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Interleukin-8 and MCP(Monocyte Chemoattractant Protein)-1 expression by the Human Dental Pulps in cultures stimulated with Substance P

박상혁|최기운|신한주
대한치과보존학회 (Korean Academy Of Conservative Dentistry)|2005.05|Restorative Dentistry and Endodontics|제30권 제3호|pp. 193-203

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참고문헌

키워드

SP염증IL-8MCP-1치수 조직
주제분류치의학

초록

본 연구에서는 발거된 건전한 치아의 치수조직으로부터 배양된 치수조직을 SP 및TNF(tumor necrosis factor)-α, 그리고 Spantide로 15분간 배양 후 SP로 36시간 자극하여IL-8 및MCP-1의 분비량을 측정하였으며,면역염색으로 IL-8의 분비를 관찰하여 다음과 같은 결론을 얻었다. 1. 치수 조직을 SP(10-4M)로 36 시간 자극 시 모의 자극에 비해 IL-8이 현저히 증가하였으며 (p<0.05), 면역 염색 결과 모의 자극 시에는 치수조직의 변연부에만, SP(10-4M)로 36시간 자극시에는 fibroblast 주위로 IL-8의 발현이 관찰되었다. 2. TNF-α(40 ng/ml)로 치수조직을 36 시간 자극시 모의 자극에 비해 MCP-1이 증가하였으며, SP에서는 증가를 보이지 않았으며(p>0.05), 면역 염색 결과 IL-8의 발현이 관찰되지 않았으며, 치수 조직의 변연부를 따라서 약한 IL-8의 발현이 관찰되었다. 3. Spantide(10-5 M)는 SP(10-4 M)로 치수 조직을 36 시간 자극 시 IL-8의 분비를 억제하였다.

Abstract

The induction of the IL-8 and MCP-1 by the stimulation of Substance P and TNF-α(IL-8 agonist) and the specificity for SP using Spantide(SP antagonist) in the dental pulp tissues was measured quantitatively. In addition, the secretion of the IL-8 in the human dental pulp tissue 36 hrs after the stimulation of SP was observed after the stimulation of SP qualitatively. According to this study, the results were as follows: 1. There was the significant IL-8 induction at 36 h after SP(10-4M) stimulation of the pulp tissue comparing with the unstimulated dental pulp tissues.(p<0.05) IL-8 immunostaining was weakly detected along the periphery of the pulp tissue after Mock stimulation and IL-8 immunostaining was detected around the fibroblast in the pulp tissue 36h After SP(10-4M) stimulation, 2. The secretion of MCP-1 from the dental pulp tissues comparing with Mock stimulation was induced at 36 hrs after TNF-α(40ng/ml) stimulation, but no induction with SP(10-4M). TNF-α(40ng/ml) did not induce the IL-8 secretion from the pulp tissue, weak IL-8 immunostaining was detected along the periphery of the pulp tissue. 3. Spantide(10-5M) inhibited IL-8 induction from the pulp tissues 36 h after SP(10-4M) stimulation. These results suggest that SP significantly induces IL-8 recruiting neutrophils in localized human dental pulp tissue. MCP-1 appears to be less involved in the early establishment of pulpal inflammation in response to irritation such as mechanical insult of dentin. SP may have positive relation with the inflammation of the human dental pulp tissues.

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관련 논문AI추천

치수세포의 Substance P 자극 시 Interleukin-8과 Monocyte chemattractant protein-1의 분비에 관한 연구박상혁대한치과보존학회 · Restorative Dentistry and Endodontics2003 · 제28권 제5호Expression of Interleukin-8 and monocyte chemoattractant protein-1 in the Human Dental Pulps after stimulation with Substance P이희수대한구강해부학회 · 대한구강해부학회지2010 · 제32권 제1호사람치수조직에서 Substance P 에 의한Interleukin-8의 발현에 대한 면역조직화학적인 연구H. S. Lee, S. H. Park대한구강해부학회 · 대한구강해부학회지2005 · 제29권 제2호사람의 치수, 치은, 치주인대 세포에 tumor necrosis factor (TNF)-α로 자극 시 matrix metalloproteinase (MMPs)의 분비에 관한 연구김덕수, 김선영, 박상혁, 임은미, 최경규, 최기운대한치과보존학회 · Restorative Dentistry and Endodontics2011 · 제36권 제1호근관 삼출액에서의 기질금속단백분해효소-8과 substance P의 수준에 관한 연구금기연, 배광식, 백승호, 손원준, 신수정, 이우철, 이재일대한치과보존학회 · Restorative Dentistry and Endodontics2011 · 제36권 제3호성인 치수염에서의 INTERLEUKIN-6와 INTERLEUKIN-10의 작용에 관한 연구박한수대한치과보존학회 · Restorative Dentistry and Endodontics2002 · 제27권 제5호

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