Bone repair in defects filled with AH Plus sealer and different concentrations of MTA: a study in rat tibiae
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objectives: This study aimed to evaluate the effects on bone repair of different concentrations of mineral trioxide aggregate (MTA) added to AH Plus. Materials and Methods: Bone tissue reactions were evaluated in 30 rats (Rattus norvegicus) after 7 and 30 days. In the AH + MTA10, AH + MTA20, and AH + MTA30 groups, defects in the tibiae were filled with AH Plus with MTA in proportions of 10%, 20% and 30%, respectively; in the MTA-FILL group, MTA Fillapex was used; and in the control group, no sealer was used. The samples were histologically analyzed to assess bone union and maturation. The Kruskal-Wallis and Mann-Whitney tests were performed for multiple pairwise comparisons (p ≤ 0.05). Results: At the 7-day time point, AH + MTA10 was superior to MTA-FILL with respect to bone union, and AH + MTA20 was superior to MTA-FILL with respect to bone maturity (p < 0.05). At the 30-day time point, both the AH + MTA10 and AH + MTA20 experimental sealers were superior not only to MTA-FILL, but also to AH + MTA30 with respect to both parameters (p < 0.05). The results of the AH + MTA10 and AH + MTA20 groups were superior to those of the control group for both parameters and experimental time points (p < 0.05). Conclusions: The results suggest the potential benefit of using a combination of these materials in situations requiring bone repair.
Abstract
Objectives: This study aimed to evaluate the effects on bone repair of different concentrations of mineral trioxide aggregate (MTA) added to AH Plus. Materials and Methods: Bone tissue reactions were evaluated in 30 rats (Rattus norvegicus) after 7 and 30 days. In the AH + MTA10, AH + MTA20, and AH + MTA30 groups, defects in the tibiae were filled with AH Plus with MTA in proportions of 10%, 20% and 30%, respectively; in the MTA-FILL group, MTA Fillapex was used; and in the control group, no sealer was used. The samples were histologically analyzed to assess bone union and maturation. The Kruskal-Wallis and Mann-Whitney tests were performed for multiple pairwise comparisons (p ≤ 0.05). Results: At the 7-day time point, AH + MTA10 was superior to MTA-FILL with respect to bone union, and AH + MTA20 was superior to MTA-FILL with respect to bone maturity (p < 0.05). At the 30-day time point, both the AH + MTA10 and AH + MTA20 experimental sealers were superior not only to MTA-FILL, but also to AH + MTA30 with respect to both parameters (p < 0.05). The results of the AH + MTA10 and AH + MTA20 groups were superior to those of the control group for both parameters and experimental time points (p < 0.05). Conclusions: The results suggest the potential benefit of using a combination of these materials in situations requiring bone repair.
참고문헌 (25)
- Lim Miyoung, "Calcium silicate-based root canal sealers: a literature review", Restorative Dentistry and Endodontics, 45(3), pp.1~17 (2020), 대한치과보존학회[DOI]
- Fonseca DA, "Biocompatibility of root canal sealers: a systematic review of in vitro and in vivo studies", Materials (Basel), 12, pp.4113~4117 (2019)
- Al-Haddad A, "Bioceramic-based root canal sealers: a review", Int J Biomater, 2016, pp.9753210~ (2016)
- Kaur A, "Biotoxicity of commonly used root canal sealers: a meta-analysis", J Conserv Dent, 18, pp.83~88 (2015)
- Jafari F, "Composition and physicochemical properties of calcium silicate based sealers: a review article", J Clin Exp Dent, 9, pp.e1249~e1255 (2017)
- Torabinejad M, "Physical and chemical properties of a new root-end filling material", J Endod, 21, pp.349~353 (1995)
- Torabinejad M, "Mineral trioxide aggregate and other bioactive endodontic cements: an updated overview - part II: other clinical applications and complications", Int Endod J, 51, pp.284~317 (2018)
- Assmann E, "Evaluation of bone tissue response to a sealer containing mineral trioxide aggregate", J Endod, 41, pp.62~66 (2015)
- Jung S, "Cytotoxic effects of four different root canal sealers on human osteoblasts", PLoS One, 13, pp.e0194467~ (2018)
- Kebudi Benezra M, "Interfacial characteristics and cytocompatibility of hydraulic sealer cements", J Endod, 44, pp.1007~1017 (2018)
- Cintra LTA, "Evaluation of the cytotoxicity and biocompatibility of new resin epoxy-based endodontic sealer containing calcium hydroxide", J Endod, 43, pp.2088~2092 (2017)
- de Oliveira RL, "Influence of calcium hydroxide addition to AH Plus sealer on its biocompatibility", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 109, pp.e50~e54 (2010)
- Falcão LF, "Evaluation of the biocompatibility of AH Plus sealer associated to Portland cement or MTA. Subcutaneous study in rats", Rev Interd, 11, pp.1~11 (2018)
- Garcia LF, "Repair of bone defects filled with new calcium aluminate cement (EndoBinder)", J Endod, 41, pp.864~870 (2015)
- Falcão CA, "Plus adhesiveness assessment associated with mineral trioxide aggregate in different proportions (push-out test)", J Contemp Dent Pract, 19, pp.1444~1447 (2018)
- Hedner E, "Efficacy of bone morphogenetic protein (BMP) with osteopromotive membranes--an experimental study in rat mandibular defects", Eur J Oral Sci, 103, pp.236~241 (1995)
- Delfino MM, "Immunoinflammatory response and bioactive potential of GuttaFlow bioseal and MTA Fillapex in the rat subcutaneous tissue", Sci Rep, 10, pp.7173~ (2020)
- Sousa Filho JL, "Radiopacity of AH Plus endodontic sealer plus MTA and Portland cement", Dental Press Endod, 8, pp.18~21 (2018)
- Sarkar NK, "Physicochemical basis of the biologic properties of mineral trioxide aggregate", J Endod, 31, pp.97~100 (2005)
- Costa F, "Osteogenic and angiogenic response to calcium silicate–based endodontic sealers", J Endod, 42, pp.113~119 (2016)
- Letícia Boldrin Mestieri, "Cytocompatibility and cell proliferation evaluation of calcium phosphate-based root canal sealers", Restorative Dentistry and Endodontics, 45(1), pp.1~7 (2020), 대한치과보존학회[DOI]
- Salles LP, "Mineral trioxide aggregate-based endodontic sealer stimulates hydroxyapatite nucleation in human osteoblast-like cell culture", J Endod, 38, pp.971~976 (2012)
- Saraiva JA, "Reduced interleukin-6 immunoexpression and birefringent collagen formation indicate that MTA Plus and MTA Fillapex are biocompatible", Biomed Mater, 13, pp.035002~ (2018)
- Scelza MZ, "Evaluation of inflammatory response to endodontic sealers in a bone defect animal model", J Contemp Dent Pract, 17, pp.536~541 (2016)
- Almeida LH, "Bone tissue response to an MTA-based endodontic sealer, and the effect of the addition of calcium aluminate and silver particles", Int Endod J, 52, pp.1446~1456 (2019)