미성숙 진귤 과피 추출물이 신경세포 사멸에 미치는 영향
Effect of Immature Citrus sunki Peel Extract on Neuronal Cell Death
2
조회
0
다운로드
7
인용
0
참고문헌
키워드
초록
The peel of Citrus sunki exhibits multiple biological activities such as anti-oxidant, anti-inflammation and anti-obesity, but little is known about neurodegeneration-related activities. In this study, we investigated the protective effect of ethanolic extract from both immature and mature Citrus sunki peel on neuronal cell death. Treatment of the neuroblastoma cell line SH-SY5Y with MPP+, an inducer of Parkinson disease model, increased cell death in a dose dependent manner. Increased levels of active caspase-3 and cleaved PARP were detected. Treatment with immature Citrus sunki peel extract significantly reduced MPP+-induced neurotoxicity. Cytoprotection with immature Citrus sunki peel extract was associated with a decrease in caspase-3 activation and PARP cleavage. In contrast, mature Citrus sunki peel extract had no significant effects. These data suggest that immature Citrus sunki peel extract may exert anti-apoptotic effect through the inhibition of caspase-3 signaling pathway on MPP+-induced neuronal cell death.
Abstract
The peel of Citrus sunki exhibits multiple biological activities such as anti-oxidant, anti-inflammation and anti-obesity, but little is known about neurodegeneration-related activities. In this study, we investigated the protective effect of ethanolic extract from both immature and mature Citrus sunki peel on neuronal cell death. Treatment of the neuroblastoma cell line SH-SY5Y with MPP+, an inducer of Parkinson disease model, increased cell death in a dose dependent manner. Increased levels of active caspase-3 and cleaved PARP were detected. Treatment with immature Citrus sunki peel extract significantly reduced MPP+-induced neurotoxicity. Cytoprotection with immature Citrus sunki peel extract was associated with a decrease in caspase-3 activation and PARP cleavage. In contrast, mature Citrus sunki peel extract had no significant effects. These data suggest that immature Citrus sunki peel extract may exert anti-apoptotic effect through the inhibition of caspase-3 signaling pathway on MPP+-induced neuronal cell death.
참고문헌 (26)
- 배광록, "MPP+로 유도된 SH-SY5Y신경세포 사멸에 대한 고분자성분제거 봉독약침액의 신경보호 효과 연구", 대한한방내과학회지, 31(2), pp.254~263 (2010), 대한한방내과학회
- Bredesen DE, "Cell death in the nervous system", Nature, 443, pp.796~802 (2006)
- Choi SY, "Correlation between flavonoid content and the NO production inhibitory activity of peel extracts from various citrus fruits", Biological and Pharmaceutical Bulletin, 30, pp.772~778 (2007)
- Chung SK, "Status of citrus fruit production and view of utilization in Cheju", Food Industry Nutrition, 5, pp.42~52 (2000)
- Delivoria-Papadopoulos M, "ATP and cytochrome c-dependent activation of caspase-9 during hypoxia in the cerebral cortex of newborn piglets", Neruoscience Letters, 429, pp.115~119 (2007)
- Gelb DJ, "Diagnostic criteria for Parkinsin disease", Archives Neurology, 56, pp.33~39 (1999)
- 현재석, "진귤 및 온주밀감 진피의 항산화 활성", 한국식품영양과학회지, 39(1), pp.1~7 (2010), 한국식품영양과학회
- 정영석, "1-methyl-4-phenylpyridinium(MPP+)로 유도된 파킨슨병의 세포 손상에 대한 황백의 신경세포 보호효과", 대한한방내과학회지, 30(1), pp.51~63 (2009), 대한한방내과학회
- 강신해, "제주자생 진귤(Citrus sunki Hort. Tanaka) 과피의 생리활성", 한국식품과학회지, 37(6), pp.923~988 (2005), 한국식품과학회
- Kang SI, "Immature Citrus sunki peel extract exhibits antiobesity effects by beta oxidation and lipolysis in high fat diet induced obese mice", Biological and Pharmaceutical Bulletin, 35, pp.223~230 (2012)
- 김용덕, "수확시기별 제주재래종 감귤과피의 활성산소종 소거활성", 한국식품영양과학회지, 38(4), pp.462~469 (2009), 한국식품영양과학회
- 김영옥, "Neuroprotective Effects of the Extracts from the Aerial Parts of Carthamus tinctorius L. on Transient Cerebral Global Ischemia in Rats", 한국약용작물학회지, 22(1), pp.46~52 (2014), 한국약용작물학회[DOI]
- 이학주, "산소-포도당 결핍(OGD) 유도성 신경세포 사멸에 대한 뇌 보호 효과를 가지는 수종 생약추출물의 검색", 한국약용작물학회지, 17(5), pp.341~345 (2009), 한국약용작물학회
- McGeer PL, "Glial reactions in Parkinson's disease", Movement Disorder, 23, pp.474~483 (2008)
- Nogata Y, "Flavonoid composition of fruit tissues of citrus species", Bioscience, biotechnology, and Biochemistry, 70, pp.178~192 (2006)
- Rock RB, "Microglia as a pharmacological target in infectious and inflammatory diseases of the brain", Journal of Neuroimmune Pharmacology, 1, pp.117~126 (2006)
- 류아름, "Suppressive Effect of Acanthopanax sessiliflorus Extract on the DNA and Cell Damage by Dieldrin", 한국약용작물학회지, 20(4), pp.245~250 (2012), 한국약용작물학회[DOI]
- Hye-Sun Shin, "Anti-inflammatory Effect of the Immature Peel Extract of Jinkyool (Citrus sunki Hort. ex Tanaka)", Food Science and Biotechnology, 20(5), pp.1235~1241 (2011), 한국식품과학회
- Tseng YT, "The chinese herbal formula liuwei dihuang protects dopaminergic neurons against parkinson's toxin through enhancing antioxidative defense and preventing apoptotic death", Phytomedicine, 21, pp.724~733 (2014)
- Wilms H, "Inflammation in Parkinson's diseases and other neurodegenerative diseases : cause and therapeutic implications", Current Pharmceutical Design, 13, pp.1925~1928 (2007)
- Yabuki Y, "Nobiletin treatment improves motor and cognitive deficites seen in MPTP-induced Parkinson model mice", Neuroscience, 259, pp.126~141 (2014)
- Yacoubian TA, "Targets for neuroprotection in Parkinson disease", Biochimica et Biophysica Acta, 1792, pp.676~687 (2008)
- Ye Q, "epigallocatechin-3-gallate suppresses 1-methyl-4-phenylpyridine induced oxidative stress inPC12cells via the SIRT1", PGC-1 signaling pathway, 2012, pp.12, 82~89
- Yi F, "Lycopene protects against MPP+ induced cytotoxicity by maintaining mitochondrial function in SH-SY5Y cells", Neurochemistry Research, 38, pp.1747~1757 (2013)
- Yoshigai E, "Citrus nobiletin suppresses inducible nitric oxide synthase gene expression in interleukin-1β-treated hepatocytes", Biochemistry Biophysical Research Community, 439, pp.54~59 (2013)
- Yusof S, "Naringin content in local citrus fruits", Food Chemistry, 37, pp.113~121 (1990)