괴화 추출물 루틴의 소염작용: 쥐의 복강대식세포로부터 NO와 TNF-alpha 생산에 있어서 괴화 루틴에 의한 억제효과
Anti-inflammatory Function of the Sophora japonica Extract Rutin: The Inhibitory Effect of Rutin of Korean Sophora japonica on the Productions of NO and TNF-alpha from Mouse Peritoneal Macrophages
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초록
Korean Sophora japonica has been found to posses an anti-inflammatory activity. In this study, Korean Sophora japonica extract, rutin, was used to know whether rutin inhibits to produce inflammatory mediators NO and TNF-α from the mouse peritoneal macrophages that were treated an inflammatory agent LPS. The rutin-1 hr pretreated macrophages were incubated with LPS for 0.5~5 hrs, and then collected the supernatant and the cell lysate for measurements of the level of iNOS, NO, TNF-α mRNA, TNF-α, and p-NF-κB. Minimal and maximal effective doses of the rutin on them were 1 and 100㎍/㎖, respectively. The maximal effective dose of rutin certainly inhibted the productions of iNOS, NO, TNF-αmRNA, TNF-α, and p-NF-κB from the LPS-treated macrophages (p < 0.0001). Its ED50 for inhibition of TNF-α mRNA and p-NF-κB was 5㎍/㎖, and for iNOS, NO, and TNF-α was 10㎍/㎖. The rutin did not have any cytotoxic effect. As the results, the Sophora japonica rutin could be a good candidate for an anti-inflammatory action.
Abstract
Korean Sophora japonica has been found to posses an anti-inflammatory activity. In this study, Korean Sophora japonica extract, rutin, was used to know whether rutin inhibits to produce inflammatory mediators NO and TNF-α from the mouse peritoneal macrophages that were treated an inflammatory agent LPS. The rutin-1 hr pretreated macrophages were incubated with LPS for 0.5~5 hrs, and then collected the supernatant and the cell lysate for measurements of the level of iNOS, NO, TNF-α mRNA, TNF-α, and p-NF-κB. Minimal and maximal effective doses of the rutin on them were 1 and 100㎍/㎖, respectively. The maximal effective dose of rutin certainly inhibted the productions of iNOS, NO, TNF-αmRNA, TNF-α, and p-NF-κB from the LPS-treated macrophages (p < 0.0001). Its ED50 for inhibition of TNF-α mRNA and p-NF-κB was 5㎍/㎖, and for iNOS, NO, and TNF-α was 10㎍/㎖. The rutin did not have any cytotoxic effect. As the results, the Sophora japonica rutin could be a good candidate for an anti-inflammatory action.
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