본문 바로가기
KERD
논문KCI 등재

시베리아 철갑상어(Acipenser baerii) 트랜스페린(Transferrin) 유전자의 발생 배 및 초기 자어에서의 발현 특징

Expression Characteristics of Transferrin Gene During Embryo Development and Prolarval Ontogeny in Siberian Sturgeon Acipenser baerii

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류수산학

초록

Serotransferrin cDNA from the Siberian sturgeon Acipenser baerii was isolated and its expression patterns during early life intervals were characterized. It contained an ORF encoding a 708-aa-long polypeptide, including a 19-aa signal peptide. Bioinfomatic analysis and 3D modeling indicated a typical bi-lobal structure with conserved ironcoordinating residues. During embryonic development, the potential transition of maternally provisioned transcripts to zygotically de novo transcribed ones occurred around blastula stage. The transferrin mRNA levels peaked at stages responsible for pronephros, heart and erythropoietic component differentiation. After hatching, the transferrin mRNA expression gradually increased at early ontogenic phases (0 to 3 DPH) corresponding to the periods in which prolarvae exhibited increased blood circulation and liver differentiation. The expression decreased at subsequent stages in which prolarvae exhibited benthic movement. The tissue distribution assay indicated liver-predominant expression at fingerling stage. From the microinjection-based challenge with Aeromonas hydrophila at day-0 and day-7, the transcriptional response was modulated toward upregulation, in which the amounts induced at 6, 12 and 24 HPI were greater in prolarvae injected at day-7 than at day-0. Therefore, transferrin plays important roles in both early development and host protective responses to pathogens in the Siberian sturgeon.

Abstract

Serotransferrin cDNA from the Siberian sturgeon Acipenser baerii was isolated and its expression patterns during early life intervals were characterized. It contained an ORF encoding a 708-aa-long polypeptide, including a 19-aa signal peptide. Bioinfomatic analysis and 3D modeling indicated a typical bi-lobal structure with conserved ironcoordinating residues. During embryonic development, the potential transition of maternally provisioned transcripts to zygotically de novo transcribed ones occurred around blastula stage. The transferrin mRNA levels peaked at stages responsible for pronephros, heart and erythropoietic component differentiation. After hatching, the transferrin mRNA expression gradually increased at early ontogenic phases (0 to 3 DPH) corresponding to the periods in which prolarvae exhibited increased blood circulation and liver differentiation. The expression decreased at subsequent stages in which prolarvae exhibited benthic movement. The tissue distribution assay indicated liver-predominant expression at fingerling stage. From the microinjection-based challenge with Aeromonas hydrophila at day-0 and day-7, the transcriptional response was modulated toward upregulation, in which the amounts induced at 6, 12 and 24 HPI were greater in prolarvae injected at day-7 than at day-0. Therefore, transferrin plays important roles in both early development and host protective responses to pathogens in the Siberian sturgeon.

참고문헌 (40)

  1. Asmamaw B, "Transferrin in fishes : A review article", J Coastal Life Med, 4, pp.176~180 (2016)[DOI]
  2. Cao J, "Functional divergence of the N-lobe and C-lobe of transferrin gene in Pungitius sinensis(Amur Stickleback)", Animals, 12, pp.3458~ (2022)[DOI]
  3. Charlie-Silva I, "Acute-phase proteins during inflammatory reaction by bacterial infection : Fish-model", Sci Rep, 9, pp.4776~ (2019)[DOI]
  4. Chen JH, "cDNA cloning and expression characterization of serum transferrin gene from oriental weatherfish Misgurnus anguillicaudatus", J Fish Biol, 84, pp.885~896 (2014)[DOI]
  5. Chesneau V, "The Siberian Sturgeon (Acipenser baerii, Brandt, 1869) Volume 2 - Farming", pp.373~402 (2018), Springer
  6. Cordero H, "Comparative ontogenetic development of two marine teleosts, gilthead seabream and European sea bass", Dev Comp Immunol, 65, pp.1~7 (2016)[DOI]
  7. Ding Z, "The Megalobrama amblycephala transferrin and transferrin receptor genes : Molecular cloning, characterization and expression during early development and after Aeromonas hydrophila infection", Dev Comp Immunol, 49, pp.290~297 (2015)[DOI]
  8. Esteban MA, "A review of soluble factors and receptors involved in fish skin immunity : The tip of the iceberg", Fish Shellfish Immunol, 145, pp.109311~ (2024)[DOI]
  9. Gisbert E, "The Siberian Sturgeon (Acipenser baerii, Brandt, 1869) Volume 1 - Biology", pp.131~158 (2018), Springer
  10. Gisbert E, "Histochemistry of the development of the digestive system of Siberian sturgeon during early ontogeny", J Fish Biol, 55, pp.596~616 (1999)[DOI]
  11. Gradil AM, "Ontogeny of the immune system in Acipenserid juveniles", Dev Comp Immunol, 44, pp.303~314 (2014)[DOI]
  12. Höhne C, "The immune system of sturgeons and paddlefish(Acipenseriformes) : A review with new data from a chromosome-scale sturgeon genome", Rev Aquac, 13, pp.1709~1729 (2021)[DOI]
  13. Jurecka P, "Differences in growth of Trypanoplasma borreli in carp serum is dependent on transferrin genotype", Fish Shellfish Immunol, 114, pp.58~64 (2021)[DOI]
  14. Kamińska-Gibas T, "The many faces of transferrin : Does genotype modulate immune response?", Fish Shellfish Immunol, 102, pp.511~518 (2020)[DOI]
  15. Kawabata H, "Transferrin and transferrin receptors update", Free Rad Biol Med, 133, pp.46~54 (2019)[DOI]
  16. Kayiş Ş, "Bacterial pathogens and health problems of Acipenser gueldenstaedtii and Acipenser baerii sturgeons reared in the eastern Black Sea region of Turkey", Iran J Vet Res, 18, pp.18~24 (2017)
  17. Kim CH, "Chondrostean sturgeon hepcidin : An evolutionary link between teleost and tetrapod hepcidins", Fish Shellfish Immunol, 88, pp.117~125 (2019)[DOI]
  18. Kim CH, "Subfunctionalization and evolution of liver-expressed antimicrobial peptide 2(LEAP2)isoform genes in Siberian sturgeon(Acipenser baerii), a primitive chondrostean fish species", Fish Shellfish Immunol, 93, pp.161~173 (2019)[DOI]
  19. Kim CH, "Superoxide dismutase multigene family from a primitive chondrostean sturgeon, Acipenser baerii : Molecular characterization, evolution, and antioxidant defense during development and pathogen infection", Antioxidants, 10, pp.232~ (2021)[DOI]
  20. Kim CH, "Transcriptome analysis of maternal gene transcripts in unfertilized eggs of Misgurnus anguillicaudatus and identification of immunerelated maternal genes", Int J Mol Sci, 21, pp.3872~ (2020)[DOI]
  21. Kim EJ, "Anesthetic protocol for microinjection-related handling of Siberian sturgeon(Acipenser baerii;Acipenseriformes)prolarvae", PLoS One, 13, pp.e0209928~ (2018)[DOI]
  22. Lambert LA, "Molecular evolution of the transferrin family and associated receptors", Biochim Biophys Acta Gen Subj, 1820, pp.244~255 (2012)[DOI]
  23. Liu H, "Structure and expression of transferrin gene of channel catfish, Ictalurus punctatus", Fish Shellfish Immunol, 28, pp.159~166 (2010)[DOI]
  24. Luo L, "Molecular characterization and expression analysis of the transferrin gene in amur ide(Leuciscus waleckii)in response to high alkaline stress", J Appl Ani Res, 50, pp.1~12 (2022)[DOI]
  25. Mohd-Padil H, "Phylogenetic analyses uncover a novel clade of transferrin in nonmammalian vertebrates", Mol Biol Evol, 30, pp.894~905 (2013)[DOI]
  26. Mudumana SP, "Expression analyses of zebrafish transferrin, ifabp, and elastase B mRNAs as differentiation markers for the three major endodermal organs : Liver, intestine, and exocrine pancreas", Dev Dyn, 230, pp.165~173 (2004)[DOI]
  27. Neves JV, "Transferrin and ferritin response to bacterial infection : The role of the liver and brain in fish", Dev Comp Immunol, 33, pp.848~857 (2009)[DOI]
  28. Park CH, "Embryonic development of Siberian sturgeon Acipenser baerii under hatchery conditions : An image guide with embryological descriptions", J Fish Aquat Sci, 16, pp.15~23 (2013)[DOI]
  29. Park CH, "Effects of incubation temperature on egg development, hatching and pigment plug evacuation in farmed Siberian sturgeon Acipenser baerii", Fish Aquat Sci, 16, pp.25~34 (2013)[DOI]
  30. Reyes-Becerril M, "Changes in transferrin gene expression after exposure to iron and Aeromonas hydrophila infection in yellow snapper(Lutjanus argentiventris)", Agri Gene, 1, pp.79~87 (2016)[DOI]
  31. Schmittgen TD, "Analyzing real-time PCR data by the comparative CT method", Nat Protoc, 3, pp.1101~1108 (2008)[DOI]
  32. Stafford JL, "Transferrin and the innate immune response of fish : Identification of a novel mechanism of macrophage activation", Dev Comp Immunol, 27, pp.539~554 (2003)[DOI]
  33. Stafford JL, "Products of proteolytic cleavage of transferrin induce nitric oxide response of goldfish macrophages", Dev Comp Immunol, 25, pp.101~115 (2001)[DOI]
  34. Sun W, "Acute phase response in zebrafish embryo", larva with special emphasis on LPS-induced changes in expression pattern of acute phase protein genes, 2014, pp.94, 1569~1580
  35. Teng T, "Molecular cloning and expression analysis of Megalobrama amblycephala transferrin gene and effects of exposure to iron and infection with Aeromonas hydrophila", Fish Physiol Biochem, 43, pp.987~997 (2017)[DOI]
  36. Valipour A, "Changes in immune parameters(lysozyme, IgM, C3)in early life stages and broodstock of Siberian sturgeon, Acipenser baerii", Fish Aquat Life, 26, pp.21~30 (2018)[DOI]
  37. Wang ZS, "The cDNA sequence of the transferrin gene in tongue sole(Cynoglossus semilaevis)and its transcriptional changes under acute hypoxic stress", Ital J Anim Sci, 11, pp.e74~ (2012)[DOI]
  38. Xin M, "Transferrin identification in sterlet(Acipenser ruthenus)reproductive system", Animals, 9, pp.753~ (2019)[DOI]
  39. Yin X, "Expression and functional characterization of transferrin in Nile tilapia(Oreochromis niloticus)in response to bacterial infection", Fish Shellfish Immunol, 74, pp.530~539 (2018)[DOI]
  40. Yin X, "Purification and functional characterization of serum transferrin from Nile tilapia(Oreochromis niloticus)", Fish Shellfish Immunol, 88, pp.36~46 (2019)[DOI]

관련 논문AI추천

돌돔 ferritin H 유전자의 클로닝과 발현 분석권문경, 김주원, 박찬일, 전태훈, 정지민, 최인호수산생명의학회 · 한국어병학회지2013 · 제26권 제3호Transcriptional Onset of Lysozyme Genes during Early Development in Olive Flounder (Paralichthys olivaceus)김경길, 김현철, 노재구, 박종원, 박철지, 이장욱, 이정호한국발생생물학회 · 발생과 생식2014 · 제18권 제4호The Expression Analysis of Complement Component C3 during Early Developmental Stages in Olive Flounder (Paralichthys olivaceus)노재구, 박철지, Hyun Chul Kim, In Joon Hwang, Jang-Wook Lee, Jeong-HoLee, Jong Won Park, Sung Yeon Kim, Young Mee Lee한국발생생물학회 · 발생과 생식2013 · 제17권 제4호Embryonic Development of Siberian Sturgeon Acipenser baerii under Hatchery Conditions: An Image Guide with Embryological Descriptions김동수, 남윤권, 박철홍, 이상윤한국수산과학회 · Fisheries and Aquatic Sciences2013 · 제16권 제1호Expression of Immune-Related Genes during Loach (Misgurnus anguillicaudatus) Embryonic and Early Larval Development이정호, Hye-Sung Choi, In Bon Goo, Jea Hyun Im, Jeong-Ho Lee, Ju-Ae Hwang, Jung Eun Kim한국발생생물학회 · 발생과 생식2015 · 제19권 제4호Gene structure and expression characteristics of liver-expressed antimicrobial peptide-2 isoforms in mud loach (Misgurnus mizolepis, Cypriniformes)남기완, 이상윤한국수산과학회 · Fisheries and Aquatic Sciences2017 · 제20권 제4호

이 간행물의 다른 논문 (9건)