2019년 서해 연안 패류 및 양식 새우(Litopenaeus vannamei)에서 분리한 Vibrio parahaemolyticus 균주들과 새우 급성간췌장괴사병(AHPND)과의 연관성
The Relationship Between Acute Hepatopancreatic Necrosis Disease (AHPND) in Shrimp Litopenaeus vannamei and Vibrio parahaemolyticus Strains Isolated from Shellfish and Shrimp of the West Coast of Korea in 2019
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Acute hepatopancreatic necrosis disease (AHPND), previously known as early mortality syndrome (EMS), is an emerging disease in shrimp caused by Vibrio parahaemolyticus. Some V. parahaemolyticus strains are associated with foodborne diseases in humans. To date, studies on the relationship between AHPND and pathogenic V. parahaemolyticus are very limited. In this study, we monitored the thermostable direct hemolysin-related hemolysin (trh) gene and AHPND–related genes, such as Photorhabdus insect-related (pir) genes, in 892 strains of V. parahaemolyticus isolated and identified in 24 areas of the West Coast of Korea from May to October 2019. The trh gene was detected in 9.6% of the isolates from short neck clam samples. However, the pirA and pirB genes related to AHPND were not found in any of the isolates despite using both duplex and nested PCR assays, suggesting that AHPND-related genes were nonexistent in the V. parahaemolyticus strains isolated. This study contributes to the current understanding of the relationship between AHPND and V. parahaemolyticus in Korea, as well as provides data on spatial and seasonal distributions of V. parahaemolyticus.
Abstract
Acute hepatopancreatic necrosis disease (AHPND), previously known as early mortality syndrome (EMS), is an emerging disease in shrimp caused by Vibrio parahaemolyticus. Some V. parahaemolyticus strains are associated with foodborne diseases in humans. To date, studies on the relationship between AHPND and pathogenic V. parahaemolyticus are very limited. In this study, we monitored the thermostable direct hemolysin-related hemolysin (trh) gene and AHPND–related genes, such as Photorhabdus insect-related (pir) genes, in 892 strains of V. parahaemolyticus isolated and identified in 24 areas of the West Coast of Korea from May to October 2019. The trh gene was detected in 9.6% of the isolates from short neck clam samples. However, the pirA and pirB genes related to AHPND were not found in any of the isolates despite using both duplex and nested PCR assays, suggesting that AHPND-related genes were nonexistent in the V. parahaemolyticus strains isolated. This study contributes to the current understanding of the relationship between AHPND and V. parahaemolyticus in Korea, as well as provides data on spatial and seasonal distributions of V. parahaemolyticus.
참고문헌 (41)
- "Vibrio species causing vibriosis" (2019)[URL]
- Sirintip Dangtip, "AP4 method for two-tube nested PCR detection of AHPND isolates of Vibrio parahaemolyticus", Aquaculture Reports, 2, pp.158~162 (2015), Elsevier BV[DOI]
- AK Dhar, "First report of acute hepatopancreatic necrosis disease (AHPND) occurring in the USA", Diseases of Aquatic Organisms, 132(3), pp.241~247 (2019), Inter-Research Science Center[DOI]
- Elliot EL, "Bacteriological analytical manual", pp.9.01~9.27 (1995), FDA
- Farmer JJ III, "Bergey’s manual of systematics of archaea and bacteria" (2015), John Wiley Sons
- Bo Feng, "Diversity analysis of acute hepatopancreatic necrosis disease-positive Vibrio parahaemolyticus strains", Aquaculture and Fisheries, 2(6), pp.278~285 (2017), Elsevier BV[DOI]
- Songzhe Fu, "Delineating the Origins of Vibrio parahaemolyticus Isolated from Outbreaks of Acute Hepatopancreatic Necrosis Disease in Asia by the Use of Whole Genome Sequencing", Frontiers in Microbiology, 8, pp.2354~ (2017), Frontiers Media SA[DOI]
- JE Han, "Photorhabdus insect-related (Pir) toxin-like genes in a plasmid of Vibrio parahaemolyticus, the causative agent of acute hepatopancreatic necrosis disease (AHPND) of shrimp", Diseases of Aquatic Organisms, 113(1), pp.33~40 (2015), Inter-Research Science Center[DOI]
- Honda T, "The pathogenicity of Vibrio parahaemolyticus and the role of the thermostable direct haemolysin and related haemolysins", Rev Med Microbiol, 4, pp.106~113 (1993)
- Hwang SD, "Whole genome sequence of acute hepatopancreatic necrosis (AHPND) isolated from Korea", Proceedings of the Korean Federation of Fisheries Science and Technology Societies (KOFFST) International Conference 2018, pp.492~ (2018)
- Jyoti Joshi, "Variation in Vibrio parahaemolyticus isolates from a single Thai shrimp farm experiencing an outbreak of acute hepatopancreatic necrosis disease (AHPND)", Aquaculture, 428-429, pp.297~302 (2014), Elsevier BV[DOI]
- Chang-Ho Kang, "Characterization of Vibrio parahaemolyticus isolated from oysters in Korea: Resistance to various antibiotics and prevalence of virulence genes", Marine Pollution Bulletin, 118(1-2), pp.261~266 (2017), Elsevier BV[DOI]
- Chang-Ho Kang, "Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from oysters in Korea", Environmental Science and Pollution Research, 23(1), pp.918~926 (2016), Springer Science and Business Media LLC[DOI]
- Nam-Eun Kim, "Acute hepatopancreatic necrosis disease of shrimp and import health measures", Journal of fish pathology, 28(1), pp.1~7 (2015), The Korean Society of Fish Pathology[DOI]
- 김수경, "양식 굴(Crassostrea gigas)에서 분리된 장염비브리오균의독소 유전자 보유 및 항균제 감수성", 한국수산과학회지, 49(2), pp.116~123 (2016), 한국수산과학회
- Kondo H, "Draft genome sequences of six strains of Vibrio parahaemolyticus isolated from early mortality syndrome", acute hepatopancreatic necrosis disease shrimp in Thailand, 2014, pp.2, e00221~14
- J Kongrueng, "Characterization of Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease in southern Thailand", Journal of Fish Diseases, 38(11), pp.957~966 (2015), Wiley[DOI]
- Chung-Te Lee, "The opportunistic marine pathogen Vibrio parahaemolyticus becomes virulent by acquiring a plasmid that expresses a deadly toxin", Proceedings of the National Academy of Sciences, 112(34), pp.10798~10803 (2015), Proceedings of the National Academy of Sciences[DOI]
- 이동수, "곰소만의 해수 및 패류에 있어서 장염비브리오균(Vibrio parahaemolyticus)과 비브리오패혈증균(Vibrio vulnificus)의 분포", 한국수산과학회지, 52(2), pp.114~120 (2019), 한국수산과학회[DOI]
- Jong-Kyung Lee, "Occurrence of Vibrio parahaemolyticus in oysters from Korean retail outlets", Food Control, 19(10), pp.990~994 (2008), Elsevier BV[DOI]
- "Early mortality syndrome affects shrimp in Asia" (2012)[URL]
- Tan Turk Hsern Malcolm, "Detection and quantification of pathogenic Vibrio parahaemolyticus in shellfish by using multiplex PCR and loop-mediated isothermal amplification assay", Food Control, 47, pp.664~671 (2015), Elsevier BV[DOI]
- Thangapandi Marudhupandi, "Vibrio parahaemolyticus a causative bacterium for tail rot disease in ornamental fish, Amphiprion sebae", Aquaculture Reports, 8, pp.39~44 (2017), Elsevier BV[DOI]
- Standard manual of whiteleg shrimp farming, "NIFS", NIFS, ~ (2018)
- 박큰바위, "2013-2016년 경남 연안 해수 및 패류에서 병원성 비브리오균의 계절적 및 지역적 변동", 한국수산과학회지, 52(1), pp.27~34 (2019), 한국수산과학회[DOI]
- Kunbawui Park, "Occurrence and virulence of Vibrio parahaemolyticus isolated from seawater and bivalve shellfish of the Gyeongnam coast, Korea, in 2004–2016", Marine Pollution Bulletin, 137, pp.382~387 (2018), Elsevier BV[DOI]
- 박용수, "남해안 패류양식장에서 분리한 Vibrio parahaemolyticus의 병원인자분포 및 항균제내성", 한국수산과학회지, 49(4), pp.460~466 (2016), 한국수산과학회
- Aidan C. Parte, "LPSN – List of Prokaryotic names with Standing in Nomenclature (bacterio.net), 20 years on", International Journal of Systematic and Evolutionary Microbiology, 68(6), pp.1825~1829 (2018), Microbiology Society[DOI]
- Xinjie Song, "Occurrence and Identification of Pathogenic Vibrio Contaminants in Common Seafood Available in a Chinese Traditional Market in Qingdao, Shandong Province", Frontiers in Microbiology, 11, pp.1488~ (2020), Frontiers Media SA[DOI]
- P.S Sudheesh, "Pathogenicity of Vibrio parahaemolyticus in tiger prawn Penaeus monodon Fabricius: possible role of extracellular proteases", Aquaculture, 196(1-2), pp.37~46 (2001), Elsevier BV[DOI]
- Thompson CA, "Serological and hemolytic characteristics of Vibrio parahaemolyticus from marine sources", J Food Sci, 41, pp.204~205 (1976)
- Thompson FL, "The biology of vibrios", pp.190~203 (2006), ASM Press
- L Tran, "Determination of the infectious nature of the agent of acute hepatopancreatic necrosis syndrome affecting penaeid shrimp", Diseases of Aquatic Organisms, 105(1), pp.45~55 (2013), Inter-Research Science Center[DOI]
- Hidetoshi Urakawa, "Characterization of depth-related population variation in microbial communities of a coastal marine sediment using 16S rDNA-based approaches and quinone profiling", Environmental Microbiology, 2(5), pp.542~554 (2000), Wiley[DOI]
- Akio Yamamoto, "Quantitative modeling for risk assessment of Vibrio parahaemolyticus in bloody clams in southern Thailand", International Journal of Food Microbiology, 124(1), pp.70~78 (2008), Elsevier BV[DOI]
- Ji Hye Yang, "Distribution and antimicrobial susceptibility of Vibrio species associated with zooplankton in coastal area of Korea", Marine Pollution Bulletin, 125(1-2), pp.39~44 (2017), Elsevier BV[DOI]
- Qian Yang, "Comparative genomic analysis unravels the transmission pattern and intra-species divergence of acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio parahaemolyticus strains", Molecular Genetics and Genomics, 294(4), pp.1007~1022 (2019), Springer Science and Business Media LLC[DOI]
- Y.-T. Yang, "Draft Genome Sequences of Four Strains of Vibrio parahaemolyticus, Three of Which Cause Early Mortality Syndrome", Acute Hepatopancreatic Necrosis Disease in Shrimp in China and Thailand, 2014, pp.2(5), e00816-14~[DOI]
- 유홍식, "한국 연안산 패류 중 Vibrio parahaemolyticus의 분포 및 항생제내성 특성 비교", 한국수산과학회지, 47(5), pp.508~515 (2014), 한국수산과학회
- Zhang BC, "Isolation, identification, and pathogenicity analysis of a Vibrio parahaemolyticus strain from Litopenaeus vannamei", Prog Fish Sci, 33, pp.56~62 (2012)[DOI]
- Zorriehzahra MJ, "Early mortality syndrome(EMS)as new emerging threat in shrimp industry", Adv Anim Vet Sci, 3, pp.64~72 (2015)[DOI]
