Isolation of a starfish myorelaxant peptide (SMP) isotype from the pyloric caeca of Patiria pectinifera
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Peptides are naturally occurring biological molecules that are found in all living organisms. These biologically active peptides play a key role in various biological processes. The aim of this study is the extraction and the purification of bioactive materials that induce relaxation of an apical muscle from the pyloric caeca of Patiria pectinifera. The acidified pyloric caeca extract was partially separated by the solid phase extraction using a stepwise gradient on Sep-Pak C18 cartridge. Among the fractions, materials eluted with 60% methanol/0.1% trifluoroacetic acid was put a thorough of a series of high performance liquid chromatography (HPLC) steps to isolate a neuropeptide with relaxation activity. The purified compound was eluted at 28% acetonitrile in 0.1% trifluoroacetic acid with retention time of 25.8 min on the CAPCELL-PAK C18 reversed-phase column. To determine the molecular weight and the amino acid sequence of the purified peptide, LC-MS and Edman degradation method were used, respectively. The primary structure of the peptide was determined to be FGMGGAYDPLSAGFTD which corresponded to the amino acid sequence of a starfish myorelaxant peptide (SMP) isotype (SMPb) found in the cDNA sequence encoding SMPa and its isotypes. In this study, a muscle relaxant neuropeptide (SMPb) has been isolated from pyloric caeca of starfish P. pectinifera. This is the first report of SMPb isolation on the protein level from P. pectinifera.
Abstract
Peptides are naturally occurring biological molecules that are found in all living organisms. These biologically active peptides play a key role in various biological processes. The aim of this study is the extraction and the purification of bioactive materials that induce relaxation of an apical muscle from the pyloric caeca of Patiria pectinifera. The acidified pyloric caeca extract was partially separated by the solid phase extraction using a stepwise gradient on Sep-Pak C18 cartridge. Among the fractions, materials eluted with 60% methanol/0.1% trifluoroacetic acid was put a thorough of a series of high performance liquid chromatography (HPLC) steps to isolate a neuropeptide with relaxation activity. The purified compound was eluted at 28% acetonitrile in 0.1% trifluoroacetic acid with retention time of 25.8 min on the CAPCELL-PAK C18 reversed-phase column. To determine the molecular weight and the amino acid sequence of the purified peptide, LC-MS and Edman degradation method were used, respectively. The primary structure of the peptide was determined to be FGMGGAYDPLSAGFTD which corresponded to the amino acid sequence of a starfish myorelaxant peptide (SMP) isotype (SMPb) found in the cDNA sequence encoding SMPa and its isotypes. In this study, a muscle relaxant neuropeptide (SMPb) has been isolated from pyloric caeca of starfish P. pectinifera. This is the first report of SMPb isolation on the protein level from P. pectinifera.
참고문헌 (31)
- Anderson JM, "Structure and function in the pyloric caeca of Asterias forbesi", Biol Bull Rev, 105, pp.47~61 (1953)
- Anderson JM, "Studies on the cardiac stomach of a starfish, Patiria miniata(Brandt)", Biol Bull Rev, 117, pp.185~201 (1959)
- Anderson JM, "Histological studies on the pyloric stomach and its appendages in Oreaster reticulatus (L.) (Asteroidea)", Biol Bull Rev, 156, pp.1~19 (1979)
- Chia FS, "Histology of the pyloric caeca and its changes during brooding and starvation in a starfish, Leptasterias hexactis", Biol Bull Rev, 136, pp.185~192 (1969)
- Davydov PV, "Animal species for developmental studies", pp.287~311 (1990), Springer
- Elphick MR, "NG peptides : a novel family of neurophysin-associated neuropeptides", Gene, 458, pp.20~26 (2010)
- Elphick MR, "The evolution and diversity of SALMFamide neuropeptides", PLOS ONE, 8, pp.e59076~ (2013)
- Elphick MR, "Distribution and action of SALMFamide neuropeptides in the starfish Asterias rubens", J Exp Biol, 198, pp.2519~2525 (1995)
- Elphick MR, "The SALMFamides : a new family of neuropeptides isolated from an echinoderm", Proc R Soc Lond B, 243, pp.121~127 (1991)
- Gruber CW, "Physiology of invertebrate oxytocin and vasopressin neuropeptides", Exp Physiol, 99, pp.55~61 (2014)
- Hook V, "Proteases for processing proneuropeptides into peptide neurotransmitters and hormones", Annu Rev Pharmacol Toxicol, 48, pp.393~423 (2008)
- Ji C, "Echinoderms have bilateral tendencies", PLOS ONE, 7, pp.e28978~ (2012)
- Jo MJ, "Purification and characterization of neuropeptides from starfish, Asterias amurensis", Ph.D, pp.2019
- Kim CH, "Identification of evolutionarily conserved residues required for the bioactivity of a pedal peptide", orcokinin-type neuropeptide, 2018, pp.103, 10~18
- Kim CH, "Identification of a novel starfish neuropeptide that acts as a muscle relaxant", J Neurochem, 137, pp.33~45 (2016)
- Lin M, "Pedal peptide", orcokinin-type neuropeptide signaling in a deuterostome : the anatomy and pharmacology of starfish myorelaxant peptide in Asterias rubens, 2017, pp.525, 3890~3917
- Mah CL, "Global diversity and phylogeny of the Asteroidea(Echinodermata)", PLOS ONE, 7, pp.e35644~ (2012)
- Martindale MQ, "The development of radial and biradial symmetry : the evolution of bilaterality", Am Zool, 38, pp.672~684 (1998)
- Martinez A, "Microscopic study of the pyloric caeca of the starfish Marthasterias glacialis(Echinodermata) : finding of endocrine cells", J Morphol, 202, pp.151~164 (1989)
- Merkler DJ, "C-terminal amidated peptides : production by the in vitro enzymatic amidation of glycine-extended peptides and the importance of the amide to bioactivity", Enzyme Microb Technol, 16, pp.450~456 (1994)
- Melarange R, "SALMFamide neuropeptides cause relaxation and eversion of the cardiac stomach in starfish", Proc R Soc Lond B, 266, pp.1785~1789 (1999)
- Odekunle EA, "Ancient role of vasopressin", oxytocin-type neuropeptides as regulators of feeding revealed in an echinoderm, 2019, pp.17, 60~
- Pawson DL, "Phylum Echinodermata", Zootaxa, 1668, pp.749~764 (2007)
- Rowe ML, "The neuropeptide transcriptome of a model echinoderm, the sea urchin Strongylocentrotus purpuratus", Gen Comp Endocrinol, 179, pp.331~344 (2012)
- Russo AF, "Overview of neuropeptides : awakening the senses", Headache, 57, pp.37~46 (2017)
- Semmens DC, "Discovery of a novel neurophysin-associated neuropeptide that triggers cardiac stomach contraction and retraction in starfish", J Exp Biol, 216, pp.4047~4053 (2013)
- Semmens DC, "Transcriptomic identification of starfish neuropeptide precursors yields new insights into neuropeptide evolution", Open Biol, 6, pp.150224~ (2016)
- Smith MK, "The neuropeptidome of the crown-of-thorns starfish, Acanthaster planci", J Proteom, 165, pp.61~68 (2017)
- Stangier J, "Orcokinin : a novel myotropic peptide from the nervous system of the crayfish, Orconectes limosus", Peptides, 13, pp.859~864 (1992)
- Tinoco AB, "Characterization of NGFFYamide signaling in starfish reveals roles in regulation of feeding behavior and locomotory systems", Front Endocrinol, 9, pp.507~ (2018)
- Wang P, "A review of pedal peptide", orcokinin-type neuropeptides, 2021, pp.22, 41~49