귀전우, 백화사설초, 와송 추출물을 처치한 난소암과 자궁경부암 세포에서의 단백질 발현 변화
Altered Protein Expression in Ovarian and Cervical Cancer Cells by the Treatment of Extracts from Euonymus alatus Sieb, Oldenlandia diffusa (Willd.) Roxburgh, and Orostachys japonicus A. Berger
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Background : Despite recent advances in cancer management, prognosis of ovarian cancer is poor. Anticancer effects of herbal medicine, such as Euonymus alatus Sieb, Oldenlandia diffusa (Willd.) Roxburgh, and Orostachys japonicus A. Berger, have been reported in treatment of ovarian and cervical cancers, but the systematic approaches to explain their molecular mechanism(s) have not yet been established. Objectives : To establish a basis of understanding for anti-cancer mechanisms of herbal medicine, we profiled protein expression in human ovarian and cervical cancer cells treated with the extracts from Euonymus alatus Sieb, Oldenlandia diffusa (Willd.) Roxburgh and Orostachys japonicus A. Berger. Methods : Human ovarian cancer cell line NIH:OVCAR-3, and human cervical cancer cell line HeLa were employed in the present study. Whole protein was obtained from the cells harvested at 48 hours after the treatment with herbal water-extract, and analyzed by 2DE-based proteomic approach. Results : Various changes of protein expression induced by the herbal treatment were monitored : down-regulation of molecular chaperone (calreticulin variant), glycolytic enzymes (D-3-phosphoglycerate dehydrogenase, glyceraldehyde 3-phosphate dehydrogenase and alpha-enolase), RNA processing molecules (hnRNP A2/B1), and antioxidant protein (peroxiredoxin 1). Conclusions : Repression of glycolysis has been accepted as the mechanism to increase anticancer reagent's effect. Thus, down-regulation of glycolytic enzymes by the herbal extracts suggested a possible synergistic effect of herbs in the presence of platinum-based therapeutics. In further study, as well as the synergistic effect of the herbs, it has to be further validated whether artificial regulation of hnRNP A2/B1 in ovarian cancer cells affects various cancer survival factors, since RNA processing can be interrupted by deranged expression of hnRNP subtypes, and it results in an inhibition of cancer cell growth.
Abstract
Background : Despite recent advances in cancer management, prognosis of ovarian cancer is poor. Anticancer effects of herbal medicine, such as Euonymus alatus Sieb, Oldenlandia diffusa (Willd.) Roxburgh, and Orostachys japonicus A. Berger, have been reported in treatment of ovarian and cervical cancers, but the systematic approaches to explain their molecular mechanism(s) have not yet been established. Objectives : To establish a basis of understanding for anti-cancer mechanisms of herbal medicine, we profiled protein expression in human ovarian and cervical cancer cells treated with the extracts from Euonymus alatus Sieb, Oldenlandia diffusa (Willd.) Roxburgh and Orostachys japonicus A. Berger. Methods : Human ovarian cancer cell line NIH:OVCAR-3, and human cervical cancer cell line HeLa were employed in the present study. Whole protein was obtained from the cells harvested at 48 hours after the treatment with herbal water-extract, and analyzed by 2DE-based proteomic approach. Results : Various changes of protein expression induced by the herbal treatment were monitored : down-regulation of molecular chaperone (calreticulin variant), glycolytic enzymes (D-3-phosphoglycerate dehydrogenase, glyceraldehyde 3-phosphate dehydrogenase and alpha-enolase), RNA processing molecules (hnRNP A2/B1), and antioxidant protein (peroxiredoxin 1). Conclusions : Repression of glycolysis has been accepted as the mechanism to increase anticancer reagent's effect. Thus, down-regulation of glycolytic enzymes by the herbal extracts suggested a possible synergistic effect of herbs in the presence of platinum-based therapeutics. In further study, as well as the synergistic effect of the herbs, it has to be further validated whether artificial regulation of hnRNP A2/B1 in ovarian cancer cells affects various cancer survival factors, since RNA processing can be interrupted by deranged expression of hnRNP subtypes, and it results in an inhibition of cancer cell growth.
참고문헌 (25)
- 김정인, "Inhibitory activity of Euonymus alatus against alpha-glucosidase in vitro and in vivo", Nutrition Research and Practice, 1(3), pp.184~188 (2007), 한국영양학회
- Hwang JS, "Development of T Cell Immunomodulators from Natural Products", Research Focus on Natural Products and the Body's Immune and Inflammatory, pp.253~273 (2007)
- Deok-Seon Ryu, "Effects of polysaccharides derived from Orostachys japonicus on induction of cell cycle arrest and apoptotic cell death in human colon cancer cells", BMB reports, 43(11), pp.750~755 (2010), 생화학분자생물학회
- Sandra T, "Membrane proteins and membrane proteomics", membrane proteomics, 8(19), pp.3924~3932 (2008)
- 류원정, "퇴행성 뇌질환 진단을 위한 프로테오믹스와 단백질 칩 분석의 응용", 대한신경과학회지, 21(6), pp.3~599 (2003), 대한신경과학회
- Wen JK, "Application of genomics and proteomics in study of traditional Chinese medicine", Zhong Xi Yi Jie He Xue Bao, 2(5), pp.323~325 (2004)
- Cho WC, "Application of proteomics in Chinese medicine research", Am J Chin Med, 35(6), pp.911~922 (2007)
- Kang YJ, "Herbogenomics: from traditional Chinese medicine to novel therapeutics", Exp Biol Med, 233(9), pp.1059~1065 (2008)
- Liu CB, "The role of traditional Chinese medicine in colorectal cancer treatment", Tech Coloproctol, 12(1), pp.1~6 (2008)
- 신승용(申昇容), "？문법？ 敎科書 內容 記述上의 몇 問題와 改善 方向", 어문연구(語文硏究), 34(4), pp.401~426 (2006), 한국어문교육연구회
- Can CY, "Apoptotic B16-F1 cells coated with recombinant calreticulin mediated anti-tumor immune response in mice", Chinese Journal of Cancer Research, 22(4), pp.253~259 (2010)
- Kepp O, "The immunogenicity of tumor cell death", Curr Opin Oncol, 21(1), pp.71~76 (2009)
- Chen CN, "Identification of calreticulin as a prognosis marker and angiogenic regulator in human gastric cancer", Ann Surg Oncol, 16(2), pp.524~533 (2009)
- Panaretakis T, "EMBO J", Mechanisms of pre-apoptotic calreticulin exposure in immunogenic cell death, 28(5), pp.578~590 (2009)
- Lucia C, "Comparative Proteomic Analysis of Paclitaxel Sensitive A2780 Epithelial Ovarian Cancer Cell Line and Its Resistant Counterpart A2780TC1 by 2D-DIGE: The Role of ERp57", J. Proteome Res, 8(4), pp.1902~1912 (2009)
- Wei W, "Nuclear proteome analysis of cisplatin-treated HeLa cells", Mutation Research, 2010, pp.691(2), 1~8
- Ganapathy-Kanniappan S, "Glyceraldehyde-3-phosphate dehydrogenase(GAPDH) is pyruvylated during 3-bromopyruvate mediated cancer cell death", Anticancer Res, 29(12), pp.4909~4918 (2009)
- Shih NY, "Anti-alpha -enolase autoantibodies are down-regulated in advanced cancer patients", Jpn J Clin Oncol, 40(7), pp.663~669 (2010)
- Ziprem Y, "MicroRNA 17", 20 inhibits cellular inbasion and tumor metastasis in breast cancer by heterotypic signaling, 2010, pp.107(18), 8231~8236
- Valera VA, "Protein expression profiling in the spectrum of renal cell carcinomas", J Cancer, 1, pp.184~196 (2010)
- Guha M, "nuclear ribonucleoprotein A2 is a common transcriptional coactivator in the nuclear transcription response to mitochondrial respiratory stress", Mol Biol Cell, 20(18), pp.4107~4119 (2009)
- Markus HH, "A Common Pathway for All Autoimmune Diseases? The Unholy Alliance of Environment, Cell Death and Nucleic Acids", Current Immunology Reviews, 5, pp.69~88 (2009)
- Wang X, "Selective association of peroxiredoxin 1 with genomic DNA and COX-2 upstream promoter elements in estrogen receptor negative breast cancer cells", Mol Biol Cell, 21(17), pp.2987~2995 (2010)
- Chhipa RR, "Prx1 Enhances Androgen Receptor Function in Prostate Cancer Cells by Increasing Receptor Affinity to Dihydrotestosterone", MOLECULAR CANCER RESEARCH, 7(9), pp.1543~1552 (2009), AMER ASSOC CANCER RESEARCH
- Joshi G, "Alterations in brain antioxidant enzymes and redox proteomic identification of oxidized brain proteins induced by the anti-cancer drug adriamycin: implications for oxidative stress- mediated chemobrain", Neuroscience, 166(3), pp.796~807 (2010)