Anti-Inflammatory Effects of Grasshopper Ketone from Sargassum fulvellum Ethanol Extract on Lipopolysaccharide-Induced Inflammatory Responses in RAW 264.7 Cells
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초록
This study evaluated the anti-inflammatory potential of a grasshopper ketone (GK) isolated from the brown alga Sargassum fulvellum on lipopolysaccharide (LPS)-induced RAW 264.7 murine macrophage cell line. GK was isolated and purified from the n-hexane fraction and its structure was verified on the basis of NMR spectroscopic data. GK up to 100 μg/ml is not cytotoxic to RAW 264.7, and is an effective inhibitor of LPS-induced NO production in RAW 264.7 cells. The production of pro-inflammatory cytokines, including IL-6, IL-1β, and TNF-α was found significantly reduced in 0.1-100 μg/ml dose ranges of GK treatment (p < 0.05). We confirmed the dose-dependent and significant inhibition of iNOS and COX-2 proteins expression. In addition, it has been shown that GK induces anti-inflammatory effects by inhibiting MAPKs (ERK, JNK, and p38) and NF-κB p65 phosphorylation. Our results show that the anti-inflammatory properties of GK may be due to the inhibition of the NF-κB and MAPKs pathways, which are associated with the attenuation of cytokine secretion.
Abstract
This study evaluated the anti-inflammatory potential of a grasshopper ketone (GK) isolated from the brown alga Sargassum fulvellum on lipopolysaccharide (LPS)-induced RAW 264.7 murine macrophage cell line. GK was isolated and purified from the n-hexane fraction and its structure was verified on the basis of NMR spectroscopic data. GK up to 100 μg/ml is not cytotoxic to RAW 264.7, and is an effective inhibitor of LPS-induced NO production in RAW 264.7 cells. The production of pro-inflammatory cytokines, including IL-6, IL-1β, and TNF-α was found significantly reduced in 0.1-100 μg/ml dose ranges of GK treatment (p < 0.05). We confirmed the dose-dependent and significant inhibition of iNOS and COX-2 proteins expression. In addition, it has been shown that GK induces anti-inflammatory effects by inhibiting MAPKs (ERK, JNK, and p38) and NF-κB p65 phosphorylation. Our results show that the anti-inflammatory properties of GK may be due to the inhibition of the NF-κB and MAPKs pathways, which are associated with the attenuation of cytokine secretion.
참고문헌 (24)
- 김보운, "LPS로 유도된 RAW 264.7 대식세포에 대한 애기외톨개 모자반(Myagropsis yendoi) 에틸아세테이트 분획물의 항염증 효과", 한국수산과학회지, 47(5), pp.527~536 (2014), 한국수산과학회
- Lawrence T, "Antiinflammatory lipid mediators and insights into the resolution of inflammation", Nat. Rev. Immunol, 2, pp.787~795 (2002)
- Ljung T, "Rectal nitric oxide as biomarker in the treatment of inflammatory bowel disease: responders versus non-responders", World J. Gastroenterol, 12, pp.3386~3392 (2006)
- 곽충실, "한국산 5종 해조류의 항산화효과와 총 폴리페놀 함량과의 관련성", 한국식품영양과학회지, 34(8), pp.1143~1150 (2005), 한국식품영양과학회
- 김수현, "Antioxidant Activity of Sulfated Polysaccharides Isolated from Sargassum fulvellum", Preventive Nutrition and Food Science, 12(2), pp.65~73 (2007), 한국식품영양과학회
- 강수희, "참모자반 메탄올 추출 분획물의 항산화 및 숙취해소능과 α-glucosidase 활성저해효과", 생명과학회지, 22(10), pp.1420~1427 (2012), 한국생명과학회[DOI]
- Lee BH, "Extraction of water soluble antioxidants from seaweeds", J. Korean Ind. Eng. Chem, 7, pp.1069~1077 (1996)
- Donguibogam Committee, "Translated donguibogam", pp.2198~ (1996), Bubinmunwha Press
- 배송자, "모자반 분획물의 in vitro에서의 항발암효과", 한국식품영양과학회지, 33(3), pp.480~486 (2004), 한국식품영양과학회
- Lee HS, "Preparation of antibacterial agent from seaweed extract and its antibacterial effect", J. Korean Fish Soc, 33, pp.32~37 (2000)
- Gray JI, "Inhibition of N-nitrosamine formation in model food systems", J. Food Sci, 40, pp.981~984 (1975)
- Lee AK, "Inhibition of lipopolysaccharide-inducible nitric oxide synthase, TNF-αand COX-2 expression by sauchinone effects on I-κBαphosphorylation, C", EBP and AP-1 activation, 2003, pp.139, 11~20
- Pahan K, "Induction of nitric-oxide synthase and activation of NF-kappaB by interleukin-12 p40 in microgial cells", J. Biol. Chem, 276, pp.7899~7905 (2001)
- Zhang G, "Molecular mechanisms of NF-κB activation induced by bacterial lipopolysaccharide through Toll-like receptors", J. Endotoxin Res, 6, pp.453~457 (2000)
- Jang BC, "Catalase induced expression of inflammatory mediators via activation of NF-κB, PI3K", AKT, p70S6K, and JNKs in BV2 microglia, 2005, pp.17, 625~633
- Majdalawieh A, "Regulation of IκBα function and NF-κB signaling : AEBP1 is a novel pro-inflammatory mediator in macrophages", Mediators Inflamm, 2010, pp.1~27 (2010)
- Heo SJ, "Chromene suppresses the activation of inflammatory mediators in lipopolysaccharide-stimulated RAW 264,7 cells", Food Chem. Toxicol, 67, pp.169~175 (2014)
- Lee JH, "Preparative isolation of sargachromanol E from Sargassum siquastrum by centrifugal partition chromatography and its anti-inflammatory activity", Food Chem. Toxicol, 51, pp.54~60 (2013)
- 한민호, "ICR 마우스를 이용한 오미자 종자 정유의 단회경구투여 독성시험", 생명과학회지, 24(2), pp.191~195 (2014), 한국생명과학회[DOI]
- Tracey D, "Tumor necrosis factor antagonist mechanisms of action : a comprehensive review", Pharmacol. Ther, 117, pp.244~279 (2008)
- 정다현, "LPS로 유도된 RAW 264.7 세포와 마우스 귀조직에 대한 참모자반(Sargassum fulvellum) 에탄올 추출물의 항염증 효과", 한국식품영양과학회지, 43(8), pp.1158~1165 (2014), 한국식품영양과학회
- Yang EJ, "Sargachromenol from Sargassum micracanthum inhibits the lipopolysaccharideinduced production of inflammatory mediators in RAW 264,7 macrophages", Sci. World J, 2013, pp.1~6 (2013)
- Heo SJ, "Anti-inflammatory effect of fucoxanthin derivatives isolated from Sargassum siliquastrum in lipopolysaccharidestimulated RAW 264,7 macrophage", Food Chem. Toxicol, 50, pp.3336~3342 (2012)
- Yang EJ, "Antiinflammatory effects of apo-9’-fucoxanthinone from the brown alga, Sargassum muticum", Daru. J. Pharm. Sci, 21, pp.1~7 (2013)