Inhibition of Streptococcus mutans biofilm formation on composite resins containing ursolic acid
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Objectives: To evaluate the inhibitory effect of ursolic acid (UA)-containing composites on Streptococcus mutans (S. mutans) biofilm. Materials and Methods:Composite resins with five different concentrations (0.04, 0.1, 0.2, 0.5, and 1.0 wt%)of UA (U6753, Sigma Aldrich) were prepared, and their flexural strengths were measured according to ISO 4049. To evaluate the effect of carbohydrate source on biofilm formation, either glucose or sucrose was used as a nutrient source, and to investigate the effect of saliva treatment, the specimen were treated with either unstimulated whole saliva or phosphate-buffered saline (PBS). For biofilm assay, composite disks were transferred to S. mutans suspension and incubated for 24 hr. Afterwards, the specimens were rinsed with PBS and sonicated. The colony forming units (CFU) of the disrupted biofilm cultures were enumerated. For growth inhibition test, the composites were placed on a polystyrene well cluster, and S. mutans suspension was inoculated. The optical density at 600 nm (OD600) was recorded by Infinite F200pro apparatus (TECAN). One-way ANOVA and two-way ANOVA followed by Bonferroni correction were used for the data analyses. Results: The flexural strength values did not show significant difference at any concentration (p > 0.01). In biofilm assay, the CFU score decreased as the concentration of UA increased. The influence of saliva pretreatment was conflicting. The sucrose groups exhibited higher CFU score than glucose group (p < 0.05). In bacterial growth inhibition test, all experimental groups containing UA resulted in complete inhibition. Conclusions: Within the limitations of the experiments, UA included in the composite showed inhibitory effect on S. mutans biofilm formation and growth. (Restor Dent Endod 2013;38(2):65-72)
Abstract
Objectives: To evaluate the inhibitory effect of ursolic acid (UA)-containing composites on Streptococcus mutans (S. mutans) biofilm. Materials and Methods:Composite resins with five different concentrations (0.04, 0.1, 0.2, 0.5, and 1.0 wt%)of UA (U6753, Sigma Aldrich) were prepared, and their flexural strengths were measured according to ISO 4049. To evaluate the effect of carbohydrate source on biofilm formation, either glucose or sucrose was used as a nutrient source, and to investigate the effect of saliva treatment, the specimen were treated with either unstimulated whole saliva or phosphate-buffered saline (PBS). For biofilm assay, composite disks were transferred to S. mutans suspension and incubated for 24 hr. Afterwards, the specimens were rinsed with PBS and sonicated. The colony forming units (CFU) of the disrupted biofilm cultures were enumerated. For growth inhibition test, the composites were placed on a polystyrene well cluster, and S. mutans suspension was inoculated. The optical density at 600 nm (OD600) was recorded by Infinite F200pro apparatus (TECAN). One-way ANOVA and two-way ANOVA followed by Bonferroni correction were used for the data analyses. Results: The flexural strength values did not show significant difference at any concentration (p > 0.01). In biofilm assay, the CFU score decreased as the concentration of UA increased. The influence of saliva pretreatment was conflicting. The sucrose groups exhibited higher CFU score than glucose group (p < 0.05). In bacterial growth inhibition test, all experimental groups containing UA resulted in complete inhibition. Conclusions: Within the limitations of the experiments, UA included in the composite showed inhibitory effect on S. mutans biofilm formation and growth. (Restor Dent Endod 2013;38(2):65-72)
참고문헌 (26)
- Haj-Ali R, "Survey of general dentists regarding posterior restorations, selection criteria, and associated clinical problems", Gen Dent, 53, pp.369~375 (2005)
- Opdam NJ, "A retrospective clinical study on longevity of posterior composite and amalgam restorations", Dent Mater, 23, pp.2~8 (2007)
- Beyth N, "Antibacterial activity of dental composites containing quaternary ammonium polyethylenimine nanoparticles against Streptococcus mutans", Biomaterials, 27, pp.3995~4002 (2006)
- Leung D, "Chlorhexidine-releasing methacrylate dental composite materials", Biomaterials, 26, pp.7145~7153 (2005)
- Stobie N, "Prevention of Staphylococcus epidermidis biofilm formation using a low-temperature processed silver-doped phenyltriethoxysilane sol-gel coating", Biomaterials, 29, pp.963~969 (2008)
- Imazato S, "Incorporation of antibacterial monomer MDPB into dentin primer", J Dent Res, 76, pp.768~772 (1997)
- Fan C, "Development of an antimicrobial resin-a pilot study", Dent Mater, 27, pp.322~328 (2011)
- Nohr RS, "New biomaterials through surface segregation phenomenon: new quaternary ammonium compounds as antibacterial agents", J Biomater Sci Polym Ed, 5, pp.607~619 (1994)
- Liu J, "Oleanolic acid and ursolic acid: research perspectives", J Ethnopharmacol, 100, pp.92~94 (2005)
- Liu J, "Pharmacology of oleanolic acid and ursolic acid", J Ethnopharmacol, 49, pp.57~68 (1995)
- Fontanay S, "Ursolic, oleanolic and betulinic acids: antibacterial spectra and selectivity indexes", J Ethnopharmacol, 120, pp.272~276 (2008)
- Takahashi N, "The role of bacteria in the caries process: ecological perspectives", J Dent Res, 90, pp.294~303 (2011)
- Costerton JW, "Biofilms, the customized microniche", J Bacteriol, 176, pp.2137~2142 (1994)
- Shapiro S, "An in vitro oral biofilm model for comparing the efficacy of antimicrobial mouthrinses", Caries Res, 36, pp.93~100 (2002)
- Hardt M, "Assessing the effects of diurnal variation on the composition of human parotid saliva: quantitative analysis of native peptides using iTRAQ reagents", Anal Chem, 77, pp.4947~4954 (2005)
- Allaker RP, "Novel anti-microbial therapies for dental plaque-related diseases", Int J Antimicrob Agents, 33, pp.8~13 (2009)
- Jeon JG, "Natural products in caries research: current (limited) knowledge, challenges and future perspective", Caries Res, 45, pp.243~263 (2011)
- Min Jung Kim, "Antimicrobial Effects of Ursolic Acid against Mutans Streptococci Isolated from Koreans", International Journal of Oral Biology, 36(1), pp.7~11 (2011), 대한구강생물학회
- Cowan MM, "Plant products as antimicrobial agents", Clin Microbiol Rev, 12, pp.564~582 (1999)
- Kurek A, "Oleanolic acid and ursolic acid affect peptidoglycan metabolism in Listeria monocytogenes", Antonie Van Leeuwenhoek, 97, pp.61~68 (2010)
- Kohda H, "Prevention of dental caries by Oriental folk medicines-active principles of Zizyphi Fructus for inhibition of insoluble glucan formation by cariogenic bacterium Streptococcus mutans", Planta Med, 52, pp.119~120 (1986)
- Ooshima T, "Contributions of three glycosyltransferases to sucrose-dependent adherence of Streptococcus mutans", J Dent Res, 80, pp.1672~1677 (2001)
- Gibbons RJ, "Role of adhesion in microbial colonization of host tissues: a contribution of oral microbiology", J Dent Res, 75, pp.866~870 (1996)
- Cross SE, "Nanomechanical properties of glucans and associated cell-surface adhesion of Streptococcus mutans probed by atomic force microscopy under in situ conditions", Microbiology, 153, pp.3124~3132 (2007)
- Schilling KM, "Glucans synthesized in situ in experimental salivary pellicle function as specific binding sites for Streptococcus mutans", Infect Immun, 60, pp.284~295 (1992)
- Ren D, "Differential gene expression for investigation of Escherichia coli biofilm inhibition by plant extract ursolic acid", Appl Environ Microbiol, 71, pp.4022~4034 (2005)