사육수의 pH변화가 숭어(Mugil cephalus)에 미치는 생리적 영향
Physiological Responses of Gray Mullet Mugil cephalus to Low-pH Water
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
We examined changes in the physiological responses of gray mullet Mugil cephalus exposed to acidic seawater (pH 6.0, 6.5, 7.0) and normal seawater (pH 8.0, control) for 15 days. As pH decreased, survival rate and body weight also decreased. Levels of aminotransferase, total protein and triglycerides also differed significantly with changes in pH, presumably due to stress caused by exposure to acidic water. The level of osmotic pressure was significantly higher in the pH 6.0 group than in other groups. Superoxide dismutase was significantly higher in the pH 6.5 and 7.0 groups than in the pH 8.0 group, and glutathione level was lowest in the pH 6.0 group. We conclude that decreasing the pH level of seawater induces a stress response in fish, damaging their ability to control their hematological and osmotic pressure. Antioxidant enzymes are generally sensitive to osmotic stress; in this study, antioxidant activity significantly changed with pH level. These results indicate that physiological stress induced by exposure to acidification reduces survival rates and inhibits growth in M. cephalus.
Abstract
We examined changes in the physiological responses of gray mullet Mugil cephalus exposed to acidic seawater (pH 6.0, 6.5, 7.0) and normal seawater (pH 8.0, control) for 15 days. As pH decreased, survival rate and body weight also decreased. Levels of aminotransferase, total protein and triglycerides also differed significantly with changes in pH, presumably due to stress caused by exposure to acidic water. The level of osmotic pressure was significantly higher in the pH 6.0 group than in other groups. Superoxide dismutase was significantly higher in the pH 6.5 and 7.0 groups than in the pH 8.0 group, and glutathione level was lowest in the pH 6.0 group. We conclude that decreasing the pH level of seawater induces a stress response in fish, damaging their ability to control their hematological and osmotic pressure. Antioxidant enzymes are generally sensitive to osmotic stress; in this study, antioxidant activity significantly changed with pH level. These results indicate that physiological stress induced by exposure to acidification reduces survival rates and inhibits growth in M. cephalus.
참고문헌 (37)
- ArasHisar Ş, "Inhibitory effects of ammonia and urea on gill carbonic anhydrase enzyme activity of rainbow trout(Oncorhynchus mykiss)", Environ Toxicol Pharmacol, 17, pp.125~128 (2004)
- Carbon cycle: Marine manipulations. Nature Reports Climate Change, "Arrigo KR.", 100~101 (2007)
- Anders Aksnes, "Catalase, glutathione peroxidase and superoxide dismutase in different fish species", Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 69(4), pp.893~896 (1981), Elsevier BV[DOI]
- Bonga SW, "The stress response in fish", Physiol Rev, 77, pp.591~625 (1997)
- ANTHONY CALABRESE, "THE pH TOLERANCE OF EMBRYOS AND LARVAE OF MERCENARIA MERCENARIA AND CRASSOSTREA VIRGINICA", The Biological Bulletin, 131(3), pp.427~436 (1966), University of Chicago Press[DOI]
- Chun-Yao Chen, "Comparative blood chemistry and histopathology of tilapia infected with Vibrio vulnificus or Streptococcus iniae or exposed to carbon tetrachloride, gentamicin, or copper sulfate", Aquaculture, 239(1-4), pp.421~443 (2004), Elsevier BV[DOI]
- David A. Dalton, "Correlations between the ascorbate-glutathione pathway and effectiveness in legume root nodules", Physiologia Plantarum, 87(3), pp.365~370 (1993), Wiley[DOI]
- Danielle L. Dixson, "Odor tracking in sharks is reduced under future ocean acidification conditions", Global Change Biology, 21(4), pp.1454~1462 (2015), Wiley[DOI]
- Scott C. Doney, "Ocean acidification: the other CO2 problem", Annual Review of Marine Science, 1(1), pp.169~192 (2009), Annual Reviews[DOI]
- Forman HJ, "On the stability of bovine superoxide dismutase the effects of metals", J Biol Chem, 248, pp.2645~2649 (1973)
- T. Gabryelak, "Seasonal variations in the activities of peroxide metabolism enzymes in erythrocytes of freshwater fish species", Comparative Biochemistry and Physiology Part C: Comparative Pharmacology, 75(2), pp.383~385 (1983), Elsevier BV[DOI]
- Jean-Pierre Gattuso, "Ocean biogeochemistry:calcification and CO2", Nature, 407(6802), pp.311~313 (2000), Springer Nature[DOI]
- Gattuso JP, "Ocean acidification" (2011), Oxford university press
- Jon Arne Grøttum, "Acute toxicity of carbon dioxide on European seabass (Dicentrarchus labrax): Mortality and effects on plasma ions", Comparative Biochemistry and Physiology Part A: Physiology, 115(4), pp.323~327 (1996), Elsevier BV[DOI]
- T. Hannedouche, "Feedback-mediated reduction in glomerular filtration during acetazolamide infusion in insulin-dependent diabetic patients", Clinical Science, 81(s25), pp.457~464 (1991), Portland Press Ltd.[DOI]
- M. Kaur, "Heat stress-induced alterations of antioxidants in the freshwater fish Channa punctata Bloch", Journal of Fish Biology, 67(6), pp.1653~1665 (2005), Wiley[DOI]
- T. Kikkawa, "Acute CO2 tolerance during the early developmental stages of four marine teleosts", Environmental Toxicology, 18(6), pp.375~382 (2003), Wiley[DOI]
- Takashi Kikkawa, "Comparison of the lethal effect of CO2 and acidification on red sea bream (Pagrus major) during the early developmental stages", Marine Pollution Bulletin, 48(1-2), pp.108~110 (2004), Elsevier BV[DOI]
- 김이경, "여윔증 넙치, Paralichthys olivaceus의 증상에 대한 병태생리학적 고찰", 한국어병학회지, 24(1), pp.11~18 (2011), 한국어병학회
- Jon Knutzen, "Effects of decreased pH on marine organisms", Marine Pollution Bulletin, 12(1), pp.25~29 (1981), Elsevier BV[DOI]
- Yukimasa KUWATANI, "Effects of pH of Culture Water on the Growth of the Japanese Pearl Oyster", NIPPON SUISAN GAKKAISHI, 35(4), pp.342~350 (1969), Japanese Society of Fisheries Science[DOI]
- Lai F, "Altered neurotransmitter function in CO2-exposed stickleback (Gasterosteus aculeatus):a temperate model species for ocean acidification research", Conserv Physiol, 3, pp.cov018~ (2015)[DOI]
- Øyvind Lie, "Growth and chemical composition of adult Atlantic salmon (Salmo salar) fed dry and silage-based diets", Aquaculture, 69(3-4), pp.343~353 (1988), Elsevier BV[DOI]
- McDonald DG, "Branchial and renal acid and ion fluxes in the rainbow trout, Salnro guirdneri, at low environmentaI pH", J Exp Biol, 93, pp.181~118 (1981)
- Molander DW, "Serum glutamic oxalacetic transaminase as an index of hepatocellular integrity", J Lab Clin Med, 46, pp.831~ (1955)
- Michelle Ou, "Responses of pink salmon to CO2-induced aquatic acidification", Nature Climate Change, 5(10), pp.950~955 (2015), Springer Nature[DOI]
- M.S. Parihar, "Changes in lipid peroxidation, superoxide dismutase activity, ascorbic acid and phospholipid content in liver of freshwater catfish Heteropneustes fossilis exposed to elevated temperature", Journal of Thermal Biology, 21(5-6), pp.323~330 (1996), Elsevier BV[DOI]
- M.S. Parihar, "Responses of superoxide dismutase, glutathione peroxidase and reduced glutathione antioxidant defenses in gills of the freshwater catfish (Heteropneustes fossilis) to short-term elevated temperature", Journal of Thermal Biology, 22(2), pp.151~156 (1997), Elsevier BV[DOI]
- Pörtner HO, "Acid-base regulation, metabolism and energetics in Sipunculus nudusas a function of ambient carbon dioxide level", J Exp Biol, 201, pp.43~55 (1998)
- Hans O. Pörtner, "Biological Impact of Elevated Ocean CO2 Concentrations: Lessons from Animal Physiology and Earth History", Journal of Oceanography, 60(4), pp.705~718 (2004), Springer Nature[DOI]
- Hélène Roche, "Fish blood parameters as a potential tool for identification of stress caused by environmental factors and chemical intoxication", Marine Environmental Research, 41(1), pp.27~43 (1996), Elsevier BV[DOI]
- Richard L. Saunders, "Effects of low environmental pH on smolting of Atlantic salmon (Salmo salar)", Canadian Journal of Fisheries and Aquatic Sciences, 40(8), pp.1203~1211 (1983), Canadian Science Publishing[DOI]
- Carl B. Schreck, "Stress and rearing of salmonids", Aquaculture, 28(1-2), pp.241~249 (1982), Elsevier BV[DOI]
- Climate change 2007:The physical science basis: contribution of Working Group I to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change, "Solomon S", Cambridge University Press, 97~ (2007)
- Winston GW, "Prooxidant and antioxidant mechanisms in aquatic organisms", Aquat Toxicol, 19, pp.137~161 (1991)
- Graham Young, "Smoltification and seawater adaptation in coho salmon (Oncorhynchus kisutch): Plasma prolactin, growth hormone, thyroid hormones, and cortisol", General and Comparative Endocrinology, 74(3), pp.335~345 (1989), Elsevier BV[DOI]
- Jingfei Zhang, "Effects of chronic exposure of 2,4-dichlorophenol on the antioxidant system in liver of freshwater fish Carassius auratus", Chemosphere, 55(2), pp.167~174 (2004), Elsevier BV[DOI]