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健肺湯이 호흡기 뮤신의 생성 및 유전자 발현에 미치는 영향

Effect of Geonpye-tang(GPT) on Production and Gene Expression of Respiratory Mucin

김호|서운교|정병진
대한한방내과학회 (The Society Of Korean Oriental Internal Medicine)|2009.12|대한한방내과학회지 (The Journal of Korean Oriental Internal Medicine)|제30권 제4호|pp. 685-695

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참고문헌

키워드

Airway mucinGeonpye-tangRTSENCI-H292
주제분류한의학

초록

Objectives : In this study, the author tried to investigate whether Geonpye-tang(GPT) significantly affect PMA- or EGF- or TNF-alpha-induced MUC5AC mucin production and gene expression from human airway epithelial cells. Materials and Methods : Effect of the agent on PMA- or EGF- or TNF-alpha-induced MUC5AC mucin production and gene expression from human airway epithelial cells (NCI-H292) were investigated. Confluent NCI-H292 cells were pretreated for 30 min in the presence of GPT and treated with PMA (10ng/ml) or EGF (25ng/ml) or TNF-alpha (0.2nM), to assess both effect of the agent on PMA- or EGF- or TNF-alpha-induced MUC5AC mucin production by enzyme-linked immunosorbent assay (ELISA) and gene expression by reverse transcription-polymerase chain reaction (RT-PCR). Possible cytotoxicity of the agent was assessed by examining the rate of survival and proliferation of NCI-H292 cells after treatment of agent during 72 hrs (SRB assay). Results : (1) GPT significantly inhibit PMA-induced and EGF-induced MUC5AC mucin productions from NCI-H292 cells. However, GPT did not affect TNF-alpha-induced MUC5AC mucin production ; (2) GPT significantly inhibit the expression levels of PMA- or EGF- or TNF-alpha-induced MUC5AC gene in NCI-H292 cells ; (3) GPT did not show significant cytotoxicity to NCI-H292 cells. Conclusion : This result suggests that GPT can affect the production and gene expression of respiratory mucin observed in diverse respiratory diseases accompanied by mucus hypersecretion. This can explain the traditional use of GPT in oriental medicine. Effect of GPT with their components should be further investigated using animal experimental models that reflect pathophysiology of airway diseases through future studies.

Abstract

Objectives : In this study, the author tried to investigate whether Geonpye-tang(GPT) significantly affect PMA- or EGF- or TNF-alpha-induced MUC5AC mucin production and gene expression from human airway epithelial cells. Materials and Methods : Effect of the agent on PMA- or EGF- or TNF-alpha-induced MUC5AC mucin production and gene expression from human airway epithelial cells (NCI-H292) were investigated. Confluent NCI-H292 cells were pretreated for 30 min in the presence of GPT and treated with PMA (10ng/ml) or EGF (25ng/ml) or TNF-alpha (0.2nM), to assess both effect of the agent on PMA- or EGF- or TNF-alpha-induced MUC5AC mucin production by enzyme-linked immunosorbent assay (ELISA) and gene expression by reverse transcription-polymerase chain reaction (RT-PCR). Possible cytotoxicity of the agent was assessed by examining the rate of survival and proliferation of NCI-H292 cells after treatment of agent during 72 hrs (SRB assay). Results : (1) GPT significantly inhibit PMA-induced and EGF-induced MUC5AC mucin productions from NCI-H292 cells. However, GPT did not affect TNF-alpha-induced MUC5AC mucin production ; (2) GPT significantly inhibit the expression levels of PMA- or EGF- or TNF-alpha-induced MUC5AC gene in NCI-H292 cells ; (3) GPT did not show significant cytotoxicity to NCI-H292 cells. Conclusion : This result suggests that GPT can affect the production and gene expression of respiratory mucin observed in diverse respiratory diseases accompanied by mucus hypersecretion. This can explain the traditional use of GPT in oriental medicine. Effect of GPT with their components should be further investigated using animal experimental models that reflect pathophysiology of airway diseases through future studies.

참고문헌 (34)

  1. Newhouse, M.T, "Respiratory tract defense mechanism, In, textbook of pulmonary disease 3rd ed" (1983), Little Brown and Company
  2. Frigas, E, "Elevated levels of the eosinophil granule major basic protein in the sputum of patients with bronchial asthma. Mayo Clin", Proc, 56, pp.345~353 (1981)
  3. Culpitt SV, "Sputum matrix metalloproteases: comparison between chronic obstructive pulmonary disease and asthma", Respir Med, 99(6), pp.703~710 (2005)
  4. Gleich, G.J, "The eosinophil and bronchial asthma: Current understanding", J. Allergy Clin. Immunol, 85, pp.422~436 (1990)
  5. Rogers DF, "Airway mucus hypersecretion in asthma: an undervalued pathology?", Curr Opin Pharmacol, 4(3), pp.241~250 (2004)
  6. Mutschler, E, "Drug actions", CRC press, Inc, pp.410~411 (1995)
  7. Rogers DF, "Treatment of airway mucus hypersecretion", Ann Med, 38(2), pp.116~125 (2006)
  8. Rogers DF, "Mucociliary dysfunction in COPD: effect of current pharmacotherapeutic options", Pulm Pharmacol Ther, 18(1), pp.1~8 (2005)
  9. 한의과대학 방제학교수, "방제학", pp.98~ (2003), 영림사
  10. 이승언, "蔘蘇飮의 항알레르기 效果", 대한한방내과학회지, 26(1), pp.1~15 (2005), 대한한방내과학회
  11. 류내형, "삼소음 알코올 추출물이 생쥐 면역세포의 시토킨 조절에 미치는 효과", 동의생리병리학회지, 22(4), pp.856~862 (2008), 대한동의병리학회
  12. Nam BS, "The experimental study on the anti-allergic effects of Samsoeum and Samsoeumgamibang", The Journal of Oriental Medical Surgery, Ophthalmology & Otolaryngology, 15(1), pp.343~355 (2002)
  13. Choi SB, "A study of effects of Smasoeum on the analgesia, Antipyresis and O3 posisoned lung in rats and mice", K. H. Univ. O. Med. J, 8, pp.139~148 (1985)
  14. 류인선, "加味治哮散 및 加味理中湯이 氣道 喀痰 분비에 미치는 영향", 동의생리병리학회지, 18(6), pp.1746~1750 (2004), 대한동의병리학회
  15. 박양춘, "加味六君子湯이 호흡기 뮤신분비 및 뮤신 유전자 발현에 미치는 영향", 동의생리병리학회지, 21(1), pp.98~103 (2007), 대한동의병리학회
  16. 한달수, "加味腎氣湯 및 加味淸肺湯이 기도점액 분비 및 기관평활근 긴장도에 미치는 영향", 동의생리병리학회지, 20(1), pp.156~162 (2006), 대한동의병리학회
  17. 박정준, "紫？治哮散과 瓜蔞枳實湯이 일차배양된 齧齒類 氣道 杯狀細胞에서의 뮤신 분비에 미치는 영향", 동의생리병리학회지, 20(1), pp.69~75 (2006), 대한동의병리학회
  18. 김영호, "加味鎭咳湯이 호흡기 점액의 mucin분비에 미치는 영향", 대한한의학회지, 29(3), pp.63~75 (2008), 대한한의학회
  19. 서운교, "杏蘇湯 및 取淵湯이 호흡기 뮤신 유전자의 발현과 점액분비에 미치는 영향", 대한한의학회지, 29(3), pp.76~87 (2008), 대한한의학회
  20. 박완열, "Mucin 분비에 미치는 大靑龍湯 및 <石室秘？＞急治法方에 대한 연구", 대한한방내과학회지, 27(1), pp.92~101 (2006), 대한한방내과학회
  21. 심성흠, "射干麻黃湯 및 <石室秘錄＞逆醫法方이 호흡기 점액의 분비에 미치는 영향", 대한한방내과학회지, 27(2), pp.295~304 (2006), 대한한방내과학회
  22. 박완열, "淸燥救肺湯과 理陰煎이 호흡기 점액분비에 미치는 영향", 대한한방내과학회지, 29(2), pp.318~333 (2008), 대한한방내과학회
  23. 김호, "小靑龍湯加石膏 및 ≪石室秘？≫小治法方이 호흡기 점액의 분비에 미치는 영향", 대한한방내과학회지, 27(4), pp.895~904 (2006), 대한한방내과학회
  24. Lee, C.J, "Specificity in the inhibition of mucin release from airway goblet cells by polycationic peptides", J. Appl. Pharmacol, 9(3), pp.218~223 (2001)
  25. Ko, K.H, "Inhibition of mucin release from airway goblet cells by polycationic peptides", Am. J. Physiol, 277(21), pp.L811~L815 (1999)
  26. Shao MXG, "Tumor necrosis factor alpha-converting enzyme mediates MUC5AC mucin expression in cultured human airway epithelial cells", Proc Natl Acad Sci USA, 100, pp.11618~11623 (2003)
  27. Song KS, "Interleukin-1β and Tumor Necrosis factor-alpha Induce MUC5AC overexpression through a mechanism involving ERK", p38 mitogen-activated protein kinases- MSK1-CREB activation in human airway epithelial cells, 2003, pp.278, 23243~23250
  28. Karlinsey, J, "Simultaneous purification of DNA and RNA from small numbers of eukaryotic cells", Anal. Biochem, 180(2), pp.303~306 (1989)
  29. Kim YD, "Interleukin-1beta induces MUC2 and MUC5AC synthesis through cyclooxygenase-2 in NCI-H292 cells", Mol. Pharmacol, 62(5), pp.1112~1118 (2002)
  30. Skehan P, "New Colorimetric Cytotoxicity Assay for Anticancer Drug Screening", J. Natl. Cancer Inst, 82, pp.1107~1112 (1990)
  31. 전국한의과대학 공동교수편집위원회, "本草學", pp.289~290 (2000), 永林社
  32. Schmidt, R, "Autoxidation of phorbol esters under normal storage conditions", Cancer Res, 35, pp.1375~1377 (1975)
  33. Saitoh, T, "Protein kinase C in human brain and its inhibition by calmodulin", Brain Res, 379, pp.196~199 (1986)
  34. Li D, "Cloning of the amino-terminal and 5'-flanking region of the human MUC5AC mucin gene and transcriptional up-regulation by bacterial exoproducts", J Biol Chem, 273, pp.6812~6820 (1998)

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