The Effect of Estrogen Deficiency on Rat Pulpodentinal Complex
The effect of estrogen deficiency on rat pulpodentinal complex
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초록
난소 절제술을 시행한 백서에서 에스트로겐 호르몬의 결핍으로 인한 상아-치수 복합체의 변화를 관찰하였다. 30 마리의 암놈 Sprague-Dawley rats을 두 군으로 나누어 1 군은 Sham- surgery를 시행하였고, 2 군은 양측 난소 절제술을 시행하였다. 백서는 12주 뒤에 모두 희생시켜, 하악 치아와 인접한 치주조직을 포함하여 절제하였고, 10% 중성 포르말린 용액에 고정하였다. 두 군의 차이점을 비교하기 위하여 방사선 사진을 촬영하고, osteonectin을 이용한 면역 화학 염색법을 시행하였다. 조직 형태학적 차이점을 측정하기 위하여 영상 분석 프로그램을 사용하였고, 분석 방법으로는 Paired t-test를 사용하였다(p<0.05). osteonectin을 이용한 면역 화학 염색 결과, 두 군간에는 유의성 있는 차이점이 존재하였다. 난소 절제술을 시행한 백서군에서는 전상아질층의 두께가 현저히 증가하였고, Sham- surgery 군에서는 전상아질 층과 치수 조직에서 osteonectin이 좀 더 특이적으로 염색되어 나타났다. 이러한 결과로 결론지어보면, 백서에서 인위적으로 난소 절제술을 시행하였을 때 광화되지 않은 전상아질층의 두께가 증가하고, 치수 조직과 전상아질층내의 osteonectin 함량이 감소되며, 결국 에스트로겐 결핍은 조상아 세포의 기전을 변화시킨다고 할 수 있다.
Abstract
The purpose of this study was to investigate the effects of estrogen deficiency on pulpodentinal complex of tooth in ovariectomized rats. Thirty female Sprague-Dawley rats, 10 weeks old, were used. Rats were grouped into two groups. One group (n=15) was subjected to sham surgery (SHAM) and the other group (n=15) was ovariectomized bilaterally (OVX). Animals were sacrificed 12 weeks later, and their mandibular molars and associated periodontal supporting tissues were dissected out, and fixed in 10% buffered formalin. For comparison of groups, immunostained for osteonectin. Histomorphometrical measurement of change of teeth was performed using an image analysis system and paired t-test was used and the level of significance for overall differences was set at p<0.05. In immunostaining of osteonectin, they were significantly different from each other. The predentin thickness in OVX rats was wider than in SHAM rats. And in SHAM rats, osteonectin was more specifically stained in predentin areas than in OVX rats. These results indicate that estrogen deficiency increased the unmineralized predentin areas and decreased osteonectin content in pulpal tissues in rats. If our result is applicable to human studies, odotoblast is affected by estrogen deficiency.
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