열목어 재조합 생식선자극호르몬(mt-rGTH)에 의한 암컷 뱀장어의 성성숙 유도
Maturation Induction by Manchurian Trout Recombinant Gonadotropin Hormone(mt-rGTH) in Female Eel, Anguilla japonica
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In the present study, we investigated in vivo effects of Manchurian trout recombinant gonadotrophin hormone (r-mtGTH) on the induction of maturation in female eel, Anguilla japonica. The brood stock, female eel (450±50 g) were weekly injected intramuscularly with different doses of 0.1, 1, 10 μg/mL/fish (r-mtFSH or r-mtLH) for 10 week. The effects of r-mtGTH were analyzed by gonadosomatic index (GSI), ovarian follicle diameter and sex steroid levels. All groups did not exhibit significant differences in the GSI values. Whereas plasma testosterone (T) and estradiol-17β (E2) levels did not change significantly in control group, plasma levels of T and E2 by injection of the r-mtFSH or r-mtLH were increased at 2 or 4 week after injection. In addition, injection of the r-mtFSH (1, 10 μg/mL/fish) or r-mtLH (0.1, 1, 10 μg/mL/fish) significantly increased follicle diameters comparing to the control group. These results demonstrate that the recombinant hormone may affect early ovary development and maturation in female eel. Taken together, these results suggest that effect of the recombinant FSH and LH should be further studied in female eel.
Abstract
In the present study, we investigated in vivo effects of Manchurian trout recombinant gonadotrophin hormone (r-mtGTH) on the induction of maturation in female eel, Anguilla japonica. The brood stock, female eel (450±50 g) were weekly injected intramuscularly with different doses of 0.1, 1, 10 μg/mL/fish (r-mtFSH or r-mtLH) for 10 week. The effects of r-mtGTH were analyzed by gonadosomatic index (GSI), ovarian follicle diameter and sex steroid levels. All groups did not exhibit significant differences in the GSI values. Whereas plasma testosterone (T) and estradiol-17β (E2) levels did not change significantly in control group, plasma levels of T and E2 by injection of the r-mtFSH or r-mtLH were increased at 2 or 4 week after injection. In addition, injection of the r-mtFSH (1, 10 μg/mL/fish) or r-mtLH (0.1, 1, 10 μg/mL/fish) significantly increased follicle diameters comparing to the control group. These results demonstrate that the recombinant hormone may affect early ovary development and maturation in female eel. Taken together, these results suggest that effect of the recombinant FSH and LH should be further studied in female eel.
참고문헌 (18)
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