치주염 유발 쥐에서 상아질파괴세포와 뼈파괴세포의 형성
Odontoclast and Osteoclast Formation in Rats with Ligature-Induced Periodontitis
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초록
치주염을 유발한 쥐 치아의 근심면에서 상아질파괴세포와 뼈파괴세포의 형성을 비교하였다. 뼈파괴세포는 치주염유발 후 3일까지 증가한 후 감소하였으나 상아질파괴세포는 치주염 유발 10일까지 서서히 증가하였으며 치주염 유발전과 후의 상아질파괴세포의 수는 뼈파괴세포보다 적었다. 또한 치근 흡수는 상아질파괴세포가 증가함에 따라 증가하였다. 이들 결과는 치주염 시 상아질파괴세포 형성도 뼈파괴세포처럼 증가하나 뼈파괴세포보다 서서히 약하게 진행됨을 시사한다. 본 연구에서 사용한 동물 모델과 연구 결과는 치주염에서 상아질파괴세포와 뼈파괴세포의 형성이 차이가 있음을 제시하는 최초 보고이며, 이들 세포의 형성 기전의 차이를 규명하는 앞으로의 연구에 유용한 자료로 이용될 수 있을 것으로 판단한다.
Abstract
Odontoclasts and osteoclasts resorb tooth root and alveolar bone, respectively. Many studies have focused on osteoclast formation in periodontitis, but effect of periodontitis on odontoclast formation is not clearly clarified. In this study, we observed formation of odontoclasts as well as osteoclasts in rats with ligature-induced periodontitis. To induce periodontitis, ligatures were placed around the first molars in left mandibles of rats. Rats were sacrificed at days 1, 3, and 10 after ligation. After tartrate resistant acid phosphatase (TRAP) staining in mandible section, the number of TRAP-positive odontoclasts and osteoclasts were histologically counted along the root and the alveolar bone surfaces of tooth, respectively. Odontoclasts increased until day 10 in mesial and furcation root surface, but did not increase in distal root surface. When compared odontoclast formation to osteoclast formation in mesial surface, osteoclasts peaked at day 3, and then decreased gradually, whereas odontoclasts were continuously increased until day 10. The number of odontoclasts was lower than that of osteoclasts before and after periodontitis induction. These indicate that periodontitis increased formation of odontoclasts as well as osteoclasts, but odontoclast formation occurs slower and weaker than that of osteoclasts.
참고문헌 (13)
- Harokopakis-Hajishengallis E, "Physiologic root resorption in primary teeth: molecular and histological events", J Oral Sci, 49, pp.1~12 (2007)
- Sahara N, "Cytodifferentiation of the odontoclast prior to the shedding of human deciduous teeth: an ultrastructural and cytochemical study", Anat Rec, 244, pp.33~49 (1996)
- Muto A, "Lineage-committed osteoclast precursors circulate in blood and settle down into bone", J Bone Miner Res, 26, pp.2978~2990 (2011)
- Yamaguchi M, "RANKL increase in compressed periodontal ligament cells from root resorption", J Dent Res, 85, pp.751~756 (2006)
- Tyrovola JB, "Root resorption and the OPG", RANKL, J Oral Sci, pp.2008, 50[DOI]
- Crespo Abelleira AC, "Morphological study of root surfaces in teeth with adult periodontitis", J Periodontol, 70, pp.1283~1291 (1999)
- Rodriguez-Pato RB, "Root resorption in chronic periodontitis:a morphometrical study", J Periodontol, 75, pp.1027~1032 (2004)
- Nakano Y, "Expressions of RANKL", RANK and M-CSF, Eur J Orthod, pp.2011, 33[DOI]
- Fukushima H, "Expression and role of RANKL in periodontal ligament cells during physiological root-resorption in human deciduous teeth", Eur J Oral Sci, 111, pp.346~352 (2003)
- Kim JH, "Receptor activator of nuclear factor-кB ligand and sclerostin expression in osteocytes of alveolar bone in rats with ligature-induced periodontitis", J Periodontol, 85, pp.e370~e378 (2014)
- Lee DE, "Periodontitis mainly increases osteoclast formation via enhancing the differentiation of quiescent osteoclast precursors into osteoclasts.", J Periodontal Res, 50, pp.256~264 (2015)
- Oka H, "F-spondin inhibits migration and differentiation of osteoclastic precursors.", J Periodontol, 82, pp.1776~1783 (2011)
- Duarte PM, "Microbial profile of ligature-induced periodontitis in rats", Arch Oral Biol, 55, pp.142~147 (2010)