Expression of Interleukin-8 and monocyte chemoattractant protein-1 in the Human Dental Pulps after stimulation with Substance P
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Aim : To investigate whether leukocyte infiltration during neurogenic inflammation in the pulp is regulated by neuropeptides via inducing the release of proinflammatory chemokines interleukin-8 (IL-8) from human dental pulp , Methods : Primary human dental pulp tissue explants were stimulated with substance P (SP) , IL-8 produced in pulp explants was examined by immuno- histochemistry. Results : After the pulp tissues explants were cultured for 12 h and 24 h in the medium without FBS, IL-8 were expressed only around the cell rich zone and blood vessels in the pulp tissues , SP(10-4 M) induced lL-8 expression in the pulp tissue explants after 24 h of SP stimulation, while TNF-a(20ng/ml) did not induce the IL-8 in the pulp tissue. The stronger lL-8 staining was detected in the pulp tissue explants after 6 and 12 h of TNF-a(20ng/mI) stimulation, while only dead cells were revealed in the pulp tissue after 24 h of TNF-a(20ng/mI) stimulation. Conc1usions : SP significantly induces IL-8 expression in human dental pulp suggesting an important role of SP in recruiting neutrophils in localized pulp tissue , SP may have positive relation with the inflammation of the human dental pulp tissues.
Abstract
Aim : To investigate whether leukocyte infiltration during neurogenic inflammation in the pulp is regulated by neuropeptides via inducing the release of proinflammatory chemokines interleukin-8 (IL-8) from human dental pulp , Methods : Primary human dental pulp tissue explants were stimulated with substance P (SP) , IL-8 produced in pulp explants was examined by immuno- histochemistry. Results : After the pulp tissues explants were cultured for 12 h and 24 h in the medium without FBS, IL-8 were expressed only around the cell rich zone and blood vessels in the pulp tissues , SP(10-4 M) induced lL-8 expression in the pulp tissue explants after 24 h of SP stimulation, while TNF-a(20ng/ml) did not induce the IL-8 in the pulp tissue. The stronger lL-8 staining was detected in the pulp tissue explants after 6 and 12 h of TNF-a(20ng/mI) stimulation, while only dead cells were revealed in the pulp tissue after 24 h of TNF-a(20ng/mI) stimulation. Conc1usions : SP significantly induces IL-8 expression in human dental pulp suggesting an important role of SP in recruiting neutrophils in localized pulp tissue , SP may have positive relation with the inflammation of the human dental pulp tissues.
참고문헌 (9)
- Bergenholtz G, "Effect of soluble plaque factors on inflammatory reactions in the dental pulp", Scandinavian Journal of Dental Research, 83, pp.153~158 (1975)
- Stashenko P, "Periapical inflammatory responses and their modulation", Critical Reviews in Oral Biology and Medicine, 9, pp.498~521 (1998)
- Muller WA, "Migration of leukocytes across endothelium and beyond: molecules involved in the transrnigration and fate of monocytes", Journal of Leukocyte Biology, 66, pp.698~704 (1999)
- Wakisaka S, "Neuropeptides in the dental pulp: distribution, origins, and correlation", JoumaJ of Endodontics, 16, pp.67~69 (1990)
- Heyeraas KJ, "Effect of electrical tooth stimulation on blood flow, interstitial fluid pressure and substance P and CGRP-immunoreactive nerve fibers in the low compliant cat dental pulp", Microvascular Research, 47, pp.329~343 (1994)
- Fristad I, "Effect of intermittent long-lasting electrical tooth stimulation on pulpal blood flow and immunocompetent cells: a hemodynarnic and immunohistochernical study in young rat molars", Experimental Neurology, 146, pp.230~239 (1997)
- Huang GTJ, "Increased interleukin-8 expression in inflamed human dental pulps", Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, 88, pp.214~220 (1999)
- Awawdeh L, "Quantitative analysis of substance P, neurokinin A and calcitonin gene-related peptide in pulp tissue from painful and healthy human teeth", International Endodontic Journal, 35, pp.30~36 (2002)
- Patel T, "Substance P induces interleukin-8 production from human dental pulp cells", Oral Surgery, Oral Medícine, Oral Pathology, Oral Radiology, And Endodontics, 96, pp.478~485 (2003)