茵蔯五苓散加三七根이 흰쥐의 간섬유화 억제에 미치는 영향
Inhibiting Effect of Injinoryung-san-Ga-Samchilgun on Liver Fibrosis in Rats
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
The aim of this study was to investigate that Injinoryung-san-Ga-Samchilgun(IJORS) has an inhibitory effect on the development of liver fibrosis in rats. The influence of IJORS on liver stellate cell viability in rat was measured by the MTT assay, and proliferation was measured by the BrdU assay. The mRNA expression of procollagen type 1α2, α-SMA, TIMP1, and TIMP2 all of which are associated with liver fibrosis, were analyzed by RT-PCR. The inhibitory effect of IJORS on procollagen production in hepatic stellate cell was examined using by enzyme immuno assay(procollagen Type 1 C-Peptide EIA). And after IJORS was orally administered to experimental rats with thioacetamide(TAA)-induced liver fibrosis for 4 weeks, the body weight, liver function test, complete blood and the change of portal pressure were measured. IJORS prevented hepatic stellate cell viability and proliferation in a dose-dependent manner. IJORS reduced the mRNA expression of procollagen type 1α2, α-SMA and TIMP1 and the production of procollagen protein. IJORS inhibited the increase of AST, ALT, WBC and portal pressure in rats administered by TAA. IJORS is considered to prevent liver fibrosis by inhibiting the activation of stellate cell and production of procollagen and prevent the progress of liver fibrosis by inhibiting the inflammation of liver tissue complicated in many liver disease.
Abstract
The aim of this study was to investigate that Injinoryung-san-Ga-Samchilgun(IJORS) has an inhibitory effect on the development of liver fibrosis in rats. The influence of IJORS on liver stellate cell viability in rat was measured by the MTT assay, and proliferation was measured by the BrdU assay. The mRNA expression of procollagen type 1α2, α-SMA, TIMP1, and TIMP2 all of which are associated with liver fibrosis, were analyzed by RT-PCR. The inhibitory effect of IJORS on procollagen production in hepatic stellate cell was examined using by enzyme immuno assay(procollagen Type 1 C-Peptide EIA). And after IJORS was orally administered to experimental rats with thioacetamide(TAA)-induced liver fibrosis for 4 weeks, the body weight, liver function test, complete blood and the change of portal pressure were measured. IJORS prevented hepatic stellate cell viability and proliferation in a dose-dependent manner. IJORS reduced the mRNA expression of procollagen type 1α2, α-SMA and TIMP1 and the production of procollagen protein. IJORS inhibited the increase of AST, ALT, WBC and portal pressure in rats administered by TAA. IJORS is considered to prevent liver fibrosis by inhibiting the activation of stellate cell and production of procollagen and prevent the progress of liver fibrosis by inhibiting the inflammation of liver tissue complicated in many liver disease.
참고문헌 (22)
- 이문호, "내과학(上)", pp.1015~ (1986), 학문사
- Ramzi, S, "Pathologic Basis of Disease", pp.834~83 (1992), Philadelphia: W.B. Saunders
- 전국한의과대학 간계내과학교수, "간계내과학", pp.323~355 (2001), 동양의학연구원
- 대한가정의학회, "가정의학 임상편", pp.993~999 (2002), 계축문화사
- 禹弘楨, "茵蔯五苓散과 茵蔯增量한 構成方이 흰쥐 肝損傷에 미치는 影響", 대한한의학회지, 113(1), pp.234~241 (1992)
- 안덕균, "原色 韓國本草圖鑑", pp.523~ (2002), (주)교학사
- Won-Hwan Park, "A Korean herbal medicine, Panax notoginseng, prevents liver fibrosis and hepatic microvascular dysfunction in rats", Life Sciences, 76(15), pp.1675~1690 (2005)
- Liu Jie, "The effect of Chinese hepatoprotective medicines on experimental liver injury in mice", Journal of Ethnopharmacology, 42(3), pp.183~191 (1994)
- 통계청, "사망원인통계연보", ~ (2006)
- Iredale, J.P, "Hepatocyte-matrix interactions", Gut, 35, pp.729~732 (1994)
- Isao Sakaida, "Herbal medicine Sho-saiko-to(TJ-9)prevent liver fibrosis and enzyme-altered lesions in rat liver cirrhosis induced by a choline-deficient-amino acid-defined diet", Journal of Hepatology, 28(2), pp.298~306 (1998)
- 김영철, "인진이 간성상세포의 섬유화 억제에 미치는 영향에 대한 연구", 대한한방내과학회지, 26(4), pp.853~863 (2005), 대한한방내과학회
- 심재옥, "인진청간탕이 TGF-β 매개성 간섬유화에 미치는 영향", 대한한의학회지, 24(2), pp.1~11 (2003), 대한한의학회
- 강중원, "五苓散加減方이 3T3-L1 adipocyte의 leptin 및 leptin receptor 함량과 differentiation에 미치는 영향", 대한한의학회지, 26(2), pp.241~251 (2005), 대한한의학회
- 김호현, "茵蔯五苓散이 흰쥐의 腎毒性에 미치는 影響", 대한한의학회지, 17(2), pp.133~144 (1996)
- Iredale, J.P, "Mechanisms of spontaneous resoluyion of rat liver fibrosis. Hepatic stellate cell apoptosis and reduced hepatic expression of metalloproteinase inhibitors", J Clin Invest, 102, pp.538~549 (1998)
- A. Castilla, "Transforming growth factors β1 and α in chronic liver diseases", N Engl J Med, 324, pp.933~940 (1991)
- Benyon, R.C, "Progelatinase A is produced and activated by rat hepatic stellate cells and promotes their proliferation", Hepatology, 30, pp.977~986 (1999)
- Muller, A, "Thioacetamide-induced cirrhosis-like liver lesions in rats usefulness and reliability of this animal model", Exp Pathol, 34, pp.229~236 (1988)
- Rockey, D.C, "Endothelium induced contractility of stellate cells from normal and cirrhotic rat liver: implication for regulation of portal pressure and resistance", Hepatology, 24, pp.233~240 (1996)
- Rockey, D.C, "Activation-dependent contractility of rat hepatic lipocytes in culture and in vivo", J Clin Invest, 92, pp.1795~1804 (1993)
- Lee, J.S, "Mechanisms of nitric oxide interplay with Rho GTPase family members in modulation of actin membrane dynamics in pericytes and fibroblast", AM J Pathol, 166, pp.1861~1870 (2005)