저수온기 넙치(Paralichthys olivaceus) 사료 내 가수분해 혈분(Hydrolyzed Blood Meal)의 이용성 평가
Evaluation of Dietary Supplementation with Hydrolyzed Blood Meal for Olive Flounder Paralichthys olivaceus, in Low Water Temperature Conditions
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초록
This study aimed to evaluate the effects of dietary supplementation with two different types of hydrolyzed blood meal (HBM) on the growth performance, feed utilization, digestibility and innate immunity of olive flounder Paralichthys olivaceus. A control diet (Con) consisting of 60% fish meal was formulated and four diets containing two different types of HBM at varying concentrations were prepared 2.5 and 5.0% liquid HBM (L2.5 and L5.0) and 0.5 and 1.0% powdered HBM (P0.5 and P1.0). A total of 450 olive flounder (average body weight: 50±0.07 g) were distributed in 15 tanks (240 L), with three replicate groups per diet. The fish were fed the diets to apparent satiation for 9 weeks and subsequently exposed to Edwardsiella tarda. The results showed that fish fed L2.5, L5.0 and P0.5 diets exhibited significantly higher lysozyme activity compared to those fed the Con and P1.0 diets. During the challenge test against E. tarda, the L5.0 and P0.5 fish groups exhibited higher disease resistance than that of the Con group. These findings indicate that dietary supplementation with HBM could positively effect the innate immunity and disease resistance of olive flounder.
Abstract
This study aimed to evaluate the effects of dietary supplementation with two different types of hydrolyzed blood meal (HBM) on the growth performance, feed utilization, digestibility and innate immunity of olive flounder Paralichthys olivaceus. A control diet (Con) consisting of 60% fish meal was formulated and four diets containing two different types of HBM at varying concentrations were prepared 2.5 and 5.0% liquid HBM (L2.5 and L5.0) and 0.5 and 1.0% powdered HBM (P0.5 and P1.0). A total of 450 olive flounder (average body weight: 50±0.07 g) were distributed in 15 tanks (240 L), with three replicate groups per diet. The fish were fed the diets to apparent satiation for 9 weeks and subsequently exposed to Edwardsiella tarda. The results showed that fish fed L2.5, L5.0 and P0.5 diets exhibited significantly higher lysozyme activity compared to those fed the Con and P1.0 diets. During the challenge test against E. tarda, the L5.0 and P0.5 fish groups exhibited higher disease resistance than that of the Con group. These findings indicate that dietary supplementation with HBM could positively effect the innate immunity and disease resistance of olive flounder.
참고문헌 (40)
- Aladetohun NF, "Utilization of blood meal as a protein ingredient from animal waste product in the diet of Oreochromis niloticus", Int J Fish Aquac, 5, pp.234~237 (2013)[DOI]
- Official Methods of Analysis, "AOAC (Association of Official Analytical Chemists)", Association of Official Analytical Chemists, ~ (2005)
- I.G. Borlongan, "Studies on the digestive lipases of milkfish, Chanos chanos", Aquaculture, 89(3-4), pp.315~325 (1990), Elsevier BV[DOI]
- M Bradford, "A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding", Analytical Biochemistry, 72(1-2), pp.248~254 (1976), Elsevier BV[DOI]
- Hien Thi Dieu Bui, "Growth performance, feed utilization, innate immunity, digestibility and disease resistance of juvenile red seabream (Pagrus major) fed diets supplemented with protein hydrolysates", Aquaculture, 418-419, pp.11~16 (2014), Elsevier BV[DOI]
- Giacomo Canesin, "Heme-Derived Metabolic Signals Dictate Immune Responses", Frontiers in Immunology, 11, pp.66~ (2020), Frontiers Media SA[DOI]
- Rebeca Cerezuela, "Changes in intestinal morphology and microbiota caused by dietary administration of inulin and Bacillus subtilis in gilthead sea bream (Sparus aurata L.) specimens", Fish Shellfish Immunol, 34(5), pp.1063~1070 (2013), Elsevier BV[DOI]
- Clavijo AM, "First report of Edwardsiella tarda from tilapias in Venezuela", Bull Eur Assoc Fish Pathol, 22, pp.280~282 (2002)
- S. Divakaran, "Note on the Methods for Determination of Chromic Oxide in Shrimp Feeds", Journal of Agricultural and Food Chemistry, 50(3), pp.464~467 (2002), American Chemical Society (ACS)[DOI]
- Edeh B, "Blood meal as protein ingredient from animal waste product in the diet of(Oreochromis niloticus)", Adv Aquacult Fish Manage, 2, pp.098~101 (2013)
- Ellis AE, "Techniques in Fish Immunology", pp.95~99 (1990), SOS Publication
- Bernard F. Erlanger, "The preparation and properties of two new chromogenic substrates of trypsin", Archives of Biochemistry and Biophysics, 95(2), pp.271~278 (1961), Elsevier BV[DOI]
- Folch J, "A simple method for the isolation and purification of total lipids from animal tissues", J Biol Chem, 226, pp.497~509 (1957)
- Enric Gisbert, "Porcine Protein Hydrolysates (PEPTEIVA®) Promote Growth and Enhance Systemic Immunity in Gilthead Sea Bream (Sparus aurata)", Animals, 11(7), pp.2122~ (2021), MDPI AG[DOI]
- E. Gisbert, "Spray-dried plasma promotes growth, modulates the activity of antioxidant defenses, and enhances the immune status of gilthead sea bream (Sparus aurata) fingerlings1", Journal of Animal Science, 93(1), pp.278~286 (2015), Oxford University Press (OUP)[DOI]
- Buddhi E. Gunathilaka, "Evaluation of shrimp or tilapia protein hydrolysate at graded dosages in low fish meal diet for olive flounder (Paralichthys olivaceus)", Aquaculture Nutrition, 26(5), pp.1592~1603 (2020), Hindawi Limited[DOI]
- Hamed SS, "Effects of blood meal as a substitute for fish meal in the culture of juvenile Silver Pompano Trachinotus blochii(Lacepède, 1801)in a circulating aquaculture system", West Indian Ocean J Mar Sci, 16, pp.1~11 (2017)
- M.C Hidalgo, "Comparative study of digestive enzymes in fish with different nutritional habits. Proteolytic and amylase activities", Aquaculture, 170(3-4), pp.267~283 (1999), Elsevier BV[DOI]
- Nakahiro Iwata, "Effects of Temperature on the Growth of Japanese Flounder", Fisheries science, 60(5), pp.527~531 (1994), Japanese Society of Fisheries Science[DOI]
- Mengru Jin, "Edwardsiella piscicida YccA: A novel virulence factor essential to membrane integrity, mobility, host infection, and host immune response", Fish Shellfish Immunol, 126, pp.318~326 (2022), Elsevier BV[DOI]
- Kasun Tharaka, "Evaluation of Antarctic kirll (Euphausia superba) meal supplementation in diets for olive flounder (Paralichthys olivaceus)", Aquaculture Research, 51(6), pp.2291~2302 (2020), Hindawi Limited[DOI]
- Ye Ji Kim, "Antibiotic Susceptibility of Bacterial Pathogens That Infect Olive Flounder (Paralichthys olivaceus) Cultivated in Korea", International Journal of Environmental Research and Public Health, 19(13), pp.8110~ (2022), MDPI AG[DOI]
- Se-Kwon Kim, "Development and biological activities of marine-derived bioactive peptides: A review", Journal of Functional Foods, 2(1), pp.1~9 (2010), Elsevier BV[DOI]
- Sanaz Khosravi, "Dietary supplementation of marine protein hydrolysates in fish-meal based diets for red sea bream (Pagrus major) and olive flounder (Paralichthys olivaceus)", Aquaculture, 435, pp.371~376 (2015), Elsevier BV[DOI]
- "Expenditure Per Aquaculture" (2023)[URL]
- J Kumari, "Dietary β-1,3 glucan potentiates innate immunity and disease resistance of Asian catfish, Clarias batrachus (L.)", Journal of Fish Diseases, 29(2), pp.95~101 (2006), Wiley[DOI]
- Mengqing Liang, "Effects of different levels of fish protein hydrolysate in the diet on the nonspecific immunity of Japanese sea bass, Lateolabrax japonicus (Cuvieret Valenciennes, 1828)", Aquaculture Research, 37(1), pp.102~106 (2006), Hindawi Limited[DOI]
- Yangzhen Li, "Heritability of disease resistance to Edwardsiella tarda in olive flounder (Paralichthys olivaceus)", Aquaculture, 519, pp.734750~ (2020), Elsevier BV[DOI]
- Oscar Martínez-Alvarez, "Protein hydrolysates from animal processing by-products as a source of bioactive molecules with interest in animal feeding: A review", Food Research International, 73, pp.204~212 (2015), Elsevier BV[DOI]
- Haitham H. Mohammed, "The effects of dietary inclusion of a Saccharomyces cerevisiae fermentation product in a commercial catfish ration on growth, immune readiness, and columnaris disease susceptibility", Journal of Applied Aquaculture, 31(3), pp.193~209 (2018), Informa UK Limited[DOI]
- Naima Nedjar-Arroume, "Isolation and characterization of four antibacterial peptides from bovine hemoglobin", Peptides, 27(9), pp.2082~2089 (2006), Elsevier BV[DOI]
- Johnny Ogunji, "Alternative protein source: Acceptability of cow blood meal in place of fish meal assessed via growth, antioxidant enzymes functions and haematological response in Clarias gariepinus (Burchell, 1822)", Aquaculture Research, 52(6), pp.2651~2661 (2021), Hindawi Limited[DOI]
- Johnny O. Ogunji, "Assessing effects of substituting dietary fish meal with boiled donkey and cow blood meal on growth performance and digestive enzyme activities of Clarias gariepinus juvenile", Journal of the World Aquaculture Society, 51(4), pp.1066~1079 (2020), Wiley[DOI]
- Darunee SAE-OUI, "A case of Edwardsiella tarda infection in cultured colored carp Cyprinus carpio", Fish Pathology, 19(3), pp.197~199 (1984), The Japanese Society of Fish Pathology[DOI]
- Muhammad A.B. Siddik, "Enzymatic fish protein hydrolysates in finfish aquaculture: a review", Reviews in Aquaculture, 13(1), pp.406~430 (2020), Wiley[DOI]
- Siwicki AK, "Fish Disease Diagnosis and Prevention Methods", pp.105~112 (1993), Wydawnictwo Instytutu Rybactwa Strodladowego
- Hong-gang Tang, "Effects of fish protein hydrolysate on growth performance and humoral immune response in large yellow croaker (Pseudosciaena crocea R.)", Journal of Zhejiang University SCIENCE B, 9(9), pp.684~690 (2008), Zhejiang University Press[DOI]
- Innocent Twahirwa, "The effect of dietary fish meal replacement with blood meal on growth performance, metabolic activities, antioxidant and innate immune responses of fingerlings black carp, Mylopharyngodon piceus", Aquaculture Research, 52(2), pp.702~714 (2020), Hindawi Limited[DOI]
- Usman Usman, "FERMENTED BLOOD MEAL USE FOR TIGER GROUPER, Epinephelus fuscoguttatus GROW-OUT DIET", Indonesian Aquaculture Journal, 2(1), pp.1~13 (2007), Agency for Marine and Fisheries Research and Development[DOI]
- Worthington CC, "Enzyme Related Biochemical", pp.38~42 (1991), Worthington Biochemical Corporation
