성견의 3급 이개부 병변에서 성형성 합성 펩타이드 젤의 치주재생 능력에 관한 실험적 연구
Experimental study on the periodontal regenerative capacity of moldable synthetic peptide domain gel in degree Ⅲ furcation defect of beagles
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Purpose: Osteopontin is one of the major non-collagenous protein of hard tissue. Use of peptide domain of biologically active protein has some advantages. The objective of this experimental study is evaluation of periodontal regenerative potency of synthetic peptide gel which containing collagen binding domain of osteopontin in the degree III periodontal defect of beagle dogs. Material and Methods: Experimental degree III furcation defect was made in the mandibular third and fourth premolar of beagles. Regenerative material was applied during flap operation. 8 weeks after regenerative surgery, all animals were sacrificed and histomorphometric measurement was performed to calculate the linear percentage of the new cementum formation and the volume percentage of new bone formation. Result: The linear percent of new cementum formation was 41.6% at control group and 67.1% at test group and there was statistically significant difference. The volume percent of new bone formation was 52.1% at control group and 58.9% at test group. Conclusion: As the results of present experiment, synthetic peptide gel containing collagen binding domain of osteopontin significantly increase new bone and cementum formation in the degree III furcation defect of canine mandible. (J Korean Acad Periodontol 2008;38:621-628)
Abstract
Purpose: Osteopontin is one of the major non-collagenous protein of hard tissue. Use of peptide domain of biologically active protein has some advantages. The objective of this experimental study is evaluation of periodontal regenerative potency of synthetic peptide gel which containing collagen binding domain of osteopontin in the degree III periodontal defect of beagle dogs. Material and Methods: Experimental degree III furcation defect was made in the mandibular third and fourth premolar of beagles. Regenerative material was applied during flap operation. 8 weeks after regenerative surgery, all animals were sacrificed and histomorphometric measurement was performed to calculate the linear percentage of the new cementum formation and the volume percentage of new bone formation. Result: The linear percent of new cementum formation was 41.6% at control group and 67.1% at test group and there was statistically significant difference. The volume percent of new bone formation was 52.1% at control group and 58.9% at test group. Conclusion: As the results of present experiment, synthetic peptide gel containing collagen binding domain of osteopontin significantly increase new bone and cementum formation in the degree III furcation defect of canine mandible. (J Korean Acad Periodontol 2008;38:621-628)
참고문헌 (37)
- Page RC, "Pathogenesis of inflammatory periodontal disease. A summary of current work", Lab Invest, 33, pp.235~249 (1976)
- Flemmig TF, "Periodontitis", Ann Periodontol, 4, pp.32~37 (1999)
- Caton J, "Osseous repair of an infrabony pocket without new attachment of connective tissue", J Clin Periodontol, 3, pp.54~58 (1976)
- Garrett S, "Periodontal regeneration around natural teeth", Ann Periodontol, 1, pp.621~666 (1996)
- Reynolds MA, "The efficacy of bone replacement grafts in the treatment of periodontal osseous defects. A systematic review", Ann Periodontol, 8, pp.227~265 (2003)
- Melcher AH, "On the repair potential of periodontal tissues", J Periodontol, 47, pp.256~260 (1976)
- Gottlow J, "New attachment formation in the human periodontium by guided tissue regeneration. Case reports", J Clin Periodontol, 13, pp.604~616 (1986)
- Caffesse RG, "Class II furcations treated by guided tissue regeneration in humans: Case reports", J Periodontol, 61, pp.510~514 (1990)
- Cortellini P, "Guided tissue regeneration with different materials", Int J Periodontics Restorative Dent, 10, pp.137~151 (1990)
- Selvig KA, "Regenerative surgery of intrabony periodontal defects using ePTFE barrier membranes. Scanning electron microscopic evaluation of retrieved membranes versus clinical healing", J Periodontol, 63, pp.974~978 (1992)
- Murphy KG, "Guided tissue regeneration for the treatment of periodontal intrabony and furcation defects. A systematic review", Ann Periodontol, 8, pp.266~302 (2003)
- Hammarstrom L, "Origins of cementum", Oral Dis, 2, pp.63~69 (1996)
- Hammarstrom L, "Enamel matrix, cementum development and regeneration", J Clin Periodontol, 24, pp.658~668 (1997)
- Giannobile WV, "Growth and amelogenin- like factors in periodontal wound healing. A systematic review", Ann Periodontol, 8, pp.193~204 (2003)
- Esposito M, "Worthington HV. Enamel matrix derivative (Emdogainfor periodontal tissue regeneration in intrabony defects (Review)", Cochrane Database Syst Rev, 19 (2005)
- Butler WT, "The nature and functional significance of dentin extracellular matrix proteins", Int J Dev Biol, 39, pp.169~179 (1995)
- Gehron RP, "Structure and molecular regulation of bone matrix proteins", J Bone Miner Res, 8, pp.S483~S487 (1993)
- Butler WT, "Dentin matrix proteins and dentinogenesis", Connect Tissue Res, 33, pp.59~65 (1995)
- Veis A, "Mineral matrix interactions in bone and dentin", J Bone Miner Res, 8, pp.S493~S497 (1993)
- McKee MD, "Osteopontin at mineralized tissue interfaces in bone, teeth, and osseointegrated implants: Ultrastructural distribution and implications for mineralized tissue formation, turnover, and repair", Microsc Res Tech, 33, pp.141~164 (1996)
- Bosshardt DD, "Developmental appearance and distribution of bone sialoprotein and osteopontin in human and rat cementum", Anat Rec, 250, pp.13~33 (1998)
- Nanci A, "Content and distribution of noncollagenous matrix proteins in bone and cementum: relationship to speed of formation and collagen packing density", J Struct Biol, 126, pp.256~269 (1999)
- Kawaguchi H, "Immunodetection of noncollagenous matrix proteins during periodontal tissue regeneration", J Periodont Res, 36, pp.205~213 (2001)
- Lee JY, "Injectable gel with synthetic collagen binding peptide for enhanced osteogenesis in vitro and in vivo", Biochem Biophys Res Commun, 357, pp.68~74 (2007)
- Jun-Beom Park, "Osteopromotion with synthetic oligopeptidecoated bovine bone mineral in vivo", J Periodontol, 78, pp.157~163 (2007)
- Yang-Jo Seol, "Enhanced osteogenic promotion around dental implants with synthetic binding motif mimicking bone morphogenetic protein (BMP) - 2", J Biomed Mater Res, 77A, pp.599~607 (2006)
- Hersel U, "RGD modified polymers: biomaterials for stimulated cell adhesion and beyond", Biomaterials, 24, pp.4385~4415 (2003)
- Hamp SE, "Periodontal treatment of multirooted teeth. Results after 5 years", J Clin Periodontol, 2, pp.126~135 (1975)
- Lindhe J, "The effect of flap management and bioresorbable occlusive devices in GTR treatment of degree III furcation defects. An experimental study in dogs", J Clin Periodontol, 22, pp.276~283 (1995)
- Donath K, "A method for the study of undecalcified bones and teeth with attached soft tissues. The Sage-Schliff (sawing and grinding) technique", J Oral Pathol, 11, pp.318~326 (1982)
- Trombelli L, "Clinical outcomes with bioactive agents alone or in combination with grafting or guided tissue regeneration", J Clin Periodontol, 35(8), pp.117~135 (2008)
- Liaw L, "The adhesive and migratory effects of osteopontin are mediated via distinct cell surface integrins: role of αvβ3 in smooth muscle migration to osteopontin", J Clin Invest, 95, pp.713~724 (1995)
- Oldberg A, "Cloning and sequence analysis of rat bone sialoprotein (osteopontin) cDNA reveals an Arg-Gly-Asp cell binding sequence", Proc Natl Acad Sci USA, 83, pp.8819~8823 (1986)
- Tye CE, "Identification of the type I collagen-binding domain of bone sialoprotein and characterization of the mechanism of interaction", J Biol Chem, 280, pp.13487~13492 (2005)
- Ito S, "In vitro apatite induction by osteopontin: interfacial energy for hydroxyapatite nucleation on osteopontin", J Biomed Mater Res A, 69, pp.11~16 (2004)
- Araujo M, "The periodontal tissues in healed degree III furcation defects. An experimental study in dogs", J Clin Periodontol, 23, pp.532~541 (1996)
- Araujo MG, "GTR treatment of degree III furcation defects following application of enamel matrix proteins. An experimental study in dogs", J Clin Periodontol, 25, pp.524~530 (1998)
