Antioxidant and Cholinesterase Inhibitory Activities of Aqueous Extract from Rainbow Trout Oncorhynchus mykiss
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
We investigated the antioxidant and cholinesterase inhibitory activities of the aqueous extract of rainbow trout Oncorhynchus mykiss. The antioxidant activity of O. mykiss aqueous extract was determined by in vitro peroxynitrite scavenging activity and reducing power assays. The aqueous extract of O. mykiss showed potent peroxynitrite radical scavenging activity (IC_(50)=0.12±0.001 mg/mL) and reducing power (absorbance=0.47±0.001) at the concentration of 1 mg/mL. The in vitro cholinesterase inhibitory activity of O. mykiss aqueous extract was examined using spectrophotometric analyses of acetyl- and butyrylcholinesterase. The aqueous extract of O. mykiss showed acetylcholinesterase inhibitory activity (IC_(50)=1.61±0.13 mg/mL), but did not exhibit inhibitory activity against butyrylcholinesterase. These results suggest that O. mykiss possesses antioxidant and acetylcholinesterase inhibitory activities and provide scientific evidence for the health benefits of O. mykiss aqueous extract.
Abstract
We investigated the antioxidant and cholinesterase inhibitory activities of the aqueous extract of rainbow trout Oncorhynchus mykiss. The antioxidant activity of O. mykiss aqueous extract was determined by in vitro peroxynitrite scavenging activity and reducing power assays. The aqueous extract of O. mykiss showed potent peroxynitrite radical scavenging activity (IC_(50)=0.12±0.001 mg/mL) and reducing power (absorbance=0.47±0.001) at the concentration of 1 mg/mL. The in vitro cholinesterase inhibitory activity of O. mykiss aqueous extract was examined using spectrophotometric analyses of acetyl- and butyrylcholinesterase. The aqueous extract of O. mykiss showed acetylcholinesterase inhibitory activity (IC_(50)=1.61±0.13 mg/mL), but did not exhibit inhibitory activity against butyrylcholinesterase. These results suggest that O. mykiss possesses antioxidant and acetylcholinesterase inhibitory activities and provide scientific evidence for the health benefits of O. mykiss aqueous extract.
참고문헌 (20)
- Association of Official Analytical Chemists, "Official Methods of Analysis.16th ed", pp.49~59 (1995), Association of Official Analytical Chemists
- Atif F, "In vitro free radical scavenging activity of hepatic matallothionein induced in an Indian freshwater fish,Channa punctata Bloch", Chem Biol Interact, 162, pp.172~180 (2006)
- Bachman DL, "Prevalence of dementia and probable senile dementia of the Alzheimer type in the Framingham study", Neurololgy, 42, pp.115~119 (1992)
- Behnke RJ, "Native Trout of Western North America.American Fisheries Society Monograph 6", pp.1~275 (1992), Bethesda
- 최치송, "무지개 송어(Oncorhynchus mykiss)의 영양 성분에 대한 astaxanthin 첨가사료의 영향", 한국수산과학회지, 43(2), pp.109~116 (2010), 한국수산과학회
- Ellman GL, "A new and rapid colorimetric deter-mination of acetylcholinesterase activity", Biochem Pharmacol, 7, pp.88~95 (1961)
- Giacobini E, "Drugs that target cholinesterase.In Cognitive Enhancing Drugs", pp.11~36 (2004), Birkhauser-Verlag
- Kim KS, "Food components of coho salmon and rainbowtrout", Korean J Food Nutr, 6, pp.73~80 (1993)
- Kooy NW, "Peroxynitrite-mediate oxidation of dihydror-hodamine 123", Free Radic Biol Med, 16, pp.146~156 (1994)
- Lim GP, "A diet enriched with omega-3 fatty acid docosahexaenoic acid reduces amyloid burden in an aged Alzheimer mouse model", J Neurosci, 25, pp.3032~3240 (2005)
- Matsuno T, "Comparative biochemical studies of carotenoids in fishes-XIX,carotenoids of chum salmon,coho salmon,biwa trout,red-spotted masu salmon,masu salmon and kokanee", Bull Jpn Soc Sci Fish, 46, pp.879~884 (1980)
- Oh HT, "Antioxidative and antimutagenic activities of 70% ethanol extract from masou salmon (Oncorhynchus masou)", Toxicol In Vitro, 22, pp.1484~1488 (2008)
- Oh HT, "Masou salmon (Oncorhynchus masou) ethanol extract decreases 3-hydroxy-3-methylglutaryl coenzyme A reductase expression in diet-induced obese mice", Nutr Res, 29, pp.123~129 (2009)
- Oyaizu M, "Studies on products of browning reaction:antioxidative activities of products of browning reaction prepared from glucosamine", Jpn J Nutr, 44, pp.307~315 (1986)
- Sastre J, "Mitochondrial oxidative stress plays a key role in aging and apoptosis", IUBMB Life, 49, pp.427~435 (2000)
- Schneider LS, "Treatment of Alzheimer’s disease with cholinesterase inhibitors", Clin Geriatr Med, 17, pp.337~358 (2001)
- Silman I, "Acetylcholinesterase:‘classical’ and ‘non-classical’ functions and pharma-cology", Curr Opin Pharmacol, 5, pp.293~302 (2005)
- Singh N, "Free radical scavenging activity of an aqueous extract of potato peel", Food Chem, 85, pp.611~616 (2004)
- Squadrito GL, "Oxidative chemistry of nitric oxide:the roles of superoxide,peroxynitrite,and carbon dioxide", Free Radic Biol Med, 25, pp.392~403 (1998)
- Yu Q, "Synthesis of novel phenserine-based-selective inhibitors of butyrylcholinesterase for Alzheimer’s disease", J Med Chem, 42, pp.1855~1861 (1999)