금은화가 HUVEC에서 죽상동맥경화증 관련 염증인자 발현 억제에 미치는 영향
The Effect of Lonicera Japonica Thunberg on Inflammatory Factor Expression Associated with Atherosclerosis
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Objective: The purpose of this study was to investigate the effect of Lonicera Japonica Thunberg (LJT) on the inflammatory factor expression associated with atherosclerosis in human umbilical vein endothelial cells (HUVECs). Methods: After treatment with LJT in HUVEC which is treated with TNF-α, we measured the expression levels of biomarkers (MCP-1, ICAM-1, VCAM-1, KLF2, and eNOS), mRNA (CCL2, ICAM1, VCAM1, KLF2, and NOS3), and the proteins (MCP-1, ICAM-1, VCAM-1, KLF2, eNOS, ERK, JNK, and p38). Results: 1. Compared to the control, LJT significantly reduced MCP-1 and VCAM-1 levels at concentrations of 100, 200, and 400 µg/ml and ICAM-1 expression at 200 and 400 µg/ml compared to the control. It increased KLF2 levels at all three concentrations, but not significantly, while eNOS expression was significantly increased at 400 µg/ml. 2. LJT was seen to significantly reduce the expression of CCL2, ICAM1, and VCAM1 mRNA at concentrations of 100, 200, and 400 µg/ml compared to the control. In contrast, significantly increased KLF2 and NOS3 mRNA levels were observed at 400 µg/ml and at 200 and 400 µg/ml, respectively. 3. Compared to the control, LJT significantly reduced the protein expression of MCP-1 and VCAM-1 at 200 and 400 µg/ml and of ICAM-1 at 400 µg/ml. In addition, it increased both KLF2 and eNOS protein levels at 200 and 400 µg/ml. Although LJT did not have an effect on ERK expression in comparison with the control, it significantly reduced JNK levels at 200 and 400 µg/ml and p38 levels at 400 µg/ml. Conclusions: These results suggest that LJT has an effect on the inhibition of inflammatory factor expression associated with atherosclerosis in HUVECs which could contribute to the prevention of cardiovascular and cerebrovascular diseases.
Abstract
Objective: The purpose of this study was to investigate the effect of Lonicera Japonica Thunberg (LJT) on the inflammatory factor expression associated with atherosclerosis in human umbilical vein endothelial cells (HUVECs). Methods: After treatment with LJT in HUVEC which is treated with TNF-α, we measured the expression levels of biomarkers (MCP-1, ICAM-1, VCAM-1, KLF2, and eNOS), mRNA (CCL2, ICAM1, VCAM1, KLF2, and NOS3), and the proteins (MCP-1, ICAM-1, VCAM-1, KLF2, eNOS, ERK, JNK, and p38). Results: 1. Compared to the control, LJT significantly reduced MCP-1 and VCAM-1 levels at concentrations of 100, 200, and 400 µg/ml and ICAM-1 expression at 200 and 400 µg/ml compared to the control. It increased KLF2 levels at all three concentrations, but not significantly, while eNOS expression was significantly increased at 400 µg/ml. 2. LJT was seen to significantly reduce the expression of CCL2, ICAM1, and VCAM1 mRNA at concentrations of 100, 200, and 400 µg/ml compared to the control. In contrast, significantly increased KLF2 and NOS3 mRNA levels were observed at 400 µg/ml and at 200 and 400 µg/ml, respectively. 3. Compared to the control, LJT significantly reduced the protein expression of MCP-1 and VCAM-1 at 200 and 400 µg/ml and of ICAM-1 at 400 µg/ml. In addition, it increased both KLF2 and eNOS protein levels at 200 and 400 µg/ml. Although LJT did not have an effect on ERK expression in comparison with the control, it significantly reduced JNK levels at 200 and 400 µg/ml and p38 levels at 400 µg/ml. Conclusions: These results suggest that LJT has an effect on the inhibition of inflammatory factor expression associated with atherosclerosis in HUVECs which could contribute to the prevention of cardiovascular and cerebrovascular diseases.
참고문헌 (33)
- Annual report on the causes of death statistics in 2019, "Statistics Korea", Statistics Korea, ~ (2020)
- World Health Organization, "Global Health Observatory (GHO) data", World Health Organization, ~ (2016)
- Fauci A, "Harrison's principles of internal medicine", pp.1815~1819 (2010), MIP
- Jeongdam Editorial Department, "SIM Integrated internal medicine understood through anatomical pathophysiology 3:Cardiology", pp.205~208 (2013), Jeongdam
- Herbalism Compilation Committee, "Herbalism", pp.240~241 (2013), Younglim Inc
- 배지현, "식중독 유발세균의 증식에 미치는 금은화 추출물의 항균효과", 한국식품과학회지, 37(4), pp.642~647 (2005), 한국식품과학회
- 서성숙, "발효 금은화의 인플루엔자 바이러스 A형에 대한 저해효과", 대한한방내과학회지, 30(3), pp.465~480 (2009), 대한한방내과학회
- Kim JW, "Chemopreventive Potential of Lonicerae flos Aqua-Acupuncture Solution", Kor J Pharmacogn, 30(3), pp.261~268 (1999)
- 박희수, "금은화 약침의 항암효과에 관한 연구", Journal of Acupuncture Research, 22(5), pp.91~97 (2005), 대한침구의학회
- 윤용갑, "금은화 수용성 추출물의 LPS 유도 염증매개물 억제 효과", 대한본초학회지, 22(3), pp.117~125 (2007), 대한본초학회
- 윤경진, "금은화 추출액이 RAW 264.7 Macrophage에서의 NO와 PGE2 생성에 미치는 영향", Journal of Acupuncture Research, 29(1), pp.67~74 (2012), 대한침구의학회
- 주정석, "소회향과 금은화 추출물의 급성췌장염에 대한 예방효과", 대한본초학회지, 25(4), pp.39~45 (2010), 대한본초학회
- 이연우, "항염증조절을 통한 금은화-감초 복합 추출물의 DSS 유도 궤양성 대장염 완화 효과", 대한한방소아과학회지, 32(3), pp.16~25 (2018), 대한한방소아과학회[DOI]
- 주신탁, "Lonicera Flower의 항산화활성과 LDL 산화 억제효과 및 혈전용해능에 관한 연구", 동의생리병리학회지, 22(6), pp.1509~1517 (2008), 한의병리학회
- Choi YS, "Textbook of Cardiovascular Medicine", pp.421~431 (2010), Iljogak
- Kim CJ, "Atherosclerosis and Inflammation", Kor J Lipidology, 11(4), pp.413~419 (2001)
- Bergheanu SC, "Pathophysiology and treatment of atherosclerosis", Neth Heart J, 25(4), pp.231~242 (2017)
- 김상현, "이상지질혈증의 약물치료", 대한의사협회지, 59(5), pp.366~373 (2016), 대한의사협회[DOI]
- Committee for Guidelines for Management of Dyslipidemia, "2015 Korean Guideline for Management of Dyslipidemia", J Lipid Atheroscler, 4(1), pp.61~92 (2015)
- 최희정, "스타틴 치료 시 부작용에 대한 전략", Korean Journal of Family Medicine, 25(10), pp.713~720 (2004), 대한가정의학회
- Mihaylova B, "The effects of lowering LDL cholesterol with statin therapy in people at low risk of vascular disease:Meta-analysis of individual data from 27 randomised trials", Lancet, 380(9841), pp.581~590 (2012)
- Park HJ, "Human Umbilical Vein Endothelial Cells and Human Dermal Microvascular Endothelial Cells Offer New Insights Into the Relationship Between Lipid Metabolism and Angiogenesis", Stem Cell Rev, 2(2), pp.93~102 (2006)
- Han JM, "Soy-Leaf Extract Exerts Atheroprotective Effects via Modulation of Krűppel-Like Factor 2 and Adhesion Molecules", Int J Mol Sci, 18(2), pp.373~ (2017)
- Christopherson K, "Chemokine Regulation of Normal and Pathologic Immune Responses", Stem Cells, 19(5), pp.388~396 (2001)
- Feinberg MW, "An emerging role for Krűppel-like factors in vascular biology", Trends Cardiovasc Med, 14(6), pp.241~246 (2004)
- Channon KM, "Nitric Oxide Synthase in Atherosclerosis and Vascular Injury:insights from experimental gene therapy", Arterioscler Thromb Vasc Biol, 20(8), pp.1873~1881 (2000)
- Johnson GL, "Mitogen-Activated Protein Kinase Pathways Mediated by ERK, JNK, and p38 Protein Kinases", Science, 298(5600), pp.1911~1912 (2002)
- Parravicini V, "Fyn kinase initiates complementary signals required for IgE-dependent mast cell degranulation", Nat Immunol, 3(8), pp.741~748 (2002)
- Cohen MP, "Glycated albumin increases oxidative stress, activates NF-κB and extracellular signal-regulated kinase(ERK), and stimulates ERK-dependent transforming growth factor-β1 production in macrophage RAW cells", J Lab Clin Med, 141(4), pp.242~249 (2003)
- Song HY, "Tumor necrosis factor(TNF)- mediated kinases cascades:Bifurcation of Nuclear Factor-κB and c-Jun N terminal kinases(JNK", SARK) pathways at TNF receptor-assoiated factor 2, 1997, pp.94(18), 9792~9796
- Grynberg K, "The JNK Signaling Pathway in Renal Fibrosis", Front Phys, 8, pp.829~ (2017)
- Dean JLE, "p38 mitogen activated protein kinase regulates cycloxygenase-2 mRNA stability and transcription in lipopolysaccaride treated human monocytes", J Biol chem, 274(1), pp.264~269 (1999)
- Saklatvala J, "The p38 MAP kinase pathway as a therapeutic target in inflammatory disease", Curr Opin Pharmacol, 4(4), pp.372~377 (2004)