본문 바로가기
KERD
논문KCI 등재

오징어 (Todarodes pacificus) 껍질로부터 Angiotensin I 전환효소 저해 펩티드의 분리 정제

Purification of Angiotensin I-Converting Enzyme Inhibitory Peptide from Squid Todarodes pacificus Skin

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류수산학

초록

In this study, an angiotensin I-converting enzyme (ACE) inhibitor from squid skin was purified and characterized. Squid (Todarodes pacificus) skin protein isolates were hydrolyzed using six commercial proteases: alcalase, α-chymotrypsin, neutrase, papain, pepsin, and trypsin. The peptic hydrolysate had the highest ACE inhibitory activity. The ACE inhibitory peptide was purified using Sephadex G-25 column chromatography and reverse phase high-performance liquid chromatography (HPLC) with a C_(18) column. The purified ACE inhibitory peptide was identified and sequenced, and found to consist of seven amino acid residues: Ser-Ala-Gly-Ser-Leu-Val-Pro (657Da). The IC_(50) value of the purified ACE inhibitory peptide was 766.2 μM, and Lineweaver-Burk plots suggested that the purified peptide acts as a noncompetitive ACE inhibitor. These results suggest that the ACE inhibitory peptide purified from the peptic hydrolysate of squid skin may be of benefit in developing antihypertensive drugs and functional foods.

Abstract

In this study, an angiotensin I-converting enzyme (ACE) inhibitor from squid skin was purified and characterized. Squid (Todarodes pacificus) skin protein isolates were hydrolyzed using six commercial proteases: alcalase, α-chymotrypsin, neutrase, papain, pepsin, and trypsin. The peptic hydrolysate had the highest ACE inhibitory activity. The ACE inhibitory peptide was purified using Sephadex G-25 column chromatography and reverse phase high-performance liquid chromatography (HPLC) with a C_(18) column. The purified ACE inhibitory peptide was identified and sequenced, and found to consist of seven amino acid residues: Ser-Ala-Gly-Ser-Leu-Val-Pro (657Da). The IC_(50) value of the purified ACE inhibitory peptide was 766.2 μM, and Lineweaver-Burk plots suggested that the purified peptide acts as a noncompetitive ACE inhibitor. These results suggest that the ACE inhibitory peptide purified from the peptic hydrolysate of squid skin may be of benefit in developing antihypertensive drugs and functional foods.

참고문헌 (39)

  1. Ali B, "Angiotensin I-converting enzyme (ACE) inhibitory activities of sardinelle (Sardinella aurita) by-products protein hydrolysates obtained by treatment with microbial and visceral fish serine proteases", J Agr Food Chem, 111, pp.350~356 (2008)
  2. Andrews PR, "Conformational analysis and active site modeling of angiotensin converting enzyme inhibitors", J Med Chem, 28, pp.393~399 (1985)
  3. AOAC, "Official Methods of Analysis. 15th ed" (1990), Association of Official Analytical Chemists
  4. Axena PR, "Interaction between the renin-a ngiotensin aldosterone and sympathetic nervous systems", J Cardiovasc Pharm, 9, pp.80~88 (1992)
  5. Balti R, "Three novel angiotensin I-converting enzyme (ACE) inhibitory peptides from cuttlefish (Sepia officinalis) using digestive proteases", Food Res Int, 43, pp.1136~1143 (2010)
  6. Berstad A, "Gastroenterol", pp.31~38 (1970), University of California
  7. Cheung HS, "Binding of peptide substrates and inhibitors of angiotensin-Iconverting enzyme", J Biol Chem, 255, pp.401~407 (1980)
  8. Cho SH, "Nutritional composition and microflora of the fresh and fermented skate (Raja Kenojei) skins", International J Food Sci Nutri, 55, pp.45~51 (2004)
  9. Cushman DW, "Spectrophotometric assay and properties of the angiotensin I-converting enzyme of rabbit lung", Biochem. Pharmacol, 20, pp.1637~1648 (1971)
  10. Cushman DW, "Development of specific inhibitors of angiotensin I converting enzyme (kininase II)", Fed Proced, 38, pp.2778~2784 (1979)
  11. Do JR, "Separation and purification of angiotensin I-converting enzyme inhibitory peptide from mackerel", J Korean Fish Soc, 33, pp.153~157 (2000)
  12. 도정룡, "어육단백질로부터 분리된 항고혈압 펩타이드가 ACE 저해활성과본태성 고혈압쥐의 혈압에 미치는 영향", 한국식품과학회지, 38(4), pp.567~570 (2006), 한국식품과학회
  13. Engel Sl, "Inhibition of pressure effects of angiotensin I and augmentation of pressure effects of bradykinin by synthetic peptides", Proc Soc Exp Biol Med, 140, pp.240~245 (1972)
  14. Gime´nez B, "Improvement of the antioxidant properties of squid skin gelatin films by the addition of hydrolysates from squid gelatin", Food Hydrocolloid, 23, pp.1322~1327 (2009)
  15. Gomez-Guillen MC, "Structural and physical properties of gelatin extracted form different marine species: a comparative study", Food Hydrocolloid, 16, pp.25~34 (2002)
  16. I-Chuan S, "Isolation and characterisation of a novel angiotensin I-converting enzyme (ACE) inhibitory peptide from the algae protein waste", J Agr Food Chem, 115, pp.279~288 (2009)
  17. Janeke G, "Role of taurine accumulation in keratinocyte hydration", J Invest Dermatol, 121, pp.354~361 (2003)
  18. Je JY, "Purification and characterization of an antioxidant peptide obtained from tuna backbone protein by enzymatic hydrolysis", PROCESS BIOCHEMISTRY, 42(5), pp.840~846 (2007), ELSEVIER SCI LTD
  19. Jeong S.H., "Angiotensin converting enzyme and its inhibitors", Medicine Information, 9, pp.152~158 (1994)
  20. Jianping W, "Production of angiotensin I converting enzyme inhibitory peptides from defatted canola meal", Bioresource Technol, 100, pp.5283~5287 (2009)
  21. Kato H, "Bradykinin-potentiating peptides from the venom of Agkistrodon halys blomhoffii", Experientia, 25, pp.694~695 (1969)
  22. Kim JS, "Screening for the raw material of gelatin from the skin of some pelagic fishes and squid", J Korean Fish Soc, 31, pp.55~61 (1997)
  23. Kim SY, "Functional properties of proteolytic enzyme modified soy protein isolate", J Agric Food Chem, 38, pp.651~656 (1990)
  24. Kim TJ, "Angiotensin Ⅰ converting enzyme inhibitory activity of hot-water extract and enzymatic hydrolysate of fresh water fish", J. Korean Soc. Food Sci Nutri, 25, pp.871~877 (1996)
  25. Kristinsson HG, "Fish protein hydrolysates and their potential use in the food industry", Recent Adv Mar Biotechnol, 7, pp.157~181 (2002)
  26. Lee KS, "Effects of Diet Supplimented with Squid Intestine on Growth and Body Composition of the Catfish (Parasilurus asotus)", J Korean Fish Soc, 31, pp.31~36 (1998)
  27. 천충길, "A novel angiotensin I converting enzyme inhibitory peptide from tuna frame protein hydrolysate and its antihypertensive effect in spontaneously hypertensive rats", FOOD CHEMISTRY, 118(1), pp.96~102
  28. Lowry OH, "Protein measurement with the Folin-Phenol reagent", J Biol Chem, 193, pp.265~275 (1951)
  29. Miyoshi S, "Structures and activity of angiotensin converting enzyme inhibitor in an α-zein hydrosate", Agric Bial Chem, 55, pp.1313~1318 (1991)
  30. 오광석, "옥수수 글루텐 효소 가수분해물의 Angiotensin I Converting Enzyme 활성 저해 펩타이드의 정제", 한국미생물·생명공학회지, 31(1), pp.51~56 (2003), 한국미생물·생명공학회
  31. Ondetti MA, "Enzyme of the renin-angiotensin system and their inhibitors", Ann Rev Biochem, 51, pp.283~308 (1982)
  32. Ondetti MA, "Design of specific inhibitors of angiotensin", Science, 196, pp.441~444 (1977)
  33. Pihlanto L, "Bioactive peptides derived from bovine whey proteins: Opioid and ACE inhibitory", Trends Food Sci Tech, 11, pp.347~356 (2000)
  34. Rajapakse N, "Purification and in vitro antioxidative effects of giant squid muscle peptides on free radical-mediated oxidative systems", J. Nutri Biochem, 16, pp.562~569 (2005)
  35. Ryu IW, "Inhibition effect of ACE (angiotensin converting enzyme) and kinetics of aloe acethyl mannan", Korean J Food Sci Technol, 29, pp.1269~1274 (1997)
  36. Teipel JW, "The number of substrate-and inhibitor-binding sites of fumarase", J Biol Chem, 21, pp.5679~5683 (1968)
  37. Tharanathan RN, "Biodegradable films and composite coatings: past, present and future", Trend. Food Sci Technol, 14, pp.71~78 (2003)
  38. Wang SL, "Conversion of squid pen by Serratia ureilytica for the production of enzymes and antioxidants", Bioresource Technol, 100, pp.316~323 (2009)
  39. Yoshiyuki S, "Structure and activity of angiotensin I converting enzyme inhibitory peptides from sake and sake lees", Biosci Biotech Biochem, 58, pp.1767~1770 (1994)

관련 논문AI추천

The Novel Angiotensin I Converting Enzyme Inhibitory Peptide from Rainbow Trout Muscle Hydrolysate김성래, 변희국한국수산과학회 · Fisheries and Aquatic Sciences2012 · 제15권 제3호살 오징어(Todarodes pacificus) 간췌장 유래 Aminopeptidase 활성획분에 의해 쓴맛이 개선된 멸치 조미소스의 제조 및 식품특성강상인, 권인상, 김진수, 손숙경, 윤인성, 이지운, 최유리, 허민수한국수산과학회 · 한국수산과학회지2021 · 제54권 제6호ACE-Inhibitory Properties of Proteolytic Hydrolysates from Giant Jellyfish Nemopilema nomurai김연계, 문호성, 박희연, 윤나영, 윤소미, 윤호동, 이두석, 이문희, 임치원한국수산과학회 · Fisheries and Aquatic Sciences2011 · 제14권 제3호Antioxidative and angiotensin I-converting enzyme inhibitory activities of Eastern eel (Anguilla japonica)김미령, 박선미, 박준호, 최재석, 표상은, 아샤그리 깁탄, 베틀레헴 아베베한국식품저장유통학회 · Food Science and Preservation2020 · 제27권 제7호Fractionation and Characterization of Fractions with High Antioxidative Activity from the Gelatin Hydrolysates of Korean Rockfish Sebastes schlegelii Skin김진수, 김형준, 박권현, 신준호, 이동호, 이지선, 허민수한국수산과학회 · Fisheries and Aquatic Sciences2011 · 제14권 제3호Antioxidant and angiotensin I-converting enzyme inhibitory activities of northern shrimp (Pandalus borealis) by-products hydrolysate by enzymatic hydrolysis김상보, 심길보, 윤나영, 임치원한국수산과학회 · Fisheries and Aquatic Sciences2016 · 제19권 제3호

이 간행물의 다른 논문 (10건)