사람치수조직에서 Substance P 에 의한Interleukin-8의 발현에 대한 면역조직화학적인 연구

Abstract

Aim To investigate whether leukocyte infiltration during neurogenic inf1ammation in the pulp is regulated by neuropeptides via inducing the release of proinflammatory chemokines interleukin-8 (江- 8) from human dental pulp. we employed an ex vivo pulp explant system and immunohistochemical methods to determine whether induct퍼 IL -8 protein expression can be detected and located in situ in the SP-stimulated pulp tissue. Methodology Primary human dental pulp tissue explants were stimulated with subst:ance P (SP). IL-8 produced in pulp explants was exarnined by immunohistochernistry. Resu1ts After the pulp tissues explants were cultured for 12 h and 24 h in the medium without FBS, IL-8 were expressed only around the cell rich zone and blood vessels in the pulp tissues. SP(10-4 M) induct최 IL-8 expression in the pulp tissue explants after 24 h of SP stimulation. ι-8 immunohistochemica1 analysis of frozen p비p explant 12 h after SP (10-4 M) stimulation. SP(10-4 M) stimulated pulp explant tissue exhibits 1L-8 immunoreactivity only a10ng the border of the tissue. The center of SP (10-' M) stimulated pulp explant exhibits weak immunoreactivity. Mo다( stimulated pulp explant tissue e앙디 bits IL-8 immunoreactivity only a10ng the border of the tissue 江8 immunohistochemica1 analysis of frozen pulp explant 24 h after SP 00-4 M) stimulation. SP (10-4 M) stimulated pulp explant tissue exhibits strong IL -8 immunoreactivity around the fibroblasts of the tissue. The center of SP (10-4 M) stimulated pulp explant exhibits strong immunoreactivity. Higher-power view of explant pulp tissue from same sample shows strong IL-8 stai띠 ng in pulp cells and extracellular matrix of the tissue. Conc1usions SP significantly induces IL-8 expression in human dental pulp suggesting an important role of SP in recruiting neutrophils in loca1ized pulp tissue. SP may have Püsitive relation with the inflammation of the human dental pulp tissues