아무르 불가사리(Asterias amurensis)의 관족으로부터 신경성 펩타이드의 분리 및 정제
Isolation and Purification of Neuropeptides from the Tube Feet of the Starfish Asterias amurensis
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Two neuropeptides were purified from the acidified tube feet extract of the starfish Asterias amurensis by C18 re- versed phase and size-exclusion high-performance liquid chromatography (HPLC). The tube feet extract and the pu- rified peptides (AST-I and AST-II) showed potent contractile activity on dorsal retractor muscle (DRM) of the starfish Asterina pectinifera and intestine (smooth muscle) of the panther puffer Takifugu pardalis . Treatment of the purified peptides with dithiothreitol (DTT) for 60 min at 37°C significantly altered their retention times, suggesting that these compounds contained disulfide bonds. The molecular weights of AST-I and AST-II were determined to be 2064.2 Da and 6137.2 Da, respectively, by MALDI-TOF mass spectrometry.
Abstract
Two neuropeptides were purified from the acidified tube feet extract of the starfish Asterias amurensis by C18 re- versed phase and size-exclusion high-performance liquid chromatography (HPLC). The tube feet extract and the pu- rified peptides (AST-I and AST-II) showed potent contractile activity on dorsal retractor muscle (DRM) of the starfish Asterina pectinifera and intestine (smooth muscle) of the panther puffer Takifugu pardalis . Treatment of the purified peptides with dithiothreitol (DTT) for 60 min at 37°C significantly altered their retention times, suggesting that these compounds contained disulfide bonds. The molecular weights of AST-I and AST-II were determined to be 2064.2 Da and 6137.2 Da, respectively, by MALDI-TOF mass spectrometry.
참고문헌 (17)
- Argiolas A, "Neuropeptides and central control of sexual behavior from the past to the present: a review", Progress Neurobiol, 108, pp.80~107 (2013)
- Díaz-Miranda L, "Localization of the heptapeptide GFSKLYFamide in the sea cucumber Holothuria glaberrima (Echinodermata): a light and electron microscopic study", J Comp Neurol, 352, pp.626~640 (1995)
- Erspamer V, "Structure and pharmacological actions of eledoisin, the active endecapeptide of the posterior salivary glands of Eledone", Experientia, 18, pp.58~59 (1962)
- Holmgren S, "Evolution of vertebrate neuropeptides", Brain Res Bull, 55, pp.723~735 (2001)
- Hoyle CH, "Neuropeptide families: evolutionary perspectives", Regul Pept, 73, pp.1~33 (1998)
- Iwakoshi E, "Peptide Chemistry 1994", pp.261~264 (1995), Protein Research Foundation
- Kanatani H, "Purification of gonad-stimulating substance obtained from radial nerves of starfish, Asterias amurensis", Dev Growth Differ, 13, pp.151~164 (1971)
- Kang KH, "Predation of Asteiras amurensis and Asterina pectinifera on valuable bivalves at different water temperature", Korean J Malacol, 16, pp.17~20 (2000)
- 김은정, "별불가사리(Asterina pectinifera)로부터 새로운 평활근 이환활성 펩타이드의 정제", 한국수산과학회지, 38(3), pp.148~152 (2005), 한국수산과학회
- Kim YS, "Selective feeding on the several bivalve mollusks by starfish, Asterias amurensis Lutken", Bull Fac Fish, 19, pp.244~249 (1969)
- Krieger DT, "Brain peptides: what, where, and why?", Science, 222, pp.975~985 (1983)
- Nässel DR, "Tachykinin-related peptides in invertebrates: a review", Peptides, 20, pp.141~158 (1999)
- Paine RT, "A short term experimental investigation of resource partitioning in a New Zealand rocky intertidal habitat", Ecology, 52, pp.1096~1106 (1971)
- Pfefferbaum A, "Human EEG response to beta-endorphin", Psychiatry Res, 1, pp.83~88 (1979)
- Schoofs L, "Locustatachykinin I and II, two novel insect neuropeptides with homology to peptides of the vertebrate tachykinin family", FEBS Lett, 26, pp.297~401 (1990)
- Semmens DC, "Discovery of a novel neurophysinassociated neuropeptide that triggers cardiac stomach contraction and retraction in starfish", J Exp Biol, 216, pp.4047~4053 (2013)
- Zadina JE, "Central nervous system effects of peptides, 1980-1985: a cross-listing of peptides and their central actions from the first six years of the journal Peptides", Peptides, 7, pp.497~537 (1986)