연육 제조과정에서 발생하는 어피 부산물의 활용을 위한 Cosmeceutical 활성 평가
Evaluation of Cosmeceutical Properties of Fish Skin By-product Hydrolysates Collected During Surimi Manufacturing Process
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초록
This study investigated the cosmetic effects of enzymatic hydrolytes of an aquatic by-product, fish skin. The skins ofolive flounder Paralichthys olivaceus (PO) and Alaska pollock Gadus chalcogrammus (AP) were hydrolyzed usingpepsin, Alcalase, and Protemax. Three enzymatic hydrolytes were obtained and the inhibitory effects of these hydrolyteson the aging-related enzymes tyrosinase, elastase, and collagenase were determined. The results indicated thatthe pepsin hydrolytes of PO and PA had stronger activities than the other hydrolytes. PO and PA also significantlyreduced the intracellular reactive oxygen species levels in and improved the viability of H2O2-treated Vero cells;decreased nitric oxide production by and increased the cell viability of lipopolysaccharide-treated RAW 264.7 cells;and reduced intracellular reactive oxygen species levels and improved the viability of ultraviolet B irradiated HaCaTcells and human dermal fibroblasts. Furthermore, PO and PA remarkably reduced the intra- and extracellular melanincontents of alpha-melanocyte-stimulating hormone-stimulated B16F10 cells. These results demonstrate that PO andPA have potential for use in the cosmetic industry.
Abstract
This study investigated the cosmetic effects of enzymatic hydrolytes of an aquatic by-product, fish skin. The skins ofolive flounder Paralichthys olivaceus (PO) and Alaska pollock Gadus chalcogrammus (AP) were hydrolyzed usingpepsin, Alcalase, and Protemax. Three enzymatic hydrolytes were obtained and the inhibitory effects of these hydrolyteson the aging-related enzymes tyrosinase, elastase, and collagenase were determined. The results indicated thatthe pepsin hydrolytes of PO and PA had stronger activities than the other hydrolytes. PO and PA also significantlyreduced the intracellular reactive oxygen species levels in and improved the viability of H2O2-treated Vero cells;decreased nitric oxide production by and increased the cell viability of lipopolysaccharide-treated RAW 264.7 cells;and reduced intracellular reactive oxygen species levels and improved the viability of ultraviolet B irradiated HaCaTcells and human dermal fibroblasts. Furthermore, PO and PA remarkably reduced the intra- and extracellular melanincontents of alpha-melanocyte-stimulating hormone-stimulated B16F10 cells. These results demonstrate that PO andPA have potential for use in the cosmetic industry.
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