淸燥救肺湯과 理陰煎이 호흡기 점액분비에 미치는 영향
Effects of Cheongjogupye-tang (淸燥救肺湯) and Yieum-jeon (理陰煎) on Secretion of Mucin from Respiratory Epithelial Cells
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Objectives : In this study, the author tried to examine whether Cheogjogupye-tang (淸燥救肺湯, CGPT) and Yieum-jeon (理陰煎, YEJ) significantly affect in vitro and in vivo mucin secretion, MUC5AC gene expression in airway epithelial cells and contractility of isolated tracheal smooth muscle of rabbit. Materials and Methods : For in vitro experiment, confluent hamster tracheal surface epithelial (HTSE) cells were chased for 30 minutes in the presence of CGPT and YEJ to assess the effects of the agents on mucin secretion by enzyme-linked immunosorbent assay (ELISA), with removal of oriental herbal medicine extract from each agent-treated sample by centrifuge microfilter. Also, the effects of the agents on TNF-alpha or EGF-induced MUC5AC gene expression in human airway epithelial cells (NCI-H292) were investigated. Possible cytotoxicities of the agent were assessed by examining both LDH release from HTSE cells and the rate of survival and proliferation of NCI-H292 cells. For in vivo experiment, hypersecretion of airway mucin and goblet cell hyperplasia was induced by exposure of rats to SO2 over 3 weeks. Effects of CGPT and YEJ orally administered for 1 week on in vivo mucin secretion from tracheal goblet cells of rats and hyperplasia of goblet cells were assessed using ELISA and histological analysis after staining the epithelial tissue with alcian blue, respectively. Also, the effects of CGPT and YEJ on contractility of isolated tracheal smooth muscle were investigated. Results : (1) CGPT significantly inhibited in vitro mucin secretion from cultured HTSE cells. However, YEJ did not affect in vitro mucin secretion; (2) CGPT and YEJ did not affect hypersecretion of in vivo mucin and hyperplasia of tracheal goblet cells; (3) CGPT and YEJ slightly increased the expression levels of TNF-alpha or EGF-induced MUC5AC gene in NCI-H292 cells; (4) CGPT and YEJ inhibited acetylcholine-induced contraction of isolated tracheal smooth muscle of rabbit; (5) CGPT and YEJ did not affect LDH release from HTSE cells and the survival and proliferation of NCI-H292 cells. Conclusion : The results from the present study suggest that CGPT and YEJ mainly affect the expression of mucin gene rather than secretion of mucin and do not show remarkable cytotoxicity to respiratory epithelial cells.
Abstract
Objectives : In this study, the author tried to examine whether Cheogjogupye-tang (淸燥救肺湯, CGPT) and Yieum-jeon (理陰煎, YEJ) significantly affect in vitro and in vivo mucin secretion, MUC5AC gene expression in airway epithelial cells and contractility of isolated tracheal smooth muscle of rabbit. Materials and Methods : For in vitro experiment, confluent hamster tracheal surface epithelial (HTSE) cells were chased for 30 minutes in the presence of CGPT and YEJ to assess the effects of the agents on mucin secretion by enzyme-linked immunosorbent assay (ELISA), with removal of oriental herbal medicine extract from each agent-treated sample by centrifuge microfilter. Also, the effects of the agents on TNF-alpha or EGF-induced MUC5AC gene expression in human airway epithelial cells (NCI-H292) were investigated. Possible cytotoxicities of the agent were assessed by examining both LDH release from HTSE cells and the rate of survival and proliferation of NCI-H292 cells. For in vivo experiment, hypersecretion of airway mucin and goblet cell hyperplasia was induced by exposure of rats to SO2 over 3 weeks. Effects of CGPT and YEJ orally administered for 1 week on in vivo mucin secretion from tracheal goblet cells of rats and hyperplasia of goblet cells were assessed using ELISA and histological analysis after staining the epithelial tissue with alcian blue, respectively. Also, the effects of CGPT and YEJ on contractility of isolated tracheal smooth muscle were investigated. Results : (1) CGPT significantly inhibited in vitro mucin secretion from cultured HTSE cells. However, YEJ did not affect in vitro mucin secretion; (2) CGPT and YEJ did not affect hypersecretion of in vivo mucin and hyperplasia of tracheal goblet cells; (3) CGPT and YEJ slightly increased the expression levels of TNF-alpha or EGF-induced MUC5AC gene in NCI-H292 cells; (4) CGPT and YEJ inhibited acetylcholine-induced contraction of isolated tracheal smooth muscle of rabbit; (5) CGPT and YEJ did not affect LDH release from HTSE cells and the survival and proliferation of NCI-H292 cells. Conclusion : The results from the present study suggest that CGPT and YEJ mainly affect the expression of mucin gene rather than secretion of mucin and do not show remarkable cytotoxicity to respiratory epithelial cells.
참고문헌 (36)
- 崔亭柱, "黃帝內經素問", pp.291~ (2004), 자유문고
- 朱丹溪, "丹溪心法", pp.410~411 (1995), 杏林書院
- 喩昌, "醫門法律", pp.55~ (1998), 湖南科學技術出版社
- 張介賓, "景岳全書", pp.2602~2603 (2006), 한미의학
- Newhouse M, "Respiratory tract defense mechanism: In textbook of pulmonary disease", pp.2~47 (1983), Little Brown and Company
- Frigas E, "Elevated levels of the eosinophil granule major basic protein in the sputum of patients with bronchial asthma", Mayo Clin Proc, 56, pp.345~353 (1981)
- Culpitt SV, "Sputum matrix metalloproteases: comparison between chronic obstructive pulmonary disease and asthma", Respir Med, 99(6), pp.703~710 (2005)
- Gleich GJ, "The eosinophil and bronchial asthma: Current understanding", Journal Allergy Clin Immunol, 85, pp.422~436 (1990)
- Rogers DF, "Airway mucus hypersecretion in asthma: an undervalued pathology", Curr Opin Pharmacol, 4(3), pp.241~250 (2004)
- Mutschler E, "Drug actions", pp.410~411 (1995), CRC press
- 趙仁珠, "인체폐암세포에서 Bcl-2 발현저하 및 caspace 활성을 통한 淸燥救肺湯의 apoptosis 유발에 관한 연구" (2006), 동의대학교 대학원
- 林在炯, "淸燥救肺湯이 인체폐암세포의 증식 억제 및 prostaglandin E 생성 억제에 관한 연구" (2006), 동의대학교 대학원
- 박민철, "淸燥救肺湯의 항암 및 항알레르기 효과에 관한 연구" (2003), 우석대학교 대학원
- 柳東昊, "實脾飮 및 理陰煎이 Gentamicin Sulfate로 誘發된 白鼠의 腎損傷에 미치는 影響", 경희의학, 9(2), pp.179~194 (1993)
- Lee CJ, "Specificity in the inhibition of mucin release from airway goblet cells by polycationic peptides", J Appl Pharmacol, 9(3), pp.218~223 (2001)
- Kim KC, "Biochemical characterization of mucous glycoproteins synthesized and secreted by hamster tracheal epithelial cells in primary culture", J Biol Chem, 260, pp.4021~4027 (1985)
- Kim KC, "Possible requirement of collagen gel substratum for production of mucinlike glycoproteins by primary rabbit tracheal epithelial cells in culture", In Vitro, 21, pp.617~621 (1985)
- Kim KC, "Gel contraction causes mucin release in primary hamster tracheal epithelial cells growing on a collagen gel", J Cell Biol, 105, pp.158~ (1987)
- Wu R, "Continuous multiplication of rabbit tracheal epithelial cells in a defined, homone-supplemented medium", In Vitro, 18, pp.800~812 (1982)
- Wu R, "Expression of tracheal differentiated function in serum-free hormone-supplemented medium", J Cell Physiol, 125, pp.167~181 (1985)
- Ko KH, "Inhibition of mucin release from airway goblet cells by polycationic peptides", Am J Physiol, 277(21), pp.811~815 (1999)
- Shao MXG, "Tumor necrosis factor alpha-converting enzyme mediates MUC5AC mucin expression in cultured human airway epithelial cells", Proc. Natl Acad Sci USA, 100, pp.11618~11623 (2003)
- Song KS, "Interleukin-1β and Tumor Necrosis factor-alpha Induce MUC5AC overexpression through a mechanism involving ERK", p38 mitogen-activated protein kinases- MSK1-CREB activation in human airway epithelial cells, 2003, pp.278, 23243~24250
- Harkema JR, "In vivo effects of endotoxin on intraepithelial mucosubstances in rat pulmonary airways: quantitative histochemistry", Am J Pathol, 141, pp.307~331 (1992)
- St George JA, "An immunohistochemical characterization of Rhesus monkey respiratoty secretions using monoclonal antibodies", Am Rev Resp Dis, 132, pp.556~563 (1985)
- Karlinsey J, "Simultaneous purification of DNA and RNA from small numbers of eukaryotic cells", Anal Biochem, 180(2), pp.303~306 (1989)
- Skehan P, "New Colorimetric Cytotoxicity Assay for Anticancer Drug Screening", J Nat. Cancer Inst, 82, pp.1107~1112 (1990)
- 全國韓醫科大學 肺係內科學敎室, "肺系內科學", pp.103~ (2004), 도서출판 국진
- 李中梓, "醫宗必讀", pp.540~ (1994), 人民衛生出版社
- 黃泰康, "中醫哮病學", pp.5~7 (2002), 中國醫藥科技出版社
- 金相贊, "方劑學", pp.232~234 (1999), 圖書出版 永林社
- 黃度淵, "方藥合編", pp.348~ (2000), 南山堂
- Kim KC, "Secretions from primary hamster tracheal surface epithelial cells in culture: Mucin-like glycoproteins, proteoglycans, and lipids", Exp Lung Res, 15, pp.299~314 (1989)
- Rogers DF, "Treatment of airway mucus hypersecretion", Ann Med, 38(2), pp.116~125 (2006)
- Rogers DF, "Mucociliary dysfunction in COPD: effect of current pharmacotherapeutic options", Pulm Pharmacol Ther, 18(1), pp.1~8 (2005)
- Yu XY, "Physiologic modulation of bronchial epithelial cell barrier function by polycationic exposure", Am J Respir Cell Mol. Biol, 11, pp.188~198 (1994)