茵蔯清肝汤이 간성상세포의 섬유화 억제에 미치는 영향
Inhibitory Effect of Injinchunggan-tang(Yinchenqinggan-tang) on Fibrogenesis of Rat Hepatic Stellate Cells
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Objectives : This study was performed to investigate the anti-fibrogenic effect of Iinjinchunggan-tang on cultured rat hepatic stellate cells. Materials and Methods : Hepatic Stellate Cells(HSC-T6) were treated with various concentrations of Iinjinchenggang-tang extract for 24, 48, 72 hours. The extraction was done with distilled water. After the treatment, cell viability, proliferation, procollagen levels and the mRNA of the TIMP-1, TIMP-2, ASMA were measured by using MTT Assay, BrdU Assay, Procollagen Type I C-peptide EIA kit, and RT-PCR. Results : The proliferation, mRNA expression and synthesis of collagen of the hepatic stellate cells were inhibited by Injinchunggan-tang treatment with dose-dependent manner. Which indicates the prescription has inhibitory effect on fibrogenesis of the liver by regulating the fibrogenesis associated genes in transcription. Cell viability was inhibited with time- and dose-dependent manners. It seemed that the drug should be used with enough dose to acquire treatment effect. Conclusion : These results suggest that Iinjinchunggan-tang is beneficial in the treatment of cirrhotic patients as well as for the patients with chronic hepatitis.
Abstract
Objectives : This study was performed to investigate the anti-fibrogenic effect of Iinjinchunggan-tang on cultured rat hepatic stellate cells. Materials and Methods : Hepatic Stellate Cells(HSC-T6) were treated with various concentrations of Iinjinchenggang-tang extract for 24, 48, 72 hours. The extraction was done with distilled water. After the treatment, cell viability, proliferation, procollagen levels and the mRNA of the TIMP-1, TIMP-2, ASMA were measured by using MTT Assay, BrdU Assay, Procollagen Type I C-peptide EIA kit, and RT-PCR. Results : The proliferation, mRNA expression and synthesis of collagen of the hepatic stellate cells were inhibited by Injinchunggan-tang treatment with dose-dependent manner. Which indicates the prescription has inhibitory effect on fibrogenesis of the liver by regulating the fibrogenesis associated genes in transcription. Cell viability was inhibited with time- and dose-dependent manners. It seemed that the drug should be used with enough dose to acquire treatment effect. Conclusion : These results suggest that Iinjinchunggan-tang is beneficial in the treatment of cirrhotic patients as well as for the patients with chronic hepatitis.
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