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1-methyl-4-phenylpyridinium(MPP+)로 유도된 파킨슨병의 세포 손상에 대한 황백의 신경세포 보호효과

Neuroprotective Effect of Methanol Extract of Phellodendri Cortex Against 1-methyl-4- Phenylpyridinium-induced Apoptosis in PC-12 Cells

서운교|정혜미|정영석
대한한방내과학회 (The Society Of Korean Oriental Internal Medicine)|2009.03|대한한방내과학회지 (The Journal of Korean Oriental Internal Medicine)|제30권 제1호|pp. 51-63

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키워드

Parkinson's diseasePhellodendri CortexPC12 CellMPP⁺
주제분류한의학

초록

Background and Objective : The prospects for developing an anti-apoptotic natural component or a compound that exerts a neuroprotective effect with few or no side effects for the treatment of neurodegenerative disease appear favorable. In the present study, we evaluated the effects of the methanol extract of Phellodendri Cortex (PC extract) on 1-methyl-4-phenyl pyridinium(MPP+)-induced apoptosis in PC-12 cells. Materials and Metohds : We used the methanol extract of Phellodendri Cortex (PC extract). PC-12 cells was cultured by RPMZ-1640. We found the PC extract's gene expression(bax, bcl-s) by using RT-PCR. And we examined the PC extract's protein expression(bcl-2, bax, cytochrome c, poly(ADP-ribose) polymerase(PARP), caspase-3) by SDS-PAGE and Western blot. Results : Apoptosis in MPP+-induced PC-12 cells was accompanied by an increased bax/bcl-2 ratio, release of cytochrome c to the cytosol and the activation of caspase-3. PC extract inhibited the down-regulation of bcl-2 and the up-regulation of bax, as well as the release of mitochondrial cytochrome c into the cytosol. In addition, PC extract attenuated caspase-3 activation and cleavage of poly (ADP-ribose) polymerase (PARP). Conclusion : These results suggest that the neuroprotective potentials of PC extract against MPP+-induced apoptosis can be, at least partially, ascribed to its anti-apoptotic effects in PC-12cells.

Abstract

Background and Objective : The prospects for developing an anti-apoptotic natural component or a compound that exerts a neuroprotective effect with few or no side effects for the treatment of neurodegenerative disease appear favorable. In the present study, we evaluated the effects of the methanol extract of Phellodendri Cortex (PC extract) on 1-methyl-4-phenyl pyridinium(MPP+)-induced apoptosis in PC-12 cells. Materials and Metohds : We used the methanol extract of Phellodendri Cortex (PC extract). PC-12 cells was cultured by RPMZ-1640. We found the PC extract's gene expression(bax, bcl-s) by using RT-PCR. And we examined the PC extract's protein expression(bcl-2, bax, cytochrome c, poly(ADP-ribose) polymerase(PARP), caspase-3) by SDS-PAGE and Western blot. Results : Apoptosis in MPP+-induced PC-12 cells was accompanied by an increased bax/bcl-2 ratio, release of cytochrome c to the cytosol and the activation of caspase-3. PC extract inhibited the down-regulation of bcl-2 and the up-regulation of bax, as well as the release of mitochondrial cytochrome c into the cytosol. In addition, PC extract attenuated caspase-3 activation and cleavage of poly (ADP-ribose) polymerase (PARP). Conclusion : These results suggest that the neuroprotective potentials of PC extract against MPP+-induced apoptosis can be, at least partially, ascribed to its anti-apoptotic effects in PC-12cells.

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