Cryopreservation of Semen in Dead Yellow Croaker, Larimichthys polyactis
0
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
This study demonstrated that cryopreserved semen from dead fish can be used for seedling production. Yellow croakers, Larimichthys polyactis, were killed and stored at temperatures of 20°C or 0°C for 6 hours. At 2 hour intervals, semen from these fish was collected using abdominal pressure and evaluated for spermatozoa motility and semen cryopreservation. Semen collected after 6 hours from dead fish stored at 0°C could be cryopreserved and attained fertilization and hatching rates of 15.0±1.2% and 14.8±1.6%, respectively. This study suggests that germ cells such as the semen of dead fish can be cryopreserved and utilized in the restoration of a species.
Abstract
This study demonstrated that cryopreserved semen from dead fish can be used for seedling production. Yellow croakers, Larimichthys polyactis, were killed and stored at temperatures of 20°C or 0°C for 6 hours. At 2 hour intervals, semen from these fish was collected using abdominal pressure and evaluated for spermatozoa motility and semen cryopreservation. Semen collected after 6 hours from dead fish stored at 0°C could be cryopreserved and attained fertilization and hatching rates of 15.0±1.2% and 14.8±1.6%, respectively. This study suggests that germ cells such as the semen of dead fish can be cryopreserved and utilized in the restoration of a species.
참고문헌 (18)
- Babiak I, "Cryopreservation of sperm of common carp Cyprinus carpio", Aquaculture Research, 28, pp.567~571 (1997)
- Bencic DC, "Carbon dioxide reversibly inhibits sperm motility and fertilizing ability in steelhead Oncorhynchus mykiss", Fish Physiol Biochem, 23, pp.275~281 (2000)
- Billard R, "Survie in vitro et post mortem des gametes de truite fario Salmo gairdneri", Can J Zool, 59, pp.29~33 (1981)
- Cabrita E, "Effect of different cryoprotectants and vitrificant solutions on the hatching rate of turbot Scophthalmus maximus embryos", Cryobiology, 47, pp.204~213 (2003)
- Chao NH, "Cryopreservation of finfish and shellfish gametes and embryos", Aquaculture, 197, pp.161~189 (2001)
- Dietrich GJ, "Motility parameters of rainbow trout (Oncorhynchus mykiss) spermatozoa in relation to sequential collection of milt, time of post-mortem storage and anesthesia", Fish Physiol Biochem, 31, pp.1~9 (2005)
- Fujinami Y, "Sperm motility and short-term preservation of testicular spermatozoa obtained from captured and dead red tilefish Branchiostegus japonicas", Nippon Suisan Gakkaishi, 69, pp.162~169 (2003)
- Gwo JC, "Cryopreservation of sperm of some marine fishes. In: Cryopreservation in aquatic species", pp.138~160 (2000), World Aquaculture Society
- Horváth Á, "Cryopreservation of common carp sperm", Aquat Living Resour, 16, pp.457~460 (2003)
- Kim SA, "The effect of seasonal anomalies of seawater temperature and salinity on the fluctuation in yields of small yellow croaker, Larimichthys polyactis, in the Yellow Sea", Fisheries Oceanography, 6, pp.1~9 (1997)
- Koteeswaran R, "Live sperm from post-mortem preserved Indian catfish", Current Science, 82, pp.447~450 (2002)
- Lahnsteiner F, "Semen cryopreservation in the Salmonidae and in the Northern pike", Aquaculture Research, 31, pp.245~258 (2000)
- Le MH, "Yellow croaker Larimichthys polyactis (Teleostei) semen properties", Proceedings of the meeting, 2009 of the Korean Society of Developmental Biology, pp.76~76 (2009)
- 레민황, "Diluents and cryoprotectants for cryopreservation of Filefish Thamnaconus modestus sperm", 한국양식학회지, 21(1), pp.54~59 (2008), 한국양식학회
- 임한규, "강도다리(Platichthys stellatus) 정자의 냉동보존에 미치는 희석액과 동해방지제의 영향", 한국양식학회지, 20(3), pp.173~177 (2007), 한국양식학회
- Lim HK, "Effects of diluents and cryo-protectants on sperm cryopreservation of masou salmon Oncorhynchus masou masou", J Kor Fish Soc, 41, pp.267~271 (2008)
- Routray P, "Cryopreservation of dead fish spermatozoa several hours after death of Indian major carp, Labeo rohita and its successful utilization in fish production", Aquaculture, 261, pp.1204~1211 (2006)
- Suquet M, "Cryopreservation of sperm in marine fish", Aquaculture Research, 31, pp.231~243 (2000)