Scolopendra Pharmacopuncture Ameliorates Behavioral Despair in Mice Stressed by Chronic Restraint
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Introduction: Pharmacopuncture, which combines acupuncture with herbal medicine, is one of the newly developed acupuncture techniques that has recently been put into use. The possible mechanisms of scolopendra pharmacopuncture, as well as its potential effects on depressive symptoms, were investigated in this study by using a mouse model of chronic immobilization stress (CIS). Methods: C57BL/6 male mice were randomly assigned into three groups: mice not stressed with restraint and injected with distilled water, mice stressed with restraint and injected with distilled water, and mice stressed with restraint injected with scolopendra pharmacopuncture at a cervical site. Behavioral tests (an open field test, tail suspension test, and forced swimming test) were carried out after two weeks of CIS and injection treatments. The expression levels of glial fibrillary acidic protein (GFAP) in the hippocampus were determined by using western blot and immunohistochemistry analyses. Results: Mice exposed to CIS showed decreased behavioral activity, while scolopendra pharmacopuncture treatment significantly protected against the depressive-like behaviors induced by CIS. Moreover, scolopendra pharmacopuncture treatment increased GFAP protein levels in the hippocampi of the mice stressed by chronic immobilization. Conclusion: Scolopendra pharmacopuncture has an ameliorating effect on depressive behavior, which is partially mediated through protection against glial loss in the hippocampus.
Abstract
Introduction: Pharmacopuncture, which combines acupuncture with herbal medicine, is one of the newly developed acupuncture techniques that has recently been put into use. The possible mechanisms of scolopendra pharmacopuncture, as well as its potential effects on depressive symptoms, were investigated in this study by using a mouse model of chronic immobilization stress (CIS). Methods: C57BL/6 male mice were randomly assigned into three groups: mice not stressed with restraint and injected with distilled water, mice stressed with restraint and injected with distilled water, and mice stressed with restraint injected with scolopendra pharmacopuncture at a cervical site. Behavioral tests (an open field test, tail suspension test, and forced swimming test) were carried out after two weeks of CIS and injection treatments. The expression levels of glial fibrillary acidic protein (GFAP) in the hippocampus were determined by using western blot and immunohistochemistry analyses. Results: Mice exposed to CIS showed decreased behavioral activity, while scolopendra pharmacopuncture treatment significantly protected against the depressive-like behaviors induced by CIS. Moreover, scolopendra pharmacopuncture treatment increased GFAP protein levels in the hippocampi of the mice stressed by chronic immobilization. Conclusion: Scolopendra pharmacopuncture has an ameliorating effect on depressive behavior, which is partially mediated through protection against glial loss in the hippocampus.
참고문헌 (37)
- Kessler RC, "The epidemiology of major depressive disorder : results from the National Comorbidity Survey Replication(NCS-R)", JAMA, 289(23), pp.3095~3105 (2003)
- Fava M, "Major Depressive Disorder", Neuron, 28(2), pp.335~341 (2000)
- Leo RJ, "A systematic review of randomized controlled trials of acupuncture in the treatment of depression", J Affect Disord, 97(1), pp.13~22 (2007)
- Smith CA, "Acupuncture for depression", Cochrane Database Syst Rev, (1), pp.Cd004046~ (2010)
- Sarris J, "Herbal medicine for depression, anxiety and insomnia : a review of psychopharmacology and clinical evidence", Eur Neuropsychopharmacol, 21(12), pp.841~860 (2011)
- Ng QX, "Clinical use of Hypericum perforatum(St John's wort)in depression : A meta-analysis", J Affect Disord, 210, pp.211~221 (2017)
- Litscher G, "High-tech laser acupuncture is Chinese medicine", Med Acupunct, 20(4), pp.245~254 (2008)
- Daniela Litscher, "Korean Pharmacopuncture Meets Austrian High- Tech Acupuncture - A Short Review Article Including a Bibliometric Analysis of Pharmacopuncture over the Last 15 Years - Pharmacopuncture and Europe", Journal of Pharmacopuncture, 16(1), pp.7~11 (2013), 대한약침학회[DOI]
- Lee YJ, "Usage report of pharmacopuncture in musculoskeletal patients visiting Korean medicine hospitals and clinics in Korea", BMC complementary and alternative medicine, 16(1), pp.292~ (2016)
- 최민지, "Effects of Sumsu (Bufonis venenum) Pharmacopuncture Treatment on Depression in Mice", Journal of Pharmacopuncture, 17(2), pp.27~33 (2014), 대한약침학회[DOI]
- 서동균, "蟾酥약침이 수면장애 환자의 불면 및 우울 증상에 미치는 영향에 대한 임상적 고찰", Journal of Acupuncture Research, 32(3), pp.175~183 (2015), 대한침구의학회
- 김성철, "蜈蚣藥鍼에 대한 硏究現況分析과 治療法에 관한 硏究", Journal of Pharmacopuncture, 9(2), pp.113~127 (2006), 대한약침학회
- Fishbain D, "Evidence-based data on pain relief with antidepressants", Ann Med, 32(5), pp.305~316 (2000)
- 조계윤, "Stability Test and Quantitative and Qualitative Analyses of the Amino Acids in Pharmacopuncture Extracted from Scolopendra subspinipes mutilans", Journal of Pharmacopuncture, 18(1), pp.44~55 (2015), 대한약침학회[DOI]
- Vyas A, "Chronic stress induces contrasting patterns of dendritic remodeling in hippocampal and amygdaloid neurons", J Neurosci, 22(15), pp.6810~6818 (2002)
- Roozendaal B, "Stress, memory and the amygdala", Nat Rev Neurosci, 10(6), pp.423~ (2009)
- Wanasuntronwong A, "Anxiolytic effects of standardized extract of Centella asiatica(ECa 233)after chronic immobilization stress in mice", J Ethnopharmacol, 143(2), pp.579~585 (2012)
- Kim JH, "Electroacupuncture acutely improves cerebral blood flow and attenuates moderate ischemic injury via an endothelial mechanism in mice", PLoS One, 8(2), pp.e56736~ (2013)
- Seibenhener ML, "Use of the Open Field Maze to measure locomotor and anxiety-like behavior in mice", J Vis Exp, (96) (2015)
- Steru L, "The tail suspension test : a new method for screening antidepressants in mice", Psychopharmacology, 85(3), pp.367~370 (1985)
- Can A, "The mouse forced swim test", J Vis Exp, (59) (2012)
- Rial D, "Depression as a glial-based synaptic dysfunction", Front Cell Neurosci, 9 (2015)
- Schroeter ML, "Mood disorders are glial disorders:evidence from in vivo studies", Cardiovasc Psychiatry Neurol, 2010 (2010)
- Cobb JA, "Density of GFAP-immunoreactive astrocytes is decreased in left hippocampi in major depressive disorder", Neuroscience, 316, pp.209~220 (2016)
- Li L-F, "Magnolol treatment reversed the glial pathology in an unpredictable chronic mild stress-induced rat model of depression", Eur J Pharmacol, 711(1), pp.42~49 (2013)
- Liu Q, "Glia atrophy in the hippocampus of chronic unpredictable stress-induced depression model rats is reversed by electroacupuncture treatment", J Affect Disord, 128(3), pp.309~313 (2011)
- Liu Q, "Clomipramine treatment reversed the glial pathology in a chronic unpredictable stress-induced rat model of depression", Eur Neuropsychopharmacol, 19(11), pp.796~805 (2009)
- 이광호, "History of Research on Pharmacopuncture in Korea", Journal of Pharmacopuncture, 19(2), pp.101~108 (2016), 대한약침학회[DOI]
- 이순이, "産後 憂鬱症을 紫河車 藥鍼을 활용하여 治療한 1例", 대한한방부인과학회지, 19(2), pp.282~294 (2006), 대한한방부인과학회
- 제준태, "뇌졸중 후 우울증에 대한 황련해독탕 약침의 안전성 및 유효성 평가를 위한 임상 시험", Journal of Pharmacopuncture, 13(2), pp.67~74 (2010), 대한약침학회
- 김선욱, "三陰交(SP6)ㆍ足三里(ST36) 白茯笭藥鍼이 만성 스트레스 모델 흰쥐에 미치는 영향", Journal of Acupuncture Research, 32(2), pp.187~195 (2015), 대한침구의학회
- Xiaohong L, "Effect of moxibustion or acupuncture in Dazhui(GV14)on behavior and hypothalamic AVP level in rats with chronic stress disturbance", Journal of beijing university of traditional chinese medicine, 28(6), pp.47~ (2005)
- Xu S, "Effects of electroacupuncture on depression in a rat model", Acupunct Electrother Res, 36(3-4), pp.259~273 (2011)
- Jiang J, "Anti-depression effect of acupuncture and moxibustion based on SSRIs medication", Zhongguo Zhen Jiu, 32(3), pp.219~223 (2012)
- Sun P, "The effect of the acupuncture intervention of dredging Governor Vessel and regulating mentality for the medication treatment of post-stroke depression", Zhongguo Zhen Jiu, 35(8), pp.753~757 (2015)
- Gomes A, "Isolation, purification & pharmacodynamics of a toxin from the venom of the centipede Scolopendra subspinipes dehaani Brandt", Indian J Exp Biol, 21(4), pp.203~207 (1983)
- 이정진, "Inhibitory Effects of Scolopendra Pharmacopuncture on the Development and Maintenance of Neuropathic Pain in Rats: Possible Involvement of Spinal Glial Cells", Journal of Acupuncture & Meridian Studies, 8(5), pp.236~244 (2015), 사단법인약침학회[DOI]