循環器加減方이 고지혈증 유발 白鼠에 미치는 영향
Effect of Sunhwangigagambang(SHG) on Hyperlipidemia in SD Rats Induced by High Cholesterol Diet
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Objective : This study was performed to investigate the effect of Sunhwangigagambang(SHG) on hyperlipidemia in SD rats induced by high cholesterol diet. Method : After treatment with SHG, cytotoxicity, body weight, liver weight, AST, ALT, ALP, total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, glucose, albumin, total protein in serum, malondialdehyde, and gene expression for ACAT and HMG-CoA reductase in hepatic tissue were analyzed. Result :1. SHG didn't show any cytotoxicity in both human fibroblast cell line and SD rats. 2. SHG significantly inhibited the increase of liver weight by high cholesterol diet compared to the control group. 3. SHG significantly ameliorated the increase of total cholesterol, LDL-cholesterol, and triglyceride and reduction of HDL-cholesterol compared to the control group. 4. SHG significantly reduced glucose level in serum compared to the control group. 5. SHG significantly reduced malondialdehyde in hepatic tissue compared to the control group. 6. SHG significantly down-regulated gene expression of ACAT and HMG-CoA reductase compared to the control group. Conclusion : These results suggest that SHG might be effective in treatment and prevention of hyperlipidemia.
Abstract
Objective : This study was performed to investigate the effect of Sunhwangigagambang(SHG) on hyperlipidemia in SD rats induced by high cholesterol diet. Method : After treatment with SHG, cytotoxicity, body weight, liver weight, AST, ALT, ALP, total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, glucose, albumin, total protein in serum, malondialdehyde, and gene expression for ACAT and HMG-CoA reductase in hepatic tissue were analyzed. Result :1. SHG didn't show any cytotoxicity in both human fibroblast cell line and SD rats. 2. SHG significantly inhibited the increase of liver weight by high cholesterol diet compared to the control group. 3. SHG significantly ameliorated the increase of total cholesterol, LDL-cholesterol, and triglyceride and reduction of HDL-cholesterol compared to the control group. 4. SHG significantly reduced glucose level in serum compared to the control group. 5. SHG significantly reduced malondialdehyde in hepatic tissue compared to the control group. 6. SHG significantly down-regulated gene expression of ACAT and HMG-CoA reductase compared to the control group. Conclusion : These results suggest that SHG might be effective in treatment and prevention of hyperlipidemia.
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