본문 바로가기
KERD
논문KCI 등재

Signal Transduction of Eel Luteinizing Hormone Receptor (eelLHR) and Follicle Stimulating Hormone Receptor (eelFSHR) by Recombinant Equine Chorionic Gonadotropin (rec-eCG) and Native eCG

2

조회

0

다운로드

4

인용

0

참고문헌

키워드

주제분류생물학

초록

Previous studies showed that recombinant equine chorionic gonadotropin (rec-eCGβ/α) exhibits both follicle-stimulating hormone (FSH) and luteinizing hormone (LH)-like activities in rat LHR- and FSHR-expressing cells. In this study, we analyzed signal transduction by eelFSHR and eelLHR upon stimulation with rec-eCGβ/α and native eCG. The cyclic adenosine monophosphate (cAMP) stimulation in CHO-K1 cells expressing eelLHR was determined upon exposure to differ-ent doses (0–1,450 ng/mL) of rec-eCGβ/α and native eCG. The EC50 values of rec-eCGβ/α and native eCG were 172.4 and 786.6 ng/mL, respectively. The activity of rec-eCGβ/α was higher than that of native eCG. However, signal transduction in the CHO PathHunter Parental cells expressing eelFSHR was not enhanced by stimulation with both agonist rec-eCGβ/α and native eCG. We concluded that rec-eCGβ/α and native eCG were completely active in cells expressing eelLHR, similar to the activity in the mammalian cells expressing LHRs. However, rec-eCGβ/α and native eCG did not invoke any signaling re-sponse in the cells expressing eelFSHR. These results suggest that eCG has a potent activity in cells expressing eelLHR. Thus, we also suggest that rec-eCGβ/α can induce eel maturation by administering gonadotropic reagents (LH), such as salmon pitu-itary extract.

Abstract

Previous studies showed that recombinant equine chorionic gonadotropin (rec-eCGβ/α) exhibits both follicle-stimulating hormone (FSH) and luteinizing hormone (LH)-like activities in rat LHR- and FSHR-expressing cells. In this study, we analyzed signal transduction by eelFSHR and eelLHR upon stimulation with rec-eCGβ/α and native eCG. The cyclic adenosine monophosphate (cAMP) stimulation in CHO-K1 cells expressing eelLHR was determined upon exposure to differ-ent doses (0–1,450 ng/mL) of rec-eCGβ/α and native eCG. The EC50 values of rec-eCGβ/α and native eCG were 172.4 and 786.6 ng/mL, respectively. The activity of rec-eCGβ/α was higher than that of native eCG. However, signal transduction in the CHO PathHunter Parental cells expressing eelFSHR was not enhanced by stimulation with both agonist rec-eCGβ/α and native eCG. We concluded that rec-eCGβ/α and native eCG were completely active in cells expressing eelLHR, similar to the activity in the mammalian cells expressing LHRs. However, rec-eCGβ/α and native eCG did not invoke any signaling re-sponse in the cells expressing eelFSHR. These results suggest that eCG has a potent activity in cells expressing eelLHR. Thus, we also suggest that rec-eCGβ/α can induce eel maturation by administering gonadotropic reagents (LH), such as salmon pitu-itary extract.

참고문헌 (25)

  1. Aggarwal BB, "Effects of histidine modification on the biological and immunological activities of equine chorionic gonadotropin", Arch Biochem Biophys, 202, pp.121~125 (1980)[DOI]
  2. Chopineau M, "<TEX>${\beta}$<", TEX>-Subunit 102-104 residues are crucial to confer FSH activity to equine LH, J Endocrinol, pp.2001, 169[DOI]
  3. Cohen L, "The recombinant equine <TEX>$LH{\beta}$<", TEX> subunit combines divergent intracellular traits human <TEX>$LH{\beta}$<, TEX> subunits, pp.Theriogenology, 2015[DOI]
  4. Galet C, "The <TEX>${\beta}104-109$<", TEX> sequence is essential for the secretion of correctly folded single-chain <TEX>${\beta}{\alpha}$<, CG and for its FSH activity, pp.J Endocrinol, 2009[DOI]
  5. Guillou F, "Purification of equine gonadotropins and comparative study of their aciddissociation and receptor-binding specificity", Biochim Biophys Acta, 755, pp.229~236 (1983)[DOI]
  6. Jeoung YH, "Biological functions of the COOH-terminal amino acids of the <TEX>${\alpha}$<", TEX>-subunit of tethered equine chorionic gonadotropin, 2010, pp.34, 47~53
  7. Kagawa H, "Effects of rearing period in seawater on induced maturation in female Japanese eel Anguilla japonica", Fish Sci, 64, pp.77~82 (1998)[DOI]
  8. Kazeto Y, "Japanese eel FSH and luteinizing hormone (LH): Production of biologically active recombinant FSH and LH by Drosophila S2 cells and their differential actions on the reproductive biology", Biol Reprod, 79, pp.938~946 (2008)[DOI]
  9. Kim DJ, "Data on the characterization of folliclestimulating hormone monoclonal antibodies and localization in Japanese eel pituitary", Data Brief, 8, pp.404~410 (2016)[DOI]
  10. Kim DJ, "Production and characterization of monoclonal antibodies against recombinant tethered follicle-stimulating hormone from Japanese eel Anguilla japonica", Gem Comp Endocrinol, 233, pp.8~15 (2016)[DOI]
  11. Kobayashi M, "Production of recombinant Japanese eel gonadotropins by baculovirus in silkworm larvae", Gen Comp Endocrionol, 167, pp.379~386 (2010)[DOI]
  12. 이소연, "Biochemical Characterization of Recombinant Equine Chorionic Gonadotropin (rec-eCG), Using CHO Cells and PathHunter Parental Cells Expressing Equine Luteinizing Hormone", Chorionic Gonadotropin Receptors (eLH, 생명과학회지, pp.2017, 27(8)[DOI]
  13. Legardinier S, "Biological activities of recombinant equine luteinizing hormone", chorionicgona dotropin (eLH, J Mol Endocrinol, pp.2005, 34[DOI]
  14. Legardinier S, "Mammalian-like nonsialyl complextype N-glycosylation of equine gonadotropins in <TEX>$Mimic^{TM}$<", TEX> insect cells, 2005, pp.15, 776~790
  15. Legardinier S, "Stability and biological activities of heterodimeric and single-chain equine LH", chorionic gonadotropin variants, 2008, pp.40, 185~198
  16. Min KS, "Site-directed mutagenesis of recombinant equine chorionic gonadotropin", luteinizing hormone: Differential role of oligosaccharides in luteinizing hormone- and follicle-stimulating hormone-like activities, 1996, pp.43, 585~593
  17. Min KS, "Biological activities of tethered equine chorionic gonadotropin (eCG) and its deglycosylated mutants", J Reprod Dev, 50, pp.297~304 (2004)[DOI]
  18. Moore WT, "Pregnant mare serum gonadotropin", J Biol Chem, 250, pp.6930~6936 (1980)
  19. Murphy BD, "Equine chorionic gonadotropin", Endocr Rev, 12, pp.27~44 (1991)[DOI]
  20. Ohta H, "Follicle-stimulating hormone induces spermatogenesis mediated by androgen production in Japanese eel, Anguilla japonica", Biol Reprod, 77, pp.970~977 (2007)[DOI]
  21. Ohta H, "Changes in milt volume and sperm quality with time after an injection of recombinant Japanese eel luteinizing hormone in male Japanese eels", Aquaculture, 479, pp.150~154 (2017)[DOI]
  22. Jong-Ju Park, "Function of the Tethered rec-eCG in Rat and Equine Receptors", Reproductive & Developmental biology, 33(4), pp.229~236 (2009), 사단법인 한국동물생명공학회
  23. Jong-Ju Park, "β-Subunit 94∼96 Residues of Tethered Recombinant Equine Chorionic Gonadotropin are Important Sites for Luteinizing Hormone and Follicle Stimulating Hormone like Activities", Reproductive & Developmental biology, 34(1), pp.33~40 (2010), 사단법인 한국동물생명공학회
  24. 박종주, "Internalization of Rat FSH and LH", CG Receptors by rec-eCG in CHO-K1 Cells, 2017, pp.21(2), 111~120[DOI]
  25. Stewart F, "Biological functions and receptor binding activities of equine chorionic gonadotrophins", J Reprod Ferti, 62, pp.527~536 (1981)[DOI]

관련 논문AI추천

Signal Transduction of Constitutive Activating and Inactivating Eel Lutropin/ Choriogonadotropin Receptor (Eel LH/ CGR) Mutants by Recombinant Equine Chorionic Gonadotropin (Rec-eCG)민관식, 박재혁, 정하린, Munkhzaya Byambaragchaa, Sei Hyen Park한국발생생물학회 · 발생과 생식2024 · 제28권 제4호The C-terminal Phosphorylation Sites of eel Follicle-Stimulating Hormone Receptor are Important Role in the Signal Transduction강명화, 민관식, Jeong-Min Kim, Munkhzaya Byambaragchaa한국발생생물학회 · 발생과 생식2018 · 제22권 제2호Constitutive Activating Eel Luteinizing Hormone Receptors Induce Constitutively Signal Transduction and Inactivating Mutants Impair Biological Activity민관식, Dong-Wan Kim, Munkhzaya Byambaragchaa, Seung-Hee Choi한국발생생물학회 · 발생과 생식2021 · 제25권 제3호Internalization of Rat FSH and LH/CG Receptors by rec-eCG in CHO-K1 Cells강명화, 김정수, 민관식, 박종주, 성훈기, Byambaragchaa Munkhzaya한국발생생물학회 · 발생과 생식2017 · 제21권 제2호Characterization of ovarian culture in vitro and sex steroids in vivo by recombinant eel gonadotropin treatments in the eel Anguilla japonica김대중, 김신권, 김정현, 민관식, 백혜자, Dae-Geun Kim한국수산과학회 · Fisheries and Aquatic Sciences2022 · 제25권 제1호Specific Biological Activity of Equine Chorionic Gonadotropin (eCG) Glycosylation Sites in Cells Expressing Equine Luteinizing Hormone/CG (eLH/ CG) Receptor민관식, Gyeong-Eun Park, Hyo-Eun Joo, Munkhzaya Byambaragchaa, Myung-Hwa Kang, Sang-Gwon Kim, Seung-Hee Choi, Yean-Ji Kim한국발생생물학회 · 발생과 생식2021 · 제25권 제4호

이 간행물의 다른 논문 (11건)