Identification and Characterization of Three Differentially Expressed Ovarian Genes Associated with Ovarian Maturation in Yesso Scallop, Patinopecten yessoensis
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Despite great commercial interest, relatively little has been described about molecular mechanism of bivalve reproduction. We investigated genes involved in ovarian maturation of the Yesso scallop, Patinopecten yessoensis. GSI index and histological analysis revealed that maturation of ovary begin in February and spawning period is from April to June which is similar to the previous study in the East Sea. As result of combination analysis of differential display RT-PCR (DDRT-PCR) and histological examination, vitellogenin (Vg), ferritin (Ft) and ADT/ATP carrier protein (ACC) were identified as differently expressed genes in maturating ovary. End-point RT-PCR results showed that Vg is ovary-specific genes whereas Ft and ACC are expressed ubiquitously suggesting that Vg can be good molecular markers for ovarian development and sex determination in bivalves. Quantitative PCR results revealed that Vg were expressed highest during growth stage and appears to play a major role in oocyte maturation. On the contrary, expression of Ft was highest after spawning stage, which suggests that up-regulation may be involved in spawning and inactive stages in which the scallops recover from spawning. In addition, high level of the mitochondrial gene, ACC, may play a role in energy metabolism in maturating oocytes. Isolation and molecular studies of these key genes will expand our knowledge of the physiological changes from various exogenous factors including temperature, salinity, pH, even or numerous endocrine disrupting chemicals (EDCs) during reproductive cycle. In addition, further study of these genes implicates various industrial applications including the stable seed production, increased food quality, or economic aquaculture system.
Abstract
Despite great commercial interest, relatively little has been described about molecular mechanism of bivalve reproduction. We investigated genes involved in ovarian maturation of the Yesso scallop, Patinopecten yessoensis. GSI index and histological analysis revealed that maturation of ovary begin in February and spawning period is from April to June which is similar to the previous study in the East Sea. As result of combination analysis of differential display RT-PCR (DDRT-PCR) and histological examination, vitellogenin (Vg), ferritin (Ft) and ADT/ATP carrier protein (ACC) were identified as differently expressed genes in maturating ovary. End-point RT-PCR results showed that Vg is ovary-specific genes whereas Ft and ACC are expressed ubiquitously suggesting that Vg can be good molecular markers for ovarian development and sex determination in bivalves. Quantitative PCR results revealed that Vg were expressed highest during growth stage and appears to play a major role in oocyte maturation. On the contrary, expression of Ft was highest after spawning stage, which suggests that up-regulation may be involved in spawning and inactive stages in which the scallops recover from spawning. In addition, high level of the mitochondrial gene, ACC, may play a role in energy metabolism in maturating oocytes. Isolation and molecular studies of these key genes will expand our knowledge of the physiological changes from various exogenous factors including temperature, salinity, pH, even or numerous endocrine disrupting chemicals (EDCs) during reproductive cycle. In addition, further study of these genes implicates various industrial applications including the stable seed production, increased food quality, or economic aquaculture system.
참고문헌 (51)
- Abdelmajid H, "Differences in tyrosine phosphorylation of oocyte key proteins during 5HT-induced meiosis reinitiation in two bivalve species", Exp Cell Res, 212, pp.422~425 (1994)
- Abdu U, "The vitellogenin cDNA of Cherax quadricarinatus encodes a lipoprotein with calcium binding ability, and its expression is induced following the removal of the androgenic gland in a sexually plastic system", Gen Comp Endocrinol, 127, pp.263~272 (2002)
- Beck G, "Evolution of the acute phase response: iron release by echinoderm (Asterias forbesi) coelomocytes, and cloning of an echinoderm ferritin molecule", Dev Comp Immunol, 26, pp.11~26 (2002)
- Boutet I, "Characterization of reproduction-specific genes in a marine bivalve mollusc: influence of maturation stage and sex on mRNA expression", Gene, 407, pp.130~138 (2008)
- Cummins JM, "Fertilization and elimination of the paternal mitochondrial genome", Hum Reprod, 15, pp.92~101 (2000)
- Davis GP, "Integrating molecular genetic technology with traditional approaches for genetic improvement in aquaculture species", Aqua. Res., 31, pp.3~10 (2000)
- Dukeman AK, "The reproductive cycle of the flame scallop, Ctenoides scaber (Born 1778), from the lower Florida keys and its relationship with environmental conditions", J.shell. Res., 24, pp.341~351 (2005)
- FAO, "Fishery Statistics", Aquaculture Production, ~ (2003)
- Fissore RA, "Mechanisms underlying oocyte activation and postovulatory ageing", Reproduction, 124, pp.745~754 (2002)
- Fleury E, "Characterization of a gonad-specific transform-ing growth factor-beta superfamily member diffe-rentially expressed during the reproductive cycle of the oyster Crassostrea gigas", Gene, 410, pp.187~196 (2008)
- Fong PP, "Huminski PT and D'Urso LM. 1998. Induction and potentiation of parturition in fingernail clams (Sphaerium striatinum) by selective serotonin re-uptake inhibitors (SSRIs)", J Exp Zool, 280, pp.260~264 (1998)
- Gagne F, "Evaluation of estrogenic effects of municipal effluents to the freshwater mussel Elliptio complanata", Comp Biochem Physiol C Toxicol Pharmacol, 128, pp.213~225 (2001)
- Gaulejac Bd, "An Ultra-structural study of gametogenesis of the marine bivalve Pinna novilis (LINNAEUS 1758)", oogenesis J. Moll. Stud., 61, pp.375~392 (1995)
- Hirai S, "Induction of spawning and oocyte matura-tion by 5-hydroxytryptamine in the surfclam", J. Exp. Zool., 245, pp.318~321 (1988)
- Hunt PA, "Sex matters in meiosis", Science, 296, pp.2181~2183 (2002)
- Jansen RP, "The bottleneck: mito-chondrial imperatives in oogenesis and ovarian follicular fate", Mol Cell Endocrinol, 145, pp.81~88 (1998)
- Kajiwara M, "Tissue preferential expres-sion of estrogen receptor gene in the marine snail, Thais clavigera", Gen Comp Endocrinol, 148, pp.315~326 (2006)
- Kanda A, "Molecular and functional characterization of a novel gonadotropin-releasing-hormone receptor isolated from the common octopus (Octopus vulgaris)", Bio-chem J, 395, pp.125~135 (2006)
- Keay J, "The Octopus vulgaris estrogen receptor is a constitutive trans-criptional activator: evolutionary and functional im-plications", Endocrinology, 147, pp.3861~3869 (2006)
- Kim HW, "Three calpains and ecdysone receptor in the land crab Gecarcinus lateralis: sequences, expression and effects of elevated ecdysteroid induced by eyestalk ablation", J Exp Biol, 208, pp.3177~3197 (2005)
- Kim KS, "Pancreatic lipase-related protein (PY-PLRP) highly expressed in the vitellogenic ovary of the scallop, Patinopecten yessoensis", Comp Biochem Physiol B Biochem Mol Biol, 151, pp.52~58 (2008)
- Kim YJ, "Annealing control primer system for identification of differentially expressed genes on agarose gels", Bio-techniques, 36, pp.424~426 (2004)
- Klee CB, "Regulation of the calmodulin-stimulated protein phosphatase, calcineu-rin", J Biol Chem, 273, pp.13367~13370 (1998)
- Le NT, "The role of iron in cell cycle progression and the proliferation of neoplastic cells", Biochim Biophys Acta, 1603, pp.31~46 (2002)
- Li Q, "Changes in vitellin during oogenesis and effect of estradiol-17β on vitellogenesis in the Pacific oyster Crassostrea gigas", Invert. Reprod. Dev., 33, pp.87~89 (1998)
- Matsumoto T, "Oyster estrogen receptor: cDNA cloning and immunolocalization", Gen Comp Endocrinol, 151, pp.195~201 (2007)
- Matsumoto T, "Molecular characterization of a cDNA encoding putative vitellogenin from the Pacific oyster Crassostrea gigas", Zoolog Sci, 20, pp.37~42 (2003)
- Matsumoto T, "Ovarian follicle cells are the site of vitellogenin synthesis in the Pacific abalone Haliotis discus hannai", Comp Biochem Physiol A Mol Integr Physiol, 149, pp.293~298 (2008)
- Okuno A, "Deduced primary structure of vitellogenin in the giant fresh-water prawn, Macrobrachium rosenbergii, and yolk processing during ovarian maturation", J Exp Zool, 292, pp.417~429 (2002)
- Osada M, "Molecular cloning and expression analysis of vitello-genin in scallop, Patinopecten yessoensis (Bivalvia, Mollusca)", Mol Reprod Dev, 67, pp.273~281 (2004)
- Osada M, "Estrogen synthesis in relation to gonadal development of Japanese scallop, Patinopecten yessoensis: gonadal profile and immunolocalization of P450 aromatase and estrogen", Comp Biochem Physiol B Biochem Mol Biol, 139, pp.123~128 (2004)
- Petersen-Mahrt SK, "AID mutates E. coli suggesting a DNA deamination mechanism for antibody diversification", Nature, 418, pp.99~103 (2002)
- Ramalho-Santos J, "Mitochondrial functionality in reproduction: from gonads and gametes to embryos and embryonic stem cells", Hum Reprod Update, ~ (2009)
- Raviv S, "Complete sequence of Litopenaeus vannamei (Crustacea: Decapoda) vitellogenin cDNA and its expression in endocrinologically induced sub-adult females", Gen Comp Endocrinol, 145, pp.39~50 (2006)
- Rodet F, "Molecular cloning of a molluscan gonadotropin-releasing hormone receptor orthologue specifically expressed in the gonad", Biochim Biophys Acta, 1730, pp.187~195 (2005)
- Rodet F, "Alternative splicing of a single precursor mRNA generates two subtypes of Gonadotropin-Releasing Hormone receptor orthologues and their variants in the bivalve mollusc Crassostrea gigas", Gene, 414, pp.1~9 (2008)
- Salisbury JL, "Centrin-2 is required for centriole duplication in mammalian cells", Curr Biol, 12, pp.1287~1292 (2002)
- Sappington TW, "Molecular charac-teristics of insect vitellogenins and vitellogenin receptors", Insect Biochem Mol Biol, 28, pp.277~300 (1998)
- Tanabe T, "A novel oocyte maturation arresting factor in the central nervous system of scallops inhibits serotonin-induced oocyte maturation and spawning of bivalve mollusks", Gen Comp Endocrinol, 147, pp.352~361 (2006)
- Thouas GA, "Mitochondrial dysfunction in mouse oocytes results in preimplantation embryo arrest in vitro", Biol Reprod, 71, pp.1936~1942 (2004)
- Tiu SH, "Characterization of vitellogenin in the shrimp Metapenaeus ensis: expression studies and hormonal regulation of MeVg1 transcription in vitro", Mol Reprod Dev, 73, pp.424~436 (2006)
- Tsutsui N, "Molecular Charac-terization of a cDNA Encoding Vitellogenin and Its Expression in the Hepatopancreas and Ovary during Vitellogenesis in the Kuruma Prawn, Penaeus japonicus", Zoolog Sci, 17, pp.651~660 (2000)
- Tsutsui N, "Molecular characterization of a cDNA encoding vitellogenin in the coonstriped shrimp, Pandalus hypsinotus and site of vitellogenin mRNA expression", J Exp Zoolog A Comp Exp Biol, 301, pp.802~814 (2004)
- Uddin MJ, "Comparative reproductive biology of Yezo scallop, Patinopecten yessoensis, under two different culture systems on the east coast of Korea", Aqua-culture, 265, pp.139~147 (2007)
- Uryu M, "Molecular cloning of cDNA encoding two subunits of calcineurin from scallop testis: demon-stration of stage-specific expression during maturation of the testis", J Biochem, 127, pp.739~746 (2000)
- Varaksina GS, "Effects of estradiol, progesterone, and testosterone on oogenesis of yezo scallop", Bio. Mora., 3, pp.61~68 (1991)
- Wallace RA, "Oogenesis in Fundulus heteroclitus. I. Preliminary observations on oocyte maturation in vivo and in vitro", Dev Biol, 62, pp.354~369 (1978)
- Wedekind JE, "Messenger RNA editing in mammals: new members of the APOBEC family seeking roles in the family business", Trends Genet, 19, pp.207~216 (2003)
- Wolfrum U, "Centrin in the photoreceptor cells of mammalian retinae", Cell Motil Cytoskeleton, 32, pp.55~64 (1995)
- Wong H, "The lipase gene family", J Lipid Res, 43, pp.993~999 (2002)
- Yoshikawa K, "AID enzyme-induced hypermutation in an actively transcribed gene in fibroblasts", Science, 296, pp.2033~2036 (2002)