본문 바로가기
KERD
논문KCI 등재

Dipeptide (Tyr-Ile) Acting as an Inhibitor of Angiotensin -I-Converting Enzyme (ACE) from the Hydrolysate of Jellyfish Nemopilema nomurai

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류수산학

초록

The jellyfish Nemopilema nomurai was hydrolyzed with papain and a novel dipeptide purified via ultrafiltration, gel filtration chromatography with Sephadex LH-20, and reverse phase chromatography using C_(18) and C_(12) columns. The IR, 1H NMR, 13C NMR, and MS spectrometer analyses showed that the dipeptide comprised tyrosine-isoleucine (Tyr-Ile). The IC_(50) and K_i values were 6.56 ± 1.12 and 3.10 ± 0.28 μM, respectively, indicating competitive inhibition of angiotensin-Ι-converting enzyme (ACE). As a novel ACE-inhibitory active peptide, Tyr-Ile may have potential for use in antihypertensive therapy.

Abstract

The jellyfish Nemopilema nomurai was hydrolyzed with papain and a novel dipeptide purified via ultrafiltration, gel filtration chromatography with Sephadex LH-20, and reverse phase chromatography using C_(18) and C_(12) columns. The IR, 1H NMR, 13C NMR, and MS spectrometer analyses showed that the dipeptide comprised tyrosine-isoleucine (Tyr-Ile). The IC_(50) and K_i values were 6.56 ± 1.12 and 3.10 ± 0.28 μM, respectively, indicating competitive inhibition of angiotensin-Ι-converting enzyme (ACE). As a novel ACE-inhibitory active peptide, Tyr-Ile may have potential for use in antihypertensive therapy.

참고문헌 (28)

  1. Astawan M, "Effects of angiotensin-I converting enzyme inhibitory substances derived from Indonesian dried-salted fish on blood pressure of rats", Biosci Biotechnol Biochem, 59, pp.425~429 (1995)
  2. Byun HG, "Purification and characterization of angiotensin I converting enzyme (ACE) inhibitory peptides from Alaska pollack (Theragra chalcogramma) skin", Process Biochem, 36, pp.1155~1162 (2001)
  3. Cheung HS, "Binding of peptide substrates and inhibitors of angiotensin converting enzyme.Importance of the COOH-terminal dipeptide sequence", J Biol Chem, 255, pp.401~407 (1980)
  4. Cushman DW, "Spectrophotometeric assay and properties of angiotensin-converting enzyme of rabbit lung", Biochem Pharmacol, 20, pp.1637~1648 (1971)
  5. Dziuba J, "Biologically active peptides from plant and animal proteins", Pol J Food Nutr Sci, 8, pp.3~16 (1999)
  6. Erdmann K, "The ACE inhibitory dipeptide Met-Tyr diminishes free radical formation in human endothelial cells via induction of heme oxygenase-1 and ferritin", J Nutr, 136, pp.2148~2152 (2006)
  7. FitzGerald RJ, "The emerging role of dairy protein and bioactive peptides in nutrition and health:hypotensive peptides from milk proteins", J Nutr, 134, pp.980S~988S (2004)
  8. Fujita H, "LKPNM:a prodrug-type ACE-inhibitory peptide derived from fish protein", Immunopharmcology, 44, pp.123~127 (1999)
  9. Gao D, "Angiotensin I-converting enzyme inhibitor derived from cottonseed protein hydrolysate", Afr J Biotechnol, 9, pp.8977~8983 (2010)
  10. Gobbetti M, "Production of angiotensin-I-converting-enzyme-inhibitory peptides in fermented milks started by Lactobacillus delbrueckii subsp.Bulgaricus SS1 and Lactococcus lactis subsp.Cremoris FT4", Appl Environ Microbiol, 66, pp.3898~3904 (2000)
  11. He H, "Preparation and functional evaluation of oligopeptide-enriched hydrolysates from shrimp (Acetes chinesis) treated with crude protease from Bacillus sp. SM98011", Bioresour Technol, 97, pp.385~390 (2006)
  12. Jang A, "Purification and identification of angiotensin converting enzyme inhibitory peptides from beef hydrolysates", Meat Sci, 69, pp.653~661 (2005)
  13. Je JY, "Isolation of angiotensin I converting enzyme (ACE) inhibitor from fermented oyster sauce, Crassostrea gigas", FOOD CHEMISTRY, 90(4), pp.809~814 (2005), ELSEVIER SCI LTD
  14. Kawasaki T, "Antihypertensive effect of valyl-tyrosine,a short chain peptide derived from sardine muscle hydrolyzate,on mild hypertensive subjects", J Hum Hypertens, 14, pp.519~523 (2000)
  15. Kim SK, "Screening of biofunctional peptides from cod processing wastes", J Korean Soc Agric Chem Biotechnol, 43, pp.225~227 (2000)
  16. Kim TJ, "Angiotensin I converting enzyme inhibitory activity of hot-water extract and enzymatic hydrolysate of fresh water fish", J Korean Soc Food Sci Nutr, 25, pp.871~877 (1996)
  17. Kohama Y, "Isolation of angiotensin-converting enzyme inhibitor from tuna muscle", Biochem Biophys Res Commun, 155, pp.332~337 (1988)
  18. Lee HD, "Separation and purification of angiotensin-I converting enzyme inhibitory peptides from laver hydrolysate", J Korean Fish Sci, 34, pp.164~172 (2001)
  19. K. J. Lee, "Separation and Purification of Angiotensin Converting Enzyme Inhibitory Peptides Derived from Goat's Milk Casein Hydrolysates", ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 18(5), pp.741~746 (2005), 아세아·태평양축산학회
  20. Matsui T, "Inhibition of angiotensin I-converting enzyme by Bacillus licheniformis alkaline protease hydrolyzates derived from sardine muscle", Biosci Biotechnol Biochem, 57, pp.922~925 (1993)
  21. Miguel M, "Antihypertensive,ACE-inhibitory and vasodilator properties of egg white hydrolysates:effect of a simulated intestinal digestion", Food Chem, 104, pp.163~168 (2007)
  22. Ondetii MA, "Design of specific inhibitors of angiotensin-converting enzyme:a new class of orally active antihypertensive agents", Science, 196, pp.441~444 (1977)
  23. Shahidi F, "Bioactive peptides", J AOAC Int, 91, pp.914~931 (2008)
  24. Smacchi E, "Peptides from several Italian cheeses inhibitory to proteolytic enzymes of lactic acid bacteria,Pseudomonas fluorescens ATCC 948 and to the angiotensin I-converting enzyme:design,synthesis,and utilization", Enzyme Microb Technol, 22, pp.687~694 (1998)
  25. Sugiyama K, "Hypotensive effect of fish protein hydrolysate", Nippon Nogeikagaku Kaishi, 65, pp.35~43 (1991)
  26. Taylor WH, "Formol titration:an evaluation of its various modifications", Analyst, 82, pp.488~498 (1957)
  27. Vermeirssen V, "Bioavailability of angiotensin I converting enzyme inhibitory peptides", Br J Nutr, 92, pp.357~366 (2004)
  28. Wu H, "Purification and identification of novel angiotensin-I-converting enzyme inhibitory peptides from shark meat hydrolysate", Process Biochem, 43, pp.457~461 (2008)

관련 논문AI추천

ACE-Inhibitory Properties of Proteolytic Hydrolysates from Giant Jellyfish Nemopilema nomurai김연계, 문호성, 박희연, 윤나영, 윤소미, 윤호동, 이두석, 이문희, 임치원한국수산과학회 · Fisheries and Aquatic Sciences2011 · 제14권 제3호The Novel Angiotensin I Converting Enzyme Inhibitory Peptide from Rainbow Trout Muscle Hydrolysate김성래, 변희국한국수산과학회 · Fisheries and Aquatic Sciences2012 · 제15권 제3호오징어 (Todarodes pacificus) 껍질로부터 Angiotensin I 전환효소 저해 펩티드의 분리 정제변희국, 이정권, 전중균한국수산과학회 · 한국수산과학회지2011 · 제44권 제2호Antioxidant and Angiotensin I Converting Enzyme Inhibitory Activities of Red Snow Crab Chionoecetes japonicas Shell Hydrolysate by Enzymatic Hydrolysis김상보, 심길보, 윤나영, 임치원한국수산과학회 · Fisheries and Aquatic Sciences2013 · 제16권 제4호Antioxidative and angiotensin I-converting enzyme inhibitory activities of Eastern eel (Anguilla japonica)김미령, 박선미, 박준호, 최재석, 표상은, 아샤그리 깁탄, 베틀레헴 아베베한국식품저장유통학회 · Food Science and Preservation2020 · 제27권 제7호Isolation and identification of angiotensin I-converting enzyme inhibitory peptides derived from thermolysin-injected beef M. longissimus김현진, 설국환, 이무하, 조철훈, 최주희, 황진택아세아·태평양축산학회 · Animal Bioscience2019 · 제32권 제3호

이 간행물의 다른 논문 (27건)