본문 바로가기
KERD
논문

Lauric acid reduces apoptosis by inhibiting FOXO3a-signaling in deoxynivalenol-treated IPEC-J2 cells

0

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류축산학

초록

Deoxynivalenol (DON) is the most common mycotoxin contaminant of food or feed worldwide and causes disease in animals. Lauric acid (LA) is a medium-chain fatty acid useful for barrier functions such as antimicrobial activity in the intestine of monogastric animals. However, the molecular mechanisms by which lauric acid exerts its effects on the deoxynivalenolexposed small intestine have not been studied. We used an intestinal porcine epithelial cell line (IPEC-J2) as an in vitro model to explore the molecular mechanism of lauric acid in alleviating deoxynivalenol-induced damage. We found that lauric acid reversed deoxynivalenol-induced reduction in cell viability. Our quantitative real-time polymerase chain reaction results indicated that lauric acid alleviated deoxynivalenol-induced apoptosis through Annexin-V. Additionally, immunofluorescence and Western blotting showed that lauric acid attenuated deoxynivalenol-induced forkhead box O3 (FOXO3a) translocation into the nucleus. These results suggest that lauric acid attenuates forkhead box O3 translocation in the small intestine damaged by deoxynivalenol, thereby reducing apoptosis. In conclusion, this study found that lauric acid alleviates deoxynivalenol-induced damage in intestinal porcine epithelial cell line through various molecular mechanisms.

Abstract

Deoxynivalenol (DON) is the most common mycotoxin contaminant of food or feed worldwide and causes disease in animals. Lauric acid (LA) is a medium-chain fatty acid useful for barrier functions such as antimicrobial activity in the intestine of monogastric animals. However, the molecular mechanisms by which lauric acid exerts its effects on the deoxynivalenolexposed small intestine have not been studied. We used an intestinal porcine epithelial cell line (IPEC-J2) as an in vitro model to explore the molecular mechanism of lauric acid in alleviating deoxynivalenol-induced damage. We found that lauric acid reversed deoxynivalenol-induced reduction in cell viability. Our quantitative real-time polymerase chain reaction results indicated that lauric acid alleviated deoxynivalenol-induced apoptosis through Annexin-V. Additionally, immunofluorescence and Western blotting showed that lauric acid attenuated deoxynivalenol-induced forkhead box O3 (FOXO3a) translocation into the nucleus. These results suggest that lauric acid attenuates forkhead box O3 translocation in the small intestine damaged by deoxynivalenol, thereby reducing apoptosis. In conclusion, this study found that lauric acid alleviates deoxynivalenol-induced damage in intestinal porcine epithelial cell line through various molecular mechanisms.

참고문헌 (44)

  1. Yang H, "Effects of weaning on intestinal upper villus epithelial cells of piglets", PLOS ONE, 11, pp.e0150216~ (2016)[DOI]
  2. Jang HJ, "MicroRNA expression profiling during the suckling-to-weaning transition in pigs", J Anim Sci Technol, 63, pp.854~863 (2021)[DOI]
  3. Kang R, "Deoxynivalenol induced apoptosis and inflammation of IPEC-J2 cells by promoting ROS production", Environ Pollut, 251, pp.689~698 (2019)[DOI]
  4. Yi H, "Cathelicidin-WA improves intestinal epithelial barrier function and enhances host defense against enterohemorrhagic Escherichia coli O157 : H7infection", J Immunol, 198, pp.1696~1705 (2017)[DOI]
  5. Mun D, "Effects of Bacillus-based probiotics on growth performance, nutrient digestibility, and intestinal health of weaned pigs", J Anim Sci Technol, 63, pp.1314~1327 (2021)[DOI]
  6. Gallo RL, "Epithelial antimicrobial defence of the skin and intestine", Nat Rev Immunol, 12, pp.503~516 (2012)[DOI]
  7. Ren Z, "Progress in mycotoxins affecting intestinal mucosal barrier function", Int J Mol Sci, 20, pp.2777~ (2019)[DOI]
  8. Gao Y, "The compromised intestinal barrier induced by mycotoxins", Toxins, 12, pp.619~ (2020)[DOI]
  9. Zhang C, "Deoxynivalenol triggers porcine intestinal tight junction disorder : insights from mitochondrial dynamics and mitophagy", Ecotoxicol Environ Saf, 248, pp.114291~ (2022)[DOI]
  10. Liao S, "Chloroquine improves deoxynivalenol-induced inflammatory response and intestinal mucosal damage in piglets", Oxid Med Cell Longev, 2020, pp.9834813~ (2020)[DOI]
  11. García GR, "Intestinal toxicity of deoxynivalenol is limited by Lactobacillus rhamnosus RC007 in pig jejunum explants", Arch Toxicol, 92, pp.983~993 (2018)[DOI]
  12. Wang S, "Deoxynivalenol impairs porcine intestinal host defense peptide expression in weaned piglets and IPEC-J2 cells", Toxins, 10, pp.541~ (2018)[DOI]
  13. Yao Y, "The biological detoxification of deoxynivalenol : a review", Food Chem Toxicol, 145, pp.111649~ (2020)[DOI]
  14. Čolović R, "Decontamination of mycotoxin-contaminated feedstuffs and compound feed", Toxins, 11, pp.617~ (2019)[DOI]
  15. Hussein HS, "Toxicity, metabolism, and impact of mycotoxins on humans and animals", Toxicology, 167, pp.101~134 (2001)[DOI]
  16. Yang Y, "The effects of lauric acid on IPEC-J2 cell differentiation, proliferation, and death", Curr Mol Med, 20, pp.572~581 (2020)[DOI]
  17. Surendra KC, "Rethinking organic wastes bioconversion : evaluating the potential of the black soldier fly(Hermetia illucens(L. ))(Diptera : stratiomyidae)(BSF)", Waste Manag, 117, pp.58~80 (2020)[DOI]
  18. Jia M, "Effects of medium chain fatty acids on intestinal health of monogastric animals", Curr Protein Pept Sci, 21, pp.777~784 (2020)[DOI]
  19. Lauridsen C, "Effects of dietary fatty acids on gut health and function of pigs pre- and postweaning", J Anim Sci, 98, pp.skaa086~ (2020)[DOI]
  20. Jackman JA, "Medium-chain fatty acids and monoglycerides as feed additives for pig production : towards gut health improvement and feed pathogen mitigation", J Anim Sci Biotechnol, 11, pp.44~ (2020)[DOI]
  21. Zheng C, "Effects of a combination of lauric acid monoglyceride and cinnamaldehyde on growth performance, gut morphology, and gut microbiota of yellow-feathered broilers", Poult Sci, 102, pp.102825~ (2023)[DOI]
  22. Vandenbroucke V, "The mycotoxin deoxynivalenol potentiates intestinal inflammation by Salmonella typhimurium in porcine ileal loops", PLOS ONE, 6, pp.e23871~ (2011)[DOI]
  23. Chen J, "Plant-derived polyphenols as Nrf2activators to counteract oxidative stress and intestinal toxicity induced by deoxynivalenol in swine : an emerging research direction", Antioxidants, 11, pp.2379~ (2022)[DOI]
  24. Yoon JW, "Gene expression profiling after ochratoxin a treatment in small intestinal epithelial cells from pigs", J Anim Sci Technol, 64, pp.842~853 (2022)[DOI]
  25. Tang M, "Berberine improves intestinal barrier function and reduces inflammation, immunosuppression, and oxidative stress by regulating the NF-κB", MAPK signaling pathway in deoxynivalenol-challenged piglets, 2021, pp.289, 117865~
  26. Tham YY, "Lauric acid alleviates insulin resistance by improving mitochondrial biogenesis in THP-1 macrophages", Mol Biol Rep, 47, pp.9595~9607 (2020)[DOI]
  27. Wu Y, "Serum metabolome and gut microbiome alterations in broiler chickens supplemented with lauric acid", Poult Sci, 100, pp.101315~ (2021)[DOI]
  28. Zeng X, "Dietary butyrate, lauric acid and stearic acid improve gut morphology and epithelial cell turnover in weaned piglets", Anim Nutr, 11, pp.276~282 (2022)[DOI]
  29. Liu Z, "Lauric acid alleviates deoxynivalenolinduced intestinal stem cell damage by potentiating the Akt", mTORC1, Chem Biol Interact, pp.2021, 348[DOI]
  30. Wang X, "Deoxynivalenol induces inflammatory injury in IPEC-J2 Cells via NF-κB signaling pathway", Toxins, 11, pp.733~ (2019)[DOI]
  31. Xu X, "Apoptosis and apoptotic body: disease message and therapeutic target potentials", Biosci Rep, 39, pp.BSR20180992~ (2019)[DOI]
  32. Negroni A, "Apoptosis, necrosis, and necroptosis in the gut and intestinal homeostasis", Mediators Inflamm, 2015, pp.250762~ (2015)[DOI]
  33. Kang TH, "Deoxynivalenol induces apoptosis via FOXO3a-signaling pathway in small-intestinal cells in pig", Toxics, 10, pp.535~ (2022)[DOI]
  34. Leard LE, "Mesothelial cell proliferation and apoptosis", Respirology, 9, pp.292~299 (2004)[DOI]
  35. Zhao W, "Liraglutide inhibits the proliferation and promotes the apoptosis of MCF-7 human breast cancer cells through downregulation of microRNA-27a expression", Mol Med Rep, 17, pp.5202~5212 (2018)[DOI]
  36. Hagenbuchner J, "Mitochondria and FOXO3 : breath or die", Front Physiol, 4, pp.147~ (2013)[DOI]
  37. Melnik BC, "Apoptosis may explain the pharmacological mode of action and adverse effects of isotretinoin, including teratogenicity", Acta Derm Venereol, 97, pp.173~181 (2017)[DOI]
  38. Brunet A, "Transcription-dependent and-independent control of neuronal survival by the PI3K–Akt signaling pathway", Curr Opin Neurobiol, 11, pp.297~305 (2001)[DOI]
  39. Eskandari E, "Paradoxical roles of caspase-3 in regulating cell survival, proliferation, and tumorigenesis", J Cell Biol, 221, pp.e202201159~ (2022)[DOI]
  40. Enari M, "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD", Nature, 391, pp.43~50 (1998)[DOI]
  41. Larsen BD, "The caspase-activated DNase : apoptosis and beyond", FEBS J, 284, pp.1160~1170 (2017)[DOI]
  42. Nikoletopoulou V, "Crosstalk between apoptosis, necrosis and autophagy", Biochim Biophys Acta Mol Cell Res, 1833, pp.3448~3459 (2013)[DOI]
  43. Zhang X, "Akt, FoxO and regulation of apoptosis", Biochim Biophys Acta Mol Cell Res, 1813, pp.1978~1986 (2011)[DOI]
  44. Snoeks L, "Tumor suppressor Foxo3a is involved in the regulation of lipopolysaccharide-induced interleukin-8 in intestinal HT-29 cells", Infect Immun, 76, pp.4677~4685 (2008)[DOI]

관련 논문AI추천

Gene expression profiling after ochratoxin A treatment in small intestinal epithelial cells from pigsJung Woong Yoon, Sang In Lee한국축산학회 · 한국축산학회지2022 · 제64권 제5호shRNA-interfered of Nrf2 reveals a critical role for Keap1-Nrf2 signaling pathway during effects of zearalenone induced oxidative stress in IPEC-J2 cellsCheng Qun, Huang Li bo, Jiang Shu zhen, Yang Wei Ren아세아·태평양축산학회 · Animal Bioscience2025 · 제38권 제2호Protective effects of biological feed additives on gut microbiota and the health of pigs exposed to deoxynivalenol: a review박성권, Byeonghyeon Kim, Jin Young Jeong, Min Ji Kim, Neeraja Recharla한국축산학회 · 한국축산학회지2022 · 제64권 제4호Investigation of the efficacy of mycotoxin-detoxifying additive on health and growth of newly-weaned pigs under deoxynivalenol challengesHolanda Debora Muratori, Kim Sung Woo아세아·태평양축산학회 · Animal Bioscience2021 · 제34권 제3호The Effects of Glucagon-like Peptide-2 on the Tight Junction and Barrier Function in IPEC-J2 Cells through Phosphatidylinositol 3-kinase–Protein Kinase B–Mammalian Target of Rapamycin Signaling PathwayChangsong Yu, Gang Jia, Guangmang Liu, Hua Zhao, KangNing Wang, Qiuhong Deng, Xiaoling Chen아세아·태평양축산학회 · Animal Bioscience2016 · 제29권 제5호Effects of a functional fatty acid blend on growth performance, intestinal morphology, and serum profiles in weaned pigletsLi Jiaan, Li Juntao, Ma Yongxi, Mi Yihai, Wang Huakai, Wang Yanan, Xue Yongqiang, Zhang Yu아세아·태평양축산학회 · Animal Bioscience2023 · 제36권 제5호

이 간행물의 다른 논문 (15건)