Effects of Electroacupuncture on Immobilization Stress Responses : A Study on Inhibitory Avoidance Task, Forced Swimming Test, and Stress Hormones
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Objectives : This study investigated the effects of electroacupuncture on memory, depression, and plasma stress hormone levels in rats that were under immobilization stress. Materials and Methods : The immobilization-only group was given two hours of immobilization stress for 10 consecutive days. The immobilization and high frequency EA group was given two hours of immobilization stress simultaneously with high frequency (100Hz) electroacupuncture stimulation on the right ST-36 (Zusanli) for 10 consecutive days. We conducted the inhibitory avoidance and forced swimming tests to recognize whether immobilization stress and electroacupuncture have effects on memory and depression. We collected blood samples from the tail of each rat at 30, 60, 90, and 120-minute intervals during the immobilization stress and EA stimulation to measure plasma concentrations of ACTH, corticosterone, melatonin, and norepinephrine induced by immobilization stress and electroacupuncture. Results : There was a significant effect of high frequency on the increase in anamnesis based on the result of the inhibitory avoidance test, but there was no significant effect of decreasing depression based on the result of the forced swim test. Also, there was no significant effect on the response indicated by stress hormones. Conclusions : 1. High frequency electroacupuncture (100Hz) improved anamnesis in immobilization stress states under the inhibitory avoidance task. 2. High frequency electroacupuncture (100Hz) did not reduce depression induced by immobilization stress under the forced swimming test. 3. High frequency electroacupuncture (100Hz) did not decrease stress hormones through blood sampling.
Abstract
Objectives : This study investigated the effects of electroacupuncture on memory, depression, and plasma stress hormone levels in rats that were under immobilization stress. Materials and Methods : The immobilization-only group was given two hours of immobilization stress for 10 consecutive days. The immobilization and high frequency EA group was given two hours of immobilization stress simultaneously with high frequency (100Hz) electroacupuncture stimulation on the right ST-36 (Zusanli) for 10 consecutive days. We conducted the inhibitory avoidance and forced swimming tests to recognize whether immobilization stress and electroacupuncture have effects on memory and depression. We collected blood samples from the tail of each rat at 30, 60, 90, and 120-minute intervals during the immobilization stress and EA stimulation to measure plasma concentrations of ACTH, corticosterone, melatonin, and norepinephrine induced by immobilization stress and electroacupuncture. Results : There was a significant effect of high frequency on the increase in anamnesis based on the result of the inhibitory avoidance test, but there was no significant effect of decreasing depression based on the result of the forced swim test. Also, there was no significant effect on the response indicated by stress hormones. Conclusions : 1. High frequency electroacupuncture (100Hz) improved anamnesis in immobilization stress states under the inhibitory avoidance task. 2. High frequency electroacupuncture (100Hz) did not reduce depression induced by immobilization stress under the forced swimming test. 3. High frequency electroacupuncture (100Hz) did not decrease stress hormones through blood sampling.
참고문헌 (28)
- 조장희, "침의 치료기전에 대한 신경기반 및 신경기능 가설 -침자극과 관계된 신경기반 및 체액성 반응, 신경적 반응, 면역반응", 대한침구의학회지, 20(5), pp.14~186 (2003), 대한침구의학회
- Imai K, "Electroacupuncture improves imbalance of autonomic function under restraint stress in conscious rats", Am J Chin Med, 37(1), pp.45~55 (2009)
- Alexis DS, "Chronic stress decreases the expression of sympathetic markers in the pineal gland and increases plasma melatonin concentration in rats", Journal of Neurochemistry, 97, pp.1279~1287 (2006)
- Yang CH, "Effect of electroacupuncture on response to immobilization", Pharmacology, Biochemistry and Behavior, 72, pp.847~855 (2002)
- 강세영, "전기침이 면역증진에 미치는 효과", 대한침구의학회지, 24(6), pp.29~36 (2007), 대한침구의학회
- Sun H, "Effect of acupuncture at Baihui (GV 20) and Zusanli (ST 36) on the level of serum inflammatory cytokines in patients with depression", Zhongguo Zhen Jiu, 30(3), pp.195~199 (2010)
- Guimaraes C.M, "Effects of acupuncture on behavioral, cardiovascular and hormonal responses in restraint -stressed Wistar rats", J Med Biol Res, 30(12), pp.1445~1450 (1997)
- 정은우, "Effects of Electroacupuncture on Plasma Stress Hormone Responses to Acute and Chronic Immobilization Stress", 대한침구의학회지, 27(5), pp.105~115 (2010), 대한침구의학회
- Eric RA, "The inhibitory aboidance test optimized for discovery of cognitive enhancers", Behavior Research Methods, 41(3), pp.805~811 (2009)
- Craft RM, "Forced swim test behavior in postpartum rats", Pharmacology, Biochemistry and Behavior, 96, pp.402~412 (2010)
- Alexies DS, "Chronic stress decreases the expression of sympathetic markers in the pineal gland and increases plasma melatonin concentration in rats", Journal of Neurochemistry, 97(5), pp.1279~1287 (2006)
- Magarinos AM, "Stress-induced atrophy of apical dendrites of hippocampal CA3c neurons: involvement of glucocorticoid secretion and excitatory amino acid receptors", Neuroscience, 69(1), pp.89~98 (1995)
- Sheline YI, "Hippocampal atrophy in recurrent major depression", Proc Natl Acad Sci USA, 93, pp.3908~3913 (1996)
- Sapolsky RM, "Depression, antidepressants, and the shrinking hippocampus", Proc Natl Acad Sci USA, 98(22), pp.12320~12322 (2001)
- Duan DM, "Efficacy evaluation for depression with somatic symptoms treated by electroacupuncture combined with Fluoxetine", J Tradit Chin Med, 29(3), pp.167~173 (2009)
- Chrousos G, "The concepts of stress and stress system disorders", JAMA, 267, pp.1244~1252 (1992)
- McEwen B, "Protective and damaging effects of stress mediators", New England Journal of Medicine, 338, pp.171~179 (1998)
- Melamed S, "Chronic burnout, somatic arousal, and elevated salivary cortisol levels", J Psychosom Res, 46, pp.591~598 (1999)
- Steptoe A, "Job strain and anger expression predict early morning elevations in salivary cortisol", Psychosom Med, 62, pp.286~292 (2000)
- Vedhara K, "Chronic stress in elderly carers of dementia patients and antibody response to influenza vaccination", The Lancet, l353, pp.627~631 (1999)
- Cao L, "Sympathetic nervous system plays a major role in acute cold", restraint stress inhibition of host resistance to Listeria monocytogenes, 2002, pp.125, 94~102
- Mason JW, "A review of psychoendocrine research on the sympathetic-adrenal medullary system", Psychosom Med, 30, pp.631~653 (1968)
- Lupien SJ, "acute effects of corticosteroids on cognition: integration of animal and human model studies", Brain Res Rev, 24, pp.1~27 (1997)
- Vera Klenerova, "Combined restraint and cold stress in rats: effects on memory processing in passive avoidane task and on plasma levels of ACTH and corticosterone", Behavioural Brain Research, 142, pp.143~149 (2003)
- Borrell J, "Inhibitory avoidance deficit following short- term adrenalectomy in the rat: the role of adrenal catecholamines", Behav Neural Biol, 39, pp.241~258 (1983)
- Laudon M, "Ontogeny of prolactin releasing and inhibiting activities in the posterior pituitary of male rats", Neuroendocrinology, 50, pp.644~649 (1989)
- Lynch HJ, "Control of pineal indole biosynthesis by changes in sympathetic tone caused by factors other than environmental lighting", Natl Acad Sci USA, 70, pp.1704~1707 (1973)
- Vogel WH, "Chronic stress and plasma catecholamine and corticosterone levels in male rats", Neurosci Lett, 87, pp.183~188 (1988)