "Conjugated linoleic acid modulates tissue levels of chemical mediators and immuno-globulins in rats", Lipids, 33, pp.521~527 (1998)
- Swinburn B, "Obesity prevention in children and adolescents", Child Adol Psychi Clin North America, 18, pp.209~223 (2009)
- Turek JJ, "Modulation of macrophage cytokine production by conjugated linoelic acids in influenced by the dietary n-6:n-3 fatty acid ratio", J Nutr Biochem, 9, pp.258~266 (1998)
- Manssur Yalpani, "New Technologies For Health Foods and Nutraceuticals", Preventive Nutrition and Food Science, 7(2), pp.222~229 (2002), 한국식품영양과학회
- Yurawecz MP, "Furan fatty acids determined as oxidation products of conjugated octadecadienoic acid", Lipids, 30, pp.595~598 (1995)
- Zhang A, "Oxidative stability of con-jugated linoleic acids relative to other polyunsaturated fatty acids", J Am Oil Chem Soc, 74, pp.1611~1613 (1997)