서해안에서 분리한 암피실린 내성 비브리오속 세균의 특성
Characteristics of Ampicillin-Resistant Vibrio spp. Isolated from a West Coastal Area of Korean Peninsula
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Abstract
Thirty-eight Vibrio spp. were isolated from the sea waters harvested from the 22 stations located on the west coast of the Korean peninsula in September 2006. The isolates consisted of V. parahaemolyticus (n=21), V. alginolyticus (n=16) and V. cholerae non-01 (n=1), among which 35 isolates displayed resistance against two of the tested antibiotics. Among the 38 isolates, 18 isolates exhibited multi-drug resistance against more than four 4 antibiotics. In particular, minimum inhibitory concentration (MIC)50 and MIC90 of ampicillin-resistant isolates were as high as 2,048μg･mL-1 and 4,096μg･mL-1 respectively. β-lactamase production was examined to analyze the ampicillin-resistance. Some Vibrio spp. isolates produced β-lactamase, however antibiotics resistance pattern and β-lactamase production were not clearly related to each other. A genetic relationship between resistance and gene expression was confirmed in the ampicillin-resistant isolates.
참고문헌 (18)
- Bauer, A.W, "Antibiotic susceptibility testing by standardized single disk method", Am. J. Clin. Pathol, 45, pp.493~496 (1966)
- Ceccarelli, D, "New cluster pf plasmid-located class 1 integrons in Vibrio cholerae 01 and a dfrA15 cassette-containing integron in Vibrio parahaemolyticus isolated in Angola", Antimicrob. Agents Chemother, 50, pp.2493~2499 (2006)
- Clinial Laboratory Standard Institute, "Performance standards for antimicrobial susceptibility testing: 7th informational supplement", M100-S17, Wayne, PA., ~ (2007)
- Food and Drug Administration(FDA), "2005. Quantitative risk assessment on the public health impact of pathogenic Vibrio parahaemolyticus in raw oysters", U.S. Food and Drug Administration, ~ (2005)
- Lee, S.K.Y, "Analysis of the 16S-23S rDNA intergenic spacers (IGSs) of marine vibriosis for species-specific signature DNA sequences", Mar. Pollut. Bull, 44, pp.412~420 (2002)
- Melano, R, "New carbenicillin-hydrolyzing β-lactamase (CARB-7) from Vibrio cholerae non-01, non-0139 strains encoded by the VCR region of the V. cholerae genome", Antimicrob. Agents Chemother, 46, pp.2162~2168 (2002)
- Melian, L, "Intraspecific diversity of Vibrio vulnificus in Galveston Bay Water and oysters as determined by RAPD PCR", Appl. Environ. Microbiol, 69, pp.3170~3175 (2003)
- Mudryk,Z.J, "Occurrence and distribution antibiotic resistance of heterotrophic bacteria isolated from a marine beach", Mar. Pollut. Bull, 50, pp.80~86 (2005)
- Panicker, G, "Detection of pathogenic Vibrio spp. in shellfish by using multiplex PCR and DNA microarrays", Appl. Environ. Microbiol, 70, pp.7436~7444 (2004)
- Petroni, A, "Plasmidic ex- tended-spectrum β-lactamase in Vibrio cholerae 01 E1 tor isolates in Argentina", Antimicro. Agents Chemother, 46, pp.1462~1468 (2002)
- Rowe-Magnus, D.A, "The role of integrons in antibiotic resistance gene capture", Int. J. Med. Microbial, 292, pp.115~125
- Ripabelli, G, "Molecularcharacterisation and anti- microbial resistance of Vibrio vulnificus and Vibrio alginolyticus isolated from mussels (Mytilus galloprovincialis)", Syst. Appl. Microbiol, 26, pp.119~126 (2003)
- 손광태, "남해안 어류양식장에서 분리된 Vibrio parahaemolyticus와 Vibrio alginolyticus의 항균제 감수성", 한국수산과학회지, 38(6), pp.365~371 (2005), 한국수산과학회
- 손진창, "경북 동해안 환경에서 분리된 V. parahaemolyticus 및 V. vulnificus의 생태학적 및 항생제 감수성 특성", 한국환경보건학회지, 29(2), pp.94~102 (2003), 한국환경보건학회
- Su, Y.C, "Vibrio parahaemolyticus: A concern of seafood safety", Food Microbiol, 24, pp.549~558 (2007)
- Teo, J.W.P, "Novel β- lactamase genes from two environmental isolates of Vibrio harveyi", Antimicrob. Agents Chemother, 44, pp.1309~1314 (2000)
- Vaseeharan, B, "In vitro susceptibility of antibiotics against Vibrio spp. and Aeromonas spp. isolated from Penaeus monodon hatcheries and ponds", Int. J. Antimicrob. Agents, 26, pp.285~291 (2005)
- Zanetti, S, "In vitro susceptibility of Vibrio spp. isolated from the environment", Int. J. Antimicrob. Agents, 17, pp.407~409 (2001)