택사와 alisol B acetate의 병용 투여가 천식 동물 모델에 미치는 영향
Anti-asthmatic Effect of Alismatis Rhizoma and Alisol Acetate B Combination Therapy in a Murine Asthma Model
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objectives: The aim of the study was to evaluate the anti-asthmatic effect of alismatis rhizoma and alisol acetate B combination therapy in a murine asthma model. Methods: C57BL/6 mice were sensitized to and challenged with a mixture of ragweed, dust mite, and aspergillus to induce an asthma animal model. Alismatis rhizoma extract and alisol acetate B combination therapy was co-administered only in the experimental group. To evaluate the anti-asthmatic effect of the combination therapy, inflammatory cell counts in bronchoalveolar lavage (BAL) fluid were determined, and tissue was examined histologically with hematoxylin and eosin (H & E) and periodic acid-Schiff (PAS) stains, by enzyme-linked immunosorbent assay (ELISA) of IgE, IL-4, and IL-5, and with reverse transcription polymerase chain reaction (RT-PCR) of IL-5, IL-33, MUC5AC. Results: Alismatis rhizoma and alisol acetate B combination therapy reduced the number of inflammatory cells, alleviated histologic features, and down-regulated all the investigated asthma mediators, IgE, IL-4, IL-5, IL-33, and MUC5AC. Conclusions: According to the above results, alismatis rhizoma and alisol acetate B combination therapy may have therapeutic potential for asthma.
Abstract
Objectives: The aim of the study was to evaluate the anti-asthmatic effect of alismatis rhizoma and alisol acetate B combination therapy in a murine asthma model. Methods: C57BL/6 mice were sensitized to and challenged with a mixture of ragweed, dust mite, and aspergillus to induce an asthma animal model. Alismatis rhizoma extract and alisol acetate B combination therapy was co-administered only in the experimental group. To evaluate the anti-asthmatic effect of the combination therapy, inflammatory cell counts in bronchoalveolar lavage (BAL) fluid were determined, and tissue was examined histologically with hematoxylin and eosin (H & E) and periodic acid-Schiff (PAS) stains, by enzyme-linked immunosorbent assay (ELISA) of IgE, IL-4, and IL-5, and with reverse transcription polymerase chain reaction (RT-PCR) of IL-5, IL-33, MUC5AC. Results: Alismatis rhizoma and alisol acetate B combination therapy reduced the number of inflammatory cells, alleviated histologic features, and down-regulated all the investigated asthma mediators, IgE, IL-4, IL-5, IL-33, and MUC5AC. Conclusions: According to the above results, alismatis rhizoma and alisol acetate B combination therapy may have therapeutic potential for asthma.
참고문헌 (35)
- James AL, "Airway smooth muscle thickness in asthma is related to severity but not duration of asthma", Eur Respir J, 34(5), pp.1040~1045 (2009)
- Anandan C, "Is the prevalence of asthma declining? Systematic review of epidemiological studies", Allergy, 65(2), pp.152~167 (2010)
- GBD 2015 Disease and Injury Incidence and Prevalence Collaborators, "Global, regional, and national incidence, prevalence, and years lived with disability for 310 diseases and injuries, 1990-2015: a systematic analysis for the Global Burden of Disease Study 2015", Lancet, 388(10053), pp.1545~1602 (2016)
- GBD 2015 Mortality and Causes of Death Collaborators, "Global, regional, and national life expectancy, all-cause mortality, and cause-specific mortality for 249 causes of death, 1980-2015: a systematic analysis for the Global Burden of Disease Study 2015", Lancet, 388(10053), pp.1459~1544 (2016)
- "Korea National Health and Nutrition Examination Survey (KNHANES VI-3)" (2015)[URL]
- McCracken JL, "Diagnosis and management of asthma in adults: a review", JAMA, 318(3), pp.279~290 (2017)
- Donnelly LE, "Novel targets and drugs in inflammatory lung disease", Curr Opin Pharmacol, 8(3), pp.219~221 (2008)
- Thomson NC, "New and developing non-adrenoreceptor small molecule drugs for the treatment of asthma", Expert Opin Pharmacother, 18(3), pp.283~293 (2017)
- Wu JN, "An illustrated Chinese materia medica", pp.706~ (2005), Oxford University Press
- Hong-Guang Jin, "A New Triterpenoid from Alisma orientale and Their Antibacterial Effect", Archives of Pharmacal Research, 35(11), pp.1919~1926 (2012), 대한약학회
- Han CW, "Antioxidative effects of Alisma orientale extract in palmitate-induced cellular injury", Pharm Biol, 50(10), pp.1281~1288 (2012)
- Dai Y, "Anti-inflammatory activities and effect of rhizoma Alismatis on immune system", Zhongguo Zhong Yao Za Zhi, 16(10), pp.622~625 (1991)
- Kubo M, "Studies on Alismatis rhizoma. I. Anti-allergic effects of methanol extract and six terpene components from Alismatis rhizoma (dried rhizome of Alisma orientale)", Biol Pharm Bull, 20(5), pp.511~516 (1997)
- Kim KH, "Therapeutic Effect of the Tuber of Alisma orientale on Lipopolysaccharide-Induced Acute Lung Injury", Evid Based Complement Alternat Med, 2013, pp.863892~ (2013)
- 박종철, "택사에서 Alisol B 23-Acetate의 분리 및 함량분석", 한국식품영양과학회지, 34(2), pp.243~246 (2005), 한국식품영양과학회
- Lee JH, "The rhizomes of Alisma orientale and alisol derivatives inhibit allergic response and experimental atopic dermatitis", Biol Pharm Bull, 35(9), pp.1581~1587 (2012)
- Kanno Y, "Alisol B 23-acetate from the rhizomes of Alisma orientale is a natural agonist of the human pregnane X receptor", Phytomedicine, 26, pp.22~27 (2017)
- Wenzel SE, "Asthma: defining of the persistent adult phenotypes", Lancet, 368(9537), pp.804~813 (2006)
- Hesselmar B, "The heterogeneity of asthma phenotypes in children and young adults", J Allergy (Cairo), 2012, pp.163089~ (2012)
- Simpson JL, "Differential proteolytic enzyme activity in eosinophilic and neutrophilic asthma", Am J Respir Crit Care Med, 172(5), pp.559~565 (2005)
- Sagar S, "Translational value of animal models of asthma: Challenges and promises", Eur J Pharmacol, 759, pp.272~277 (2015)
- Kianmeher M, "Animal model of asthma, various methods and measured parameters: a methodological review", Iran J Allergy Asthma Immunol, 15(6), pp.445~465 (2016)
- Zosky G, "Animal models of asthma", Clin Exp Allergy, 37(7), pp.973~988 (2007)
- Fuchs B, "Improved mouse models of allergy and allergic asthma-chances beyond ovalbumin", Curr Drug Targets, 9(6), pp.495~502 (2008)
- Goplen N, "Combined sensitization of mice to extracts of dust mite, ragweed, and Aspergillus species breaks through tolerance and establishes chronic features of asthma", J Allergy Clin Immunol, 123(4), pp.925~932 (2009)
- Van Hove CL, "Prolonged inhaled allergen exposure can induce persistent tolerance", Am J Respir Cell Mol Biol, 36(5), pp.573~584 (2007)
- Nemeth K, "Bone marrow stromal cells use TGF-beta to suppress allergic responses in a mouse model of ragweed -induced asthma", Proc Natl Acad Sci USA, 107(12), pp.5652~5657 (2010)
- Wan YY, "GATA3: a master of many trades in immune regulation", Trends Immunol, 35(6), pp.233~242 (2014)
- Novak N, "IgE receptors", Curr Opin Immunol, 13(6), pp.721~726 (2001)
- Webb DC, "Comparative roles of IL-4, IL-13, and IL-4Ralpha in dendritic cell maturation and CD4+ Th2cell function", J Immunol, 178(1), pp.219~227 (2007)
- Leckie MJ, "Anti-interleukin-5 monoclonal antibodies:preclinical and clinical evidence in asthma models", Am J Respir Med, 2(3), pp.245~259 (2003)
- Drake LY, "IL-33: biological properties, functions, and roles in airway disease", Immunol Rev, 278(1), pp.173~184 (2017)
- Evans CM, "The polymeric mucin Muc5ac is required for allergic airway hyperreactivity", Nat Commun, 6, pp.6281~ (2015)