木瓜추출물이 급성 간손상 흰쥐에 미치는 효과
The Effect of Chaenomelis Fructus Extract on Acute Hepatic Injury in Rats
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Objective: This study was conducted to investigate the effect of Chaenomelis Fructus (CF) water extract on thioacetamide (TAA)-treated rats. Methods: Rats were divided into five groups: one normal group (n=8) and four with TAA-induced hepatic injury. These treatment groups were administered distilled water (n=8); silymarin 100 mg/kg (n=8); CF 100 mg/kg (n=8); and CF 200 mg/kg (n=8). In the TAA groups, the acute liver injury was induced via IP injection (200 mg/kg), and the silymarin and CF extract were then orally administered for three days. Subsequently, serum levels of GOT, GPT, and ammonia were confirmed as well as protein expressions using liver tissue. Results: In the liver injury-induced rats, CF administration reduced tissue damage and serum levels of GOT, GPT, and ammonia. In addition, CF increased the anti-oxidant proteins Nrf2, Keap1, HO-1, and catalase and significantly regulated matrix metalloproteinases (MMP-2 and MMP-9) and their tissue inhibitors (TIMP-1 and TIMP-2). Conclusions: In this animal model of liver injury induced by TAA, CF extract is determined to have a hepatoprotective effect by increasing anti-oxidant proteins that relieve damage and by regulating the expression of matrix metalloproteinases.
Abstract
Objective: This study was conducted to investigate the effect of Chaenomelis Fructus (CF) water extract on thioacetamide (TAA)-treated rats. Methods: Rats were divided into five groups: one normal group (n=8) and four with TAA-induced hepatic injury. These treatment groups were administered distilled water (n=8); silymarin 100 mg/kg (n=8); CF 100 mg/kg (n=8); and CF 200 mg/kg (n=8). In the TAA groups, the acute liver injury was induced via IP injection (200 mg/kg), and the silymarin and CF extract were then orally administered for three days. Subsequently, serum levels of GOT, GPT, and ammonia were confirmed as well as protein expressions using liver tissue. Results: In the liver injury-induced rats, CF administration reduced tissue damage and serum levels of GOT, GPT, and ammonia. In addition, CF increased the anti-oxidant proteins Nrf2, Keap1, HO-1, and catalase and significantly regulated matrix metalloproteinases (MMP-2 and MMP-9) and their tissue inhibitors (TIMP-1 and TIMP-2). Conclusions: In this animal model of liver injury induced by TAA, CF extract is determined to have a hepatoprotective effect by increasing anti-oxidant proteins that relieve damage and by regulating the expression of matrix metalloproteinases.
참고문헌 (24)
- 全國韓醫科大學 肝系內科學 교수, "肝系內 科學", pp.164~176 (1992), 東洋醫科學硏究院
- Campos R, "Silybin dihemisuccinate protects against glutathione depletion and lipid peroxidation indeced by acetaminophen on rat liver", Planta Medica, 55(5), pp.417~419 (1989)
- Junqueira VB, "Dose-dependent study of the effects of acute lindane administration on rat liver superoxide anion production, antioxidant enzyme activities and lipid peroxidation", Toxicology, 41(2), pp.193~204 (1986)
- Moody CS, "Mutagenicity of oxygen free radicals", Proc Natl Acad Sci USA, 79(9), pp.2855~2859 (1982)
- Murakami T, "Hepatitis, cirrhosis, and hepatoma", J Magn Reson Imaging, 8(2), pp.346~358 (1998)
- Reichen J, "Assessment of hepatic function with xenobiotics", Semin Liver Dis, 15(3), pp.189~201 (1995)
- Aratani Y, "Myeloperoxidase:Its role for host defense, inflammation, and neutrophil function", Arch Biochem Biophys, 640, pp.47~52 (2018)
- 류한우, "모과(木瓜) 물추출물의 항염증 효능에 관한 실험적 연구", 대한한방부인과학회지, 25(3), pp.1~15 (2012), 대한한방부인과학회[DOI]
- 김주은, "모과 에탄올 추출물의 아세틸콜린에스테라제 저해활성과 신경세포에서 아밀로이드 전구단백질의 대사에 미치는 영향", 생약학회지, 46(4), pp.327~333 (2015), 한국생약학회
- 강석용, "모과추출물의 C2C12 근육세포에서 근분화 및 에너지대사조절인자 발현 증진 효과 연구", 대한본초학회지, 34(6), pp.99~107 (2019), 대한본초학회[DOI]
- 김용민, "구기자 복합물 약침액이 간기능 개선에 미치는 영향", Korean Journal of Acupuncture, 36(4), pp.221~229 (2019), 경락경혈학회[DOI]
- 김진배, "급성 독성 간손상의 임상적 양상", Clinical and Molecular Hepatology, 10(2004), pp.125~134 (2004), 대한간학회
- 유지현, "모과 추출물의 항응혈 활성", 대한본초학회지, 24(2), pp.7~11 (2009), 대한본초학회
- 이명렬, "모과 에탄올 추출물의 항산화 효과", Food Science and Preservation, 14(2), pp.177~182 (2007), 한국식품저장유통학회
- 김다혜, "3T3L-1 지방전구세포와 고지방식이로 유도된 비만 마우스 모델에서 모과 추출물의 항비만 효과와 억제 기전", 대한한의학 방제학회지, 25(4), pp.457~469 (2017), 대한한의학방제학회[DOI]
- 이유미, "에탄올에 의해 유발된 간독성에 대한 모과 추출물의 보호효과", 한국식품영양과학회지, 35(10), pp.1336~1342 (2006), 한국식품영양과학회
- 이대형, "모과(Chaenomeles sinensis)주류의 생리기능성", KSBB Journal, 17(3), pp.266~270 (2002), 한국생물공학회
- Fitzhugh OG, "Liver Tumors in Rats Fed Thiourea or Thioacetamide", Science, 108(2814), pp.626~628 (1948)
- Kim SY, "The effect of betaine on the CC14-induced hepatoxicity in rats", Yakhak hoeji, 37(5), pp.538~543 (1993)
- Germoush MO, "Umbelliferone prevents oxidative stress, inflammation and hematological alterations, and modulates glutamate-nitric oxide-cGMP signaling in hyperammonemic rats", Biomed Pharmacother, 102, pp.392~402 (2018)
- 김경조, "Thioacetamide 유발 간손상모델에서 통규활혈탕의 간보호효과", 대한본초학회지, 33(1), pp.37~46 (2018), 대한본초학회[DOI]
- He HJ, "Curcumin attenuates Nrf2 signaling defect, oxidative stress in muscle and glucose intolerance in high fat diet-fed mice", World J Diabetes, 3(5), pp.94~104 (2012)
- Lee MS, "Ondamtanggamibang protects neurons from oxidative stress with induction of heme oxygenase-1", J Ethnopharmacol, 108(2), pp.294~298 (2016)
- 김성은, "궤양성 대장염에서 기질단백분해효소(Matrix Metallopreoteinases)와 억제인자(Tissue Inhibitor of Metalloproteinase)의 mRNA 발현", Intestinal research, 7(1), pp.32~40 (2009), 대한장연구학회