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별 불가사리(Asterina pectinifera) 및 아므르 불가사리(Asterias amurensis)추출물의 항균, 항산화 활성 및 미백 효과

The Antimicrobial, Antioxidant, and Tyrosinase Inhibitory Activities of Solvent Extracts of Asterina pectinifera and Asterias amuren

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초록

This study examined the antimicrobial, antioxidant, and tyrosinase inhibitory activities of bioactive compounds extracted from two starfish, Asterina pectinifera and Asterias amurensis, using solvent extraction after Protamex™ hydrolysis. Methanol and acetone fractions collected by stepwise extraction from specimens were subjected to silica gel column chromatography (SGCC) (200 mesh and 400 mesh), followed by high-performance liquid chromatography (HPLC). Two fractions (7:3 and 5:5 chloroform : methanol ratio, v/v) eluted using silica gel column chromatography from the two starfishes showed higher antimicrobial activity against Propionibacterium acnes and dermatophyte fungi (Epidermophyton floccosum, Microsporum audouinii, Trichophyton ferrugineum, Trichophyton mentagrophytes, and Trichophyton rubrum), antioxidant activity (EDA50, mg/mL), and tyrosinase inhibitory activity compared to the other fractions. The final fractions obtained from Asterina pectinifera (RT 7.53, 8.93, and 10.48 min) and Asterias amurensis (RT 5.02 min) by SGCC (400 mesh) and HPLC from two SGCC fractions (200 mesh) showed 8.94 and 15.59 mg/mL antioxidant activity (EDA50) and 46.89 and 40.19 % tyrosinase inhibitory activity, respectively. Extracts from starfishes are potential cosmetic basic material.

Abstract

This study examined the antimicrobial, antioxidant, and tyrosinase inhibitory activities of bioactive compounds extracted from two starfish, Asterina pectinifera and Asterias amurensis, using solvent extraction after Protamex™ hydrolysis. Methanol and acetone fractions collected by stepwise extraction from specimens were subjected to silica gel column chromatography (SGCC) (200 mesh and 400 mesh), followed by high-performance liquid chromatography (HPLC). Two fractions (7:3 and 5:5 chloroform : methanol ratio, v/v) eluted using silica gel column chromatography from the two starfishes showed higher antimicrobial activity against Propionibacterium acnes and dermatophyte fungi (Epidermophyton floccosum, Microsporum audouinii, Trichophyton ferrugineum, Trichophyton mentagrophytes, and Trichophyton rubrum), antioxidant activity (EDA50, mg/mL), and tyrosinase inhibitory activity compared to the other fractions. The final fractions obtained from Asterina pectinifera (RT 7.53, 8.93, and 10.48 min) and Asterias amurensis (RT 5.02 min) by SGCC (400 mesh) and HPLC from two SGCC fractions (200 mesh) showed 8.94 and 15.59 mg/mL antioxidant activity (EDA50) and 46.89 and 40.19 % tyrosinase inhibitory activity, respectively. Extracts from starfishes are potential cosmetic basic material.

참고문헌 (24)

  1. Blois MS, "Antioxidant determination by the use of a stable free radical", Nature, 4617, pp.1199~1200 (1958)
  2. Chen QX, "Kinetics of mushroom tyrosinase inhibition by quercetin", J Agric Food Chem, 50, pp.4108~4112 (2002)[DOI]
  3. Cho SY, "Screening for antioxidant activity of edible plants", J Korean Soc Food Sci Nutr, 30, pp.133~137 (2001)
  4. Cho SY, "Screening for antimicrobial compounds in unused marine resources by the paper disk method", Korean J Food Sci Technol, 26, pp.261~265 (1994)
  5. Cho SY, "Screening for the antioxidants in unused marine resources by the polarographic method", Korean J Food Sci Technol, 26, pp.417~421 (1994)
  6. Cho SY, "Separation and identification of nature antimicrobial agent from starfish", Paper presented at 47th Conference of Korean Soc Food Sci Nutr (2002), Kangwon National University
  7. Cho SY, "Separation and identification of nature antioxidative agent from starfish and sea urchin integument", Paper presented at 47th Conference of Korean Soc Food Sci Nutr (2002), Kangwon National University
  8. Choi DH., "Studies on the screening and chemical properties of antifungal compound from Asternia sp", 석사, pp.1993
  9. Choi DH, "Characterization of antimicrobial agents extracted from Asterina pectinifera", International J Antimicrobial Agent, 11, pp.65~68 (1999)
  10. Choi SM, "Screening of the antibacterial activity of natural products against Propionibacterium acnes", Yakhak Hoeji, 42, pp.89~94 (1998)
  11. Choi SM, "Screening of 5 α-reductase inhibition and comedolytic effects from natural products", Yakhak Hoeji, 43, pp.342~350 (1999)
  12. Findlay JA, "On the saponins of the starfish Asterias vulgaris", J Nat Prod, 47, pp.113~116 (1984)
  13. Higashi H, "Studies on utilization of worthless marine animals for feed-I. : Production of starfish solubles for feed", Bull Japanese Soc Sci Fish, 21, pp.271~279 (1955)
  14. Ji HG, "The study for efficacy, effect and stabilization of trichosanthes kirilowii root, prunella vulgaris leaf and clematis chinensis root as a new whitening ingredients", J Soc Cosmet Scientists Korea, 30, pp.123~128 (2004)
  15. Jung YJ., "Characterization of bioactive compounds obtained from starfishes", 석사, pp.2002
  16. Kang KH, "Predation of Asteiras amurensis and Asterina pectinifera on valuable bivalves at different water temperature", Korean J Malacol, 16, pp.17~20 (2000)
  17. Kim JY, "Synthesis of novel kojic acid derivatives and their tyrosinase inhibitory activities", Yakhak Hoeji, 43, pp.28~32 (1999)
  18. Kishimura H, "Free amino acids of starfish", Nippon Suisan Gakkaishi, 56, pp.1693~ (1990)
  19. Lee CK, "Chemical components of starfish and their availability for fertilizer", Technical report of Fish Res Dev Agency, 77, pp.57~74 (1989)
  20. Park HY, "Development of industrialization technology with starfish", Food Indust Nutr, 8, pp.18~22 (2003)
  21. Park SW, "A Study on the development of the extermination gear for starfish, Asterias amurensis and its efficiency", Bull Korean Fish Tech Soc, 33, pp.166~172 (1997)
  22. Rau W, "Mechanism of photoregulation of carotenoid biosynthetic in plants", Pure Appl Chem, 57, pp.777~784 (1985)
  23. Seo JK., "Conformation and biological activity of Mastoparan B and its analogs: Isolation and characterization of the biological substances from Inshore Hagfish (Eptatretus burgeri) skin and starfish (Asterina pectinifera)", 석사, pp.1997
  24. Song YH., "Purification and Characterization of new Lectins from Asterina pectinifera", 석사, pp.1992

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