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Gene expression profiling after ochratoxin A treatment in small intestinal epithelial cells from pigs

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초록

Ochratoxin A (OTA) is a well-known mycotoxin that causes disease through the ingestion ofcontaminated food or feed, for example, in the porcine industry. The intestinal epithelium actsas the first barrier against food contamination. We conducted a study on the exposure of theporcine intestinal epithelium to OTA. We used the intestinal porcine epithelial cell line IPEC-J2as an in vitro model to evaluate the altered molecular mechanisms following OTA exposure. Gene expression profiling revealed that OTA upregulated 782 genes and downregulated 896,totalling 1678 differentially expressed genes. Furthermore, immunofluorescence, quantitativereal-time polymerase chain reaction, and western blotting confirmed that OTA damages thetight junction protein ZO-1. Moreover, OTA activated the expression of inflammatory genes(IL-6, IL-8, IL-10, NF-kB, TLR4, and TNF-α). In summary, this study confirmed that OTA altersvarious molecular mechanisms and has several adverse effects on IPEC-J2 cells.

Abstract

Ochratoxin A (OTA) is a well-known mycotoxin that causes disease through the ingestion ofcontaminated food or feed, for example, in the porcine industry. The intestinal epithelium actsas the first barrier against food contamination. We conducted a study on the exposure of theporcine intestinal epithelium to OTA. We used the intestinal porcine epithelial cell line IPEC-J2as an in vitro model to evaluate the altered molecular mechanisms following OTA exposure. Gene expression profiling revealed that OTA upregulated 782 genes and downregulated 896,totalling 1678 differentially expressed genes. Furthermore, immunofluorescence, quantitativereal-time polymerase chain reaction, and western blotting confirmed that OTA damages thetight junction protein ZO-1. Moreover, OTA activated the expression of inflammatory genes(IL-6, IL-8, IL-10, NF-kB, TLR4, and TNF-α). In summary, this study confirmed that OTA altersvarious molecular mechanisms and has several adverse effects on IPEC-J2 cells.

참고문헌 (39)

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