갯씀바귀(Ixeris repens) 추출물의 항산화성 및 생리활성
Comparison of Antioxidant and Physiological Activities in Various Solvent Extracts of Ixeris repens
2
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
Ixeris repens is a type of halophyte that grows in high salinity sands found in coastal sand dunes and sandy shores. This study was conducted to investigate the contents, antioxidant potency, and physiological activities of I. repens. In analyses of general composition, carbohydrate, protein, ash, and moisture content were 57.42%, 10.48%, 11.99% and 10.29%, respectively. Potassium, calcium, sodium, and magnesium were its most prevalent minerals. The solvents used to extract I. repens were 70% ethanol, 80% methanol, and distilled water. Among the resultant extracts, ethanol and methanol extracts displayed higher total polyphenol and flavonoid contents than the water extract. ABTS (IC50, 0.12 mg/mL) and FRAP (0.77 mM) radical scavenging activity were highest in the water extract, while methanol extract exhibited the strongest DPPH radical scavenging activity (IC50, 1.32 mg/mL), NO scavenging activity (IC50, 4.10 mg/mL), and reducing power (EC50, 0.14 mg/mL). Tyrosinase, elastase, and α-glucosidase inhibitory activities were highest in the ethanol extract. The ethanol extract also possessed the most potent acetylcholinesterase inhibitory activity. These results indicate that I. repens may be useful as an antioxidant, and a functional substance in food and pharmaceutical materials.
Abstract
Ixeris repens is a type of halophyte that grows in high salinity sands found in coastal sand dunes and sandy shores. This study was conducted to investigate the contents, antioxidant potency, and physiological activities of I. repens. In analyses of general composition, carbohydrate, protein, ash, and moisture content were 57.42%, 10.48%, 11.99% and 10.29%, respectively. Potassium, calcium, sodium, and magnesium were its most prevalent minerals. The solvents used to extract I. repens were 70% ethanol, 80% methanol, and distilled water. Among the resultant extracts, ethanol and methanol extracts displayed higher total polyphenol and flavonoid contents than the water extract. ABTS (IC50, 0.12 mg/mL) and FRAP (0.77 mM) radical scavenging activity were highest in the water extract, while methanol extract exhibited the strongest DPPH radical scavenging activity (IC50, 1.32 mg/mL), NO scavenging activity (IC50, 4.10 mg/mL), and reducing power (EC50, 0.14 mg/mL). Tyrosinase, elastase, and α-glucosidase inhibitory activities were highest in the ethanol extract. The ethanol extract also possessed the most potent acetylcholinesterase inhibitory activity. These results indicate that I. repens may be useful as an antioxidant, and a functional substance in food and pharmaceutical materials.
참고문헌 (37)
- Md. Ali, "α-Glucosidase and Protein Tyrosine Phosphatase 1B Inhibitory Activity of Plastoquinones from Marine Brown Alga Sargassum serratifolium", Marine Drugs, 15(12), pp.368~ (2017), MDPI AG[DOI]
- AOAC(Association of Official Analytical Chemists), "Official methods of analysis. Association of Official Analytical Chemists. 15th ed", 777~788 (1990)
- Iris F.F. Benzie, "The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay", Analytical Biochemistry, 239(1), pp.70~76 (1996), Elsevier BV[DOI]
- Byun HG, "Development of physiological activity and health functional materials of seaweed", Susanyeon-gu, 22, pp.1~10 (2005)
- 조은경, "감태 열수 추출물의 생리활성", 생명과학회지, 20(11), pp.1675~1682 (2010), 한국생명과학회
- 최재홍, "자색 고구마추출물의 기능성 화장품 활성", Food Science and Preservation, 18(3), pp.414~422 (2011), 한국식품저장유통학회
- George L. Ellman, "A new and rapid colorimetric determination of acetylcholinesterase activity", Biochemical Pharmacology, 7(2), pp.88~95 (1961), Elsevier BV[DOI]
- 황숭택, "칠면초(Suaeda japonica) expansin 유전자의 분리 및 특성 분석", 생명과학회지, 23(2), pp.182~189 (2013), 한국생명과학회[DOI]
- Koichi Iida, "Potent Inhibitors of Tyrosinase Activity and Melanin Biosynthesis fromRheum officinale", Planta Medica, 61(05), pp.425~428 (1995), Georg Thieme Verlag KG[DOI]
- 전명제, "톳(Hizikia fusiformis) 에탄올 추출물 및 분획물의 미백활성", 생명과학회지, 22(7), pp.889~896 (2012), 한국생명과학회[DOI]
- Chanipa Jiratchayamaethasakul, "In vitro screening of elastase, collagenase, hyaluronidase, and tyrosinase inhibitory and antioxidant activities of 22 halophyte plant extracts for novel cosmeceuticals", Fisheries and Aquatic Sciences, 23(1), pp.1~9 (2020), 한국수산과학회[DOI]
- 김순석, "태안에서 서식하는 사구식물 4종의 폴리페놀, 플라보노이드 함량과항산화 활성 비교", 한국자원식물학회지, 30(1), pp.8~16 (2017), 한국자원식물학회[DOI]
- 김풍호, "멍게(Halocynthia roretzi) 식해 및 조미 멍게의 영양 및 생리활성 특성", 한국수산과학회지, 47(1), pp.1~11 (2014), 한국수산과학회[DOI]
- 김명조, "씀바귀(Ixeris dentata Nakai) 생즙 추출물의 생리활성", 한국식품영양과학회지, 31(5), pp.924~930 (2002), 한국식품영양과학회
- 김명조, "씀바귀 뿌리 추출물의 항상화성, 항돌연변이원성 및 항암활성 효과", 한국약용작물학회지, 10(3), pp.222~229 (2002), 한국약용작물학회
- Kim S, "Inhibitory effects of Ixeris dentata on the mutagenicity of aflatoxin B1, N-methyl-N'-nitro-N-nitrosoguanidine and the growth of MG-63 human osteosarcoma cells", J Kor Soc Food Nutr, 24, pp.305~312 (1995)
- James A. E. Kraunsoe, "Inhibition of Human Leukocyte and Porcine Pancreatic Elastase by Homologues of Bovine Pancreatic Trypsin Inhibitor", Biochemistry, 35(28), pp.9090~9096 (1996), American Chemical Society (ACS)[DOI]
- 이가순, "열처리 가공조건에 따른 씀바귀 침출차의 생리활성", 한국식품영양과학회지, 38(4), pp.496~501 (2009), 한국식품영양과학회
- Lee SH, "Current status and prospect of nutraceuticals from marine algae", Bull Food Tech, 24, pp.165~175 (2011)
- Lee SY., "Analysis of skin mechanism and an application for functional cosmetic from red bean Phaseolus angularis shell extract", Ph.D., pp.2014
- 이연지, "추출용매에 따른 톳(Hizikia fusiformis) 추출물의 항산화 및 생리활성 비교", 한국수산과학회지, 53(6), pp.886~893 (2020), 한국수산과학회[DOI]
- 이연지, "다시마(Saccharina japonica) 분말을 첨가한 죽의 품질특성 및 항산화 효과", 한국수산과학회지, 50(6), pp.707~713 (2017), 한국수산과학회[DOI]
- 이연지, "톳(Hizikia fusiformis)을 첨가한 흰찰쌀보리죽의 제조 및 품질특성", 한국수산과학회지, 49(3), pp.310~316 (2016), 한국수산과학회
- Lim S, "A Sensory evaluation of the bitter compounds from Ixeris dentata Nakai", J Korean Soc Food Cook Sci, 12, pp.115~121 (1996)
- Middleton E, "Potential health-promoting properties of citrus flavonoids", Food Technol, 48, pp.115~119 (1994)
- Marı́a I.Nieva Moreno, "Comparison of the free radical-scavenging activity of propolis from several regions of Argentina", Journal of Ethnopharmacology, 71(1-2), pp.109~114 (2000), Elsevier BV[DOI]
- N Oyaizu, "(NZW x BXSB)F1 mouse. A new animal model of idiopathic thrombocytopenic purpura", Journal of Experimental Medicine, 167(6), pp.2017~2022 (1988), Rockefeller University Press[DOI]
- Roberta Re, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radical Biology and Medicine, 26(9-10), pp.1231~1237 (1999), Elsevier BV[DOI]
- MAMORU SETO, "Sesquiterpene lactones from Ixeris dentata Nakai", Chemical and Pharmaceutical Bulletin, 34(10), pp.4170~4176 (1986), Pharmaceutical Society of Japan[DOI]
- Kalidas Shetty, "Prevention of Vitrification Aßociated with in vitro Shoot Culture of Oregano. (Origanum vulgare) by Pseudomonas spp.", Journal of Plant Physiology, 147(3-4), pp.447~451 (1995), Elsevier BV[DOI]
- Soka T, "Shanghai science technology Shogakukan", pp.2243~2246 (1985), Shogakukan Press
- 손현정, "해조류 추출물의 In Vitro 항치매 활성", 한국식품영양과학회지, 45(7), pp.966~972 (2016), 한국식품영양과학회
- 송정은, "Nutritional Characteristics and Physiological Functionality of Antidementia Acetylcholinesterase Inhibitor-containing Methanol Extract from Sorghum bicolor", 한국식품영양학회지, 23(2), pp.226~232 (2010), 한국식품영양학회
- Vincenzo Nicola Talesa, "Acetylcholinesterase in Alzheimer's disease", Mechanisms of Ageing and Development, 122(16), pp.1961~1969 (2001), Elsevier BV[DOI]
- Jun Watanabe, "Isolation and Identification ofα-Glucosidase Inhibitors from Tochu-cha (Eucommia ulmoides)", Bioscience, Biotechnology, and Biochemistry, 61(1), pp.177~178 (1997), Oxford University Press (OUP)[DOI]
- 楊希, "다양한 염생식물의 생리활성 평가 및 칠면조로부터 항산화 활성 물질의 분리와 구조해석", 석사, 식품공학, pp.2011
- Yook C, "Colored medicinal plants", pp.588~589 (1997), Korea Academybook